[**Virtual University Of Pakistan**Student Cafe] .::VULMSIT::.eNoxel Good Bye MSCHOTAMASTER

Aslam u Alakum!
 
Friends Finally I have completed my degree and got job in lahore. So Now it is time to say Good Bye. AnyOne who got disterbance etc through my behavior or mistakes Forgive me I have forgived all.Anyone want any help in .net envoirment frequently call me on 03456550981 or send email on mschotamaster@gmail.com
Remember me in ur prayers
 
Allah Hafiz
Regards
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[DIYbio] Re: [diybio-boston] diybio open hardware community?

There's now DIY hardware communities which promote everything as open (http://sharism.org/en/presents) ... they call it "gifts of knowledge". These operate a copyleft hardware design shop (http://en.qi-hardware.com/wiki/Ben_NanoNote) which document everything from board schematics, build process to Bill of Materials. Microscopy with complex lens arrangements might be a little tricky for them unless you want lens on a fibre. Costs are reduced through a book build commit to minimum size orders before ordering components and production.

Lawrence
http://www.linkedin.com/in/drllau

On Sunday, 1 April 2012 06:24:55 UTC+13, Bryan Bishop wrote

Date: Sat, Mar 31, 2012 at 11:50 AM
Subject: Re: [diybio-boston] diybio open hardware community?
To: diybio-boston@googlegroups.com


I'm actually looking at building my own modular microscopy system from scratch.  Modifying premade microscopes is definitely cheaper, but much less flexible if you want to add filters/optics/etc. for fancy imaging techniques.  I'm starting my design by building a research-quality scope first and then figuring out how to minimize the costs to make it more affordable.

My DIY fluorescence microscope probably runs around $2k is my guess -- about an order of magnitude cheaper than competing microscopes by Olympus/Nikon/etc., but still too expensive for individuals.
http://asymptoticdesign.wordpress.com/2012/03/10/diy-scope/

I'm also workign on software to go with it:
http://asymptoticdesign.wordpress.com/2012/03/29/openscope-software-v1-0/

I'm currently working on documenting the fluorescence imaging and adding a confocal microscopy extension to it, and then it's going to be all about how to minimize costs.

nathan


On Sat, 31 Mar 2012, Christopher Saenz wrote:

No but let's get on it. I am relatively new to stuff at sprout. I went a couple of times but then got busy  finishing my degree. I just graduated and now can dedicate some time to this stuff. I polished up the epi the last time i was there. what were you looking at doing with microscopy?

chris


On Mar 31, 2012, at 12:05 PM, nathan lachenmyer wrote:

Does anyone know of any open hardware communities; especially ones that are diybio oriented?  I'd like to find a community to both share and collaborate on open hardware tools for biology, but most of the openhardware communities I can find aren't really science oriented.

I've been working on a few projects (in particular, open source microscopy) and would be interested to find other people to check out/test/etc. my projects with.

Thanks,

Nathan

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On Sunday, 1 April 2012 06:24:55 UTC+13, Bryan Bishop wrote:
 
Date: Sat, Mar 31, 2012 at 11:50 AM
Subject: Re: [diybio-boston] diybio open hardware community?
To: diybio-boston@googlegroups.com


I'm actually looking at building my own modular microscopy system from scratch.  Modifying premade microscopes is definitely cheaper, but much less flexible if you want to add filters/optics/etc. for fancy imaging techniques.  I'm starting my design by building a research-quality scope first and then figuring out how to minimize the costs to make it more affordable.

My DIY fluorescence microscope probably runs around $2k is my guess -- about an order of magnitude cheaper than competing microscopes by Olympus/Nikon/etc., but still too expensive for individuals.
http://asymptoticdesign.wordpress.com/2012/03/10/diy-scope/

I'm also workign on software to go with it:
http://asymptoticdesign.wordpress.com/2012/03/29/openscope-software-v1-0/

I'm currently working on documenting the fluorescence imaging and adding a confocal microscopy extension to it, and then it's going to be all about how to minimize costs.

nathan


On Sat, 31 Mar 2012, Christopher Saenz wrote:

No but let's get on it. I am relatively new to stuff at sprout. I went a couple of times but then got busy  finishing my degree. I just graduated and now can dedicate some time to this stuff. I polished up the epi the last time i was there. what were you looking at doing with microscopy?

chris


On Mar 31, 2012, at 12:05 PM, nathan lachenmyer wrote:

Does anyone know of any open hardware communities; especially ones that are diybio oriented?  I'd like to find a community to both share and collaborate on open hardware tools for biology, but most of the openhardware communities I can find aren't really science oriented.

I've been working on a few projects (in particular, open source microscopy) and would be interested to find other people to check out/test/etc. my projects with.

Thanks,

Nathan

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Re: [DIYbio] Bioluminescent yogurt (Again!)

googling "electroporation acidophilus" gives lots of results, the first says electroporation yieled "7.4 × 106 transformants per μg of plasmid DNA under optimized conditions"... so basically you should have no problems transforming small circular or linear DNA.


I'm also on the genomic integration is better" boat. Once its in there, as long as the genes are toxic or draining on the cell. constitutive promoters could 'drain'/strain the cell because of a constant amino acid demand... so maybe you want a low expression constitutive, or something thats only induced. Maybe lac promoter could work, so induction at least tapers off as incubation time increases.

On Sat, Mar 31, 2012 at 10:38 AM, medminus9 <harshsethia1989@gmail.com> wrote:
Hello all!

I am working as an instructor to a HS iGEM team and as a part of their project I am helping them design bioluminescent yogurt. I have been through the previous thread and all the blog posts attached to the thread, but found that most of the ideas were lacking references and supportive literature. So, this is what i have proposed after going through the literature, your inputs and help will be much appreciated! :))
  • Acetaldehyde: Acetaldehyde can react with luciferase complex to produce bioluminescence. Since acetaldehyde is produced by lactobacillus and streptococcus thermophilus in abundance, it will be an ideal substrate for the luciferase to chew-on. It is also among the most abundant chemicals on Earth and will help reducing the size of our construct from luxCDABEG to luxABG.
http://www.jbmb.or.kr/jbmb/jbmb_files/[20-1]0204292121_02600541.pdf 
  • Acidophilus: I am planning to work with acidophilus for the following reasons:
  1. Peroxide tolerance: Is more tolerant to H202 in compare to bulgaricus and several other species. Since bioluminescence will require aerobic conditions, the yogurt bacteria's will produce plenty of peroxide.
  2. Research: The bacteria has been thoroughly studied.
  3. Cost: My friend is making it available to me for free. He has been doing some research before.
  4. H202: Since acidophilus is H202 producing, the H202 can serve as an oxidizing agent required to form the flavin-peroxy intermediate. (Not sure!)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC84537/?tool=pmcentrez 
  • NAD(P)H2.FMN oxidoreductase: It is found in some species of acidophilus. I can't find the literature for this, but I do happen to have read it somewhere. Also most of the L.casei species do have this enzyme so I believe we if required we could even add the particular enzyme to the construct. 
Problems:
  1. Cathal in his blog post is favoring a linear transfer, but most of the literature is strongly against transformation with linear DNA. So, now I am confused in choosing between a plasmid transfer with chemical/ natural competence or a linear transfer?
  2. I am searching for a constitutive promoter for the purpose and would really appreciate any help with this.
  3. Is there anything which I am missing or might have not looked over. Your suggestions please! :)

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Re: [DIYbio] Fwd: [diybio-boston] diybio open hardware community?

On Sat, Mar 31, 2012 at 12:32 PM, Simon Quellen Field <sfield@scitoys.com> wrote:

I am also working on inexpensive home-brew microscopy.

Well? Let's see it? By see I mean plans, but pics are cool too...

- Bryan
http://heybryan.org/
1 512 203 0507

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Re: [DIYbio] Fwd: [diybio-boston] diybio open hardware community?

I am also working on inexpensive home-brew microscopy.


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Get a free science project every week! "http://scitoys.com/newsletter.html"




On Sat, Mar 31, 2012 at 10:24 AM, Bryan Bishop <kanzure@gmail.com> wrote:

From: nathan lachenmyer <scottnla@mit.edu>
Date: Sat, Mar 31, 2012 at 11:50 AM
Subject: Re: [diybio-boston] diybio open hardware community?
To: diybio-boston@googlegroups.com


I'm actually looking at building my own modular microscopy system from scratch.  Modifying premade microscopes is definitely cheaper, but much less flexible if you want to add filters/optics/etc. for fancy imaging techniques.  I'm starting my design by building a research-quality scope first and then figuring out how to minimize the costs to make it more affordable.

My DIY fluorescence microscope probably runs around $2k is my guess -- about an order of magnitude cheaper than competing microscopes by Olympus/Nikon/etc., but still too expensive for individuals.
http://asymptoticdesign.wordpress.com/2012/03/10/diy-scope/

I'm also workign on software to go with it:
http://asymptoticdesign.wordpress.com/2012/03/29/openscope-software-v1-0/

I'm currently working on documenting the fluorescence imaging and adding a confocal microscopy extension to it, and then it's going to be all about how to minimize costs.

nathan


On Sat, 31 Mar 2012, Christopher Saenz wrote:

No but let's get on it. I am relatively new to stuff at sprout. I went a couple of times but then got busy  finishing my degree. I just graduated and now can dedicate some time to this stuff. I polished up the epi the last time i was there. what were you looking at doing with microscopy?

chris


On Mar 31, 2012, at 12:05 PM, nathan lachenmyer wrote:

Does anyone know of any open hardware communities; especially ones that are diybio oriented?  I'd like to find a community to both share and collaborate on open hardware tools for biology, but most of the openhardware communities I can find aren't really science oriented.

I've been working on a few projects (in particular, open source microscopy) and would be interested to find other people to check out/test/etc. my projects with.

Thanks,

Nathan

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[DIYbio] Fwd: [diybio-boston] diybio open hardware community?


From: nathan lachenmyer <scottnla@mit.edu>

Date: Sat, Mar 31, 2012 at 11:50 AM
Subject: Re: [diybio-boston] diybio open hardware community?
To: diybio-boston@googlegroups.com


I'm actually looking at building my own modular microscopy system from scratch.  Modifying premade microscopes is definitely cheaper, but much less flexible if you want to add filters/optics/etc. for fancy imaging techniques.  I'm starting my design by building a research-quality scope first and then figuring out how to minimize the costs to make it more affordable.

My DIY fluorescence microscope probably runs around $2k is my guess -- about an order of magnitude cheaper than competing microscopes by Olympus/Nikon/etc., but still too expensive for individuals.
http://asymptoticdesign.wordpress.com/2012/03/10/diy-scope/

I'm also workign on software to go with it:
http://asymptoticdesign.wordpress.com/2012/03/29/openscope-software-v1-0/

I'm currently working on documenting the fluorescence imaging and adding a confocal microscopy extension to it, and then it's going to be all about how to minimize costs.

nathan


On Sat, 31 Mar 2012, Christopher Saenz wrote:

No but let's get on it. I am relatively new to stuff at sprout. I went a couple of times but then got busy  finishing my degree. I just graduated and now can dedicate some time to this stuff. I polished up the epi the last time i was there. what were you looking at doing with microscopy?

chris


On Mar 31, 2012, at 12:05 PM, nathan lachenmyer wrote:

Does anyone know of any open hardware communities; especially ones that are diybio oriented?  I'd like to find a community to both share and collaborate on open hardware tools for biology, but most of the openhardware communities I can find aren't really science oriented.

I've been working on a few projects (in particular, open source microscopy) and would be interested to find other people to check out/test/etc. my projects with.

Thanks,

Nathan

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[**Virtual University Of Pakistan**Student Cafe] .::VULMSIT::.eNoxel Girlfriend and Friend ........... hahahahahhahahaha



G/Friend: Plz Don't Drink & Drive, Accident Bohot
Ho Rahe Hain Aajkal.. ..
 
 
 
 
 
 
 
 
 
 
 
 
Boy: Thanks Baby, You're So Caring...... 
 
 
 
 
 
 
 
 
 
 
 
 
Friend: Plz Don't Drink & Drive, Accident Bohot
Ho Rahe Hain Aajkal....

 
 
 
 
 
 
 
 
 
 
 
 
 
Boy: Pata Hai Beta, Apne Baap Ko Mat Sikha..!!!  
 

 
 
 
The One & Only .......... IRFAN.
Italy.
 

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Re: [DIYbio] Re: query from PBS NewsHour


On Mar 31, 2012 5:01 AM, "Nathan McCorkle" <nmz787@gmail.com> wrote:
>
> On Sat, Mar 31, 2012 at 3:11 AM, Joseph Jackson <joseph.jackson@gmail.com> wrote:
> > We will eventually get to the promised land of synthetic biology, nanotech,
> > etc etc but in the mean time, a lot of people need to take a damn chill
> > pill.  
>
> Thumbs Up to that.

yes, well said.

Jason


>
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> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics
>
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[DIYbio] Bioluminescent yogurt (Again!)

Hello all!


I am working as an instructor to a HS iGEM team and as a part of their project I am helping them design bioluminescent yogurt. I have been through the previous thread and all the blog posts attached to the thread, but found that most of the ideas were lacking references and supportive literature. So, this is what i have proposed after going through the literature, your inputs and help will be much appreciated! :))
  • Acetaldehyde: Acetaldehyde can react with luciferase complex to produce bioluminescence. Since acetaldehyde is produced by lactobacillus and streptococcus thermophilus in abundance, it will be an ideal substrate for the luciferase to chew-on. It is also among the most abundant chemicals on Earth and will help reducing the size of our construct from luxCDABEG to luxABG.
http://www.jbmb.or.kr/jbmb/jbmb_files/[20-1]0204292121_02600541.pdf 
  • Acidophilus: I am planning to work with acidophilus for the following reasons:
  1. Peroxide tolerance: Is more tolerant to H202 in compare to bulgaricus and several other species. Since bioluminescence will require aerobic conditions, the yogurt bacteria's will produce plenty of peroxide.
  2. Research: The bacteria has been thoroughly studied.
  3. Cost: My friend is making it available to me for free. He has been doing some research before.
  4. H202: Since acidophilus is H202 producing, the H202 can serve as an oxidizing agent required to form the flavin-peroxy intermediate. (Not sure!)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC84537/?tool=pmcentrez 
  • NAD(P)H2.FMN oxidoreductase: It is found in some species of acidophilus. I can't find the literature for this, but I do happen to have read it somewhere. Also most of the L.casei species do have this enzyme so I believe we if required we could even add the particular enzyme to the construct. 
Problems:
  1. Cathal in his blog post is favoring a linear transfer, but most of the literature is strongly against transformation with linear DNA. So, now I am confused in choosing between a plasmid transfer with chemical/ natural competence or a linear transfer?
  2. I am searching for a constitutive promoter for the purpose and would really appreciate any help with this.
  3. Is there anything which I am missing or might have not looked over. Your suggestions please! :)

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[DIYbio] DIYbio and Bioethics {entering soft-science}

I was wondering if anyone is a hobbyist philosopher(or related, not
sure the name) who is studying Bioethics (the whole field rather than
DIYbio only). Why should DIYbio be confined to "hard" science like
labwork or in-silico? Is anyone, as a hobby, thinking and writing on
morals and ethics in biology and other science fields?

***Sorry if my words are not well-chosen, e.g. "soft-science",
"confined", etc. I hope my point is understandable.

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[**Virtual University Of Pakistan**Student Cafe] ..::VU-Pink::.. ``**``Yesterday Daily Quran and Hadith 30 March 2012...``**``


IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

 

 



 




 

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"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""
 
 
*~*$H!N!NG $T@R*~*
SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

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[**Virtual University Of Pakistan**Student Cafe] [Pak Youth] ``**``Yesterday Daily Quran and Hadith 30 March 2012...``**``


IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

 

 



 




 

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"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""
 
 
*~*$H!N!NG $T@R*~*
SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

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[**Virtual University Of Pakistan**Student Cafe] ~*~νυ ѕтυ∂у мαηια~*~ ``**``Daily Quran and Hadith 31 March 2012...``**``


IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

 

 



 




 

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"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""
 
 
*~*$H!N!NG $T@R*~*
SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

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[**Virtual University Of Pakistan**Student Cafe] ..::VU-Pink::.. ``**``Daily Quran and Hadith 31 March 2012...``**``


IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

 

 



 




 

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"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""
 
 
*~*$H!N!NG $T@R*~*
SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

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[**Virtual University Of Pakistan**Student Cafe] [Pak Youth] ``**``Daily Quran and Hadith 31 March 2012...``**``


IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

 

 



 




 

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"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""
 
 
*~*$H!N!NG $T@R*~*
SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

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[**Virtual University Of Pakistan**Student Cafe] Re: .::VULMSIT::.eNoxel صومالیہ میں نائٹ کلب کے مالک عبد اللہ کی خانہ کعبہ کا موذن بننے کی کہان

JAZAAK-ALLAH
 
Dear Atif sb ALLAH ap ko jaza-e-khair day. Ap k Article read karny k kabil hoty hain.
 
 
Regards
 
Imran Sheikh
MIT-MCS (VU)
 
Sr. Programmer
TMC-GTM-FSD

2012/3/31 Atif Aleem <atifalm@gmail.com>




صومالیہ میں نائٹ کلب کے مالک عبد اللہ کی خانہ کعبہ کا موذن بننے کی کہانی



میں ایک مشہور گلوکار تها لیکن پهر جب میں جب نائٹ کلب کا مالک بن گیا تو پھر وہاں گانے گاتا مقدیشو کے ہوٹل اور نائٹ کلب میری بکنگ کے لیے زیادہ سے زیادہ رقومات پیش کرتے۔ 

راتوں کو زندہ کرنے کیلئے، لوگوں کو خوش کرنے کیلئے اور اپنے آپ کو مزید پاپولر بنانے کیلئے میں نت نئے ناٹک رچاتا۔ عریاں ڈانس، فحش مکالمات اور عشقیہ گیتوں کے ذریعے پیسہ کمانا 

ہمارا مقصد حیات بن چکا تھا۔ جب یہ چیزیں میسر ہوں تو پھر شیطان خوب خوش ہوتا ہے۔ بگڑے ہوئے گھرانے، ان کی امیر لڑکیاں، اور لڑکے، شراب، نشہ، ہیروئن سب کچھ میسر تھا۔ رقص 

گاہیں ہماری وجہ سے آباد تھیں۔ شیطان کے اہداف حاصل کرنے کیلئے ہمارے ارد گرد بدکار لوگوں کا ایک بڑا گروہ تھا

ہم نے بھی اسلامی اقدار کو ختم کرنے اور شیطانی مجالس کو فروغ دینے میں ساری قوتیں صرف کردیں۔ ہم صرف نام کے مسلمان تھے۔ اسلامی روح کے بغیر ظاہری حد تک میں نے کتنے ہی یورپی ممالک کا سفر کیا ۔ وہاں نائٹ کلبوں میں گاتا رہا، صومال کے آرٹ کو اجاگر کرتا رہا، مغرب کو خوش کرنے کیلئے کہ ہم ترقی پسند قوم ہیں میرے ایمان کا، اسلام کا اور اخلاق کا جنازہ

 نکلتا گیا…..مگر میری جیب بھرتی گئی۔



"1983 ء میں میرے والد نے اپنے ہی خاندان میں سے ایک لڑکی کا انتخاب کیا۔ خوب ہلاگلا ہوا۔ ٹیلی ویژن، اخبارات، ذرائع ابلاغ کے نمائندے جمع ہوئے۔ یقینا یہ ایک یادگار شادی تھی۔

"شادی کے دوران میں، میں نے یہ محسوس کیا کہ میری بیوی اتنی زیادہ خوش و خرم نہیں ہے جتنا کہ میرے جیسے معروف آدمی سے شادی کے بعد کسی لڑکی کو خوش اور فخرہونا چاہیے۔ 

میں نے اس کو اس کی فطری حیا پر محمول کیا میں نے کئی مرتبہ دیکھا کہ جب صبح گھر آتا ہوں تو میری بیوی جاگ رہی ہوتی ہے اور عموماً اس کے ہاتھ میں قرآن پاک ہوتا ہے جسے
 وہ 
پڑھ رہی ہوتی ہے۔ میں آکر اسے بڑے جوشیلے انداز میں اس رات کی کارکردگی سناتا۔ اپنے پرستاروں کی چاہت سے آگاہ کرتا۔ آج کتنی لڑکیوں اور لڑکیوں کے فون آئے جو میرے فن کے 

شیدائی ہیں۔ میری بیوی ان باتوں کو ناگواری سے سنتی اور میرے لیے ہدایت کی دعا کرتی۔ اس دوران میں فجر کی اذان ہوجاتی اور وہ مصلے کی طرف بڑھ جاتی، جب کہ میں نماز پڑھے بغیر 

ہی سوجاتا۔ میں جب بھی اس سے نائٹ کلب کا ذکر کرتا، وہاں کی باتیں سنانا، اپنی کمائی کا ذکر کرتا، بینک بیلنس کا رعب جماتا تو وہ جواباً کہتی: "رازق تو صرف اللہ کی ذات ہے"

"ہماری شادی کو پانچ سال گزر چکے تھے۔ میں مسلسل اپنے فن میں مبتلا اور فسق و فجور میں ڈوبا ہوا نماز اور عبادت کے بغیر زندگی گزارتا رہا…..پھر اچانک ہماری زندگی میں ایک ہنگامہ برپا ہوا۔ یہ 1988کی بات ہے، میری بیوی نے مجھ سے کہا:"میں اس شخص کے ساتھ ہرگز زندگی نہیں گزارسکتی جو اپنے رب کا وفادار نہیں، جو نماز ادا نہیں کرتا اس کی کمائی حرام ہے جو

 فجر کے وقت گھر آتا ہے میری وہم و گمان میں بھی نہیں آسکتا تھا کہ میری بیوی میرے لیے ایسا سوچ سکتی ہے۔ بہرحال گھر میں لڑائی شروع ہوگئی۔ میں نے اس کی باتیں سنی ان سنی کردیں

"کچھ دن گزرے، ایک دن جب میں گھر میں داخل ہوا تو فجر کی اذان ہورہی تھی۔ شہر کی مساجد میں اذانیں بلند ہورہی تھیں۔ ہر طرف اللہ اکبر۔۔۔اشھد ان لا الہ الا اللہ حی علی الصلاة کی گونج تھی۔ جب سونے کیلئے اپنے کمرے میں جانے لگا تو میری بیوی نے کہا: "آپ مسجد میں نماز کیلئے کیوں نہیں جاتے؟ کیا آپ نے اذان کی آواز نہیں سنی؟"


میری زندگی میں یہ پہلا موقع تھاکہ کسی نے مجھے نماز کیلئے کہا تھا۔ اس لمحے میں نے خود بھی نماز پڑھنے کے بارے میں سوچا میرے جسم میں جھرجھری سی آئی۔ بیوی کی آواز بار بار کانوں میں گونج رہی تھی۔ "اس وقت مسلمان مسجد کی طرف جارہے ہیں۔ آپ کیوں مسجد کا رخ نہیں کرتے؟ یہ رحمن کا بلاوہ ہے۔ یہ مالک الموت کی طرف سے دعوت ہے"اور پھر میرے ذہن 

میں خیر اور شر کی کشمکش ہوئی۔ فطرت کی آواز بلندہوئی: تمہارا نام کتنا خوبصورت ہے…..عبد اللہ…..تم اللہ کے بندے ہو۔ مگر نہیں…..تم تو شیطان کے چیلے بنے ہوئے ہو۔ کبھی تم نے اپنے مالک کے سامنے سر نہیں جھکایا۔ تم کب تک زندہ رہو گے ، کب تک زندگی رہے گی، کب تک جوانی رہے گی؟ میرے سامنے ماضی آگیا…..ضمیر نے ملامت شروع کی…..مگر فوراً کلب کی رعنائیاں، ٹیلی ویژن کی سکرین، اسٹیج، شہرت، عزت…..کیا میں بیوی کی بات مان لوں؟ یہ کام چھوڑ دوں؟ یہ مقام حاصل کرنے کیلئے میں نے بے حد محنت اور جدوجہد کی ہے۔ یہی

 سوچتے سوچتے میں حسب عادت سوگیا"

"شام کے وقت میں نے کپڑے تبدیل کیے۔ کلب جانے کیلئے تیاری کی…..میری بیوی نے میرے کان میں سرگوشی کی، اس کی آنکھوں میں آنسو تھے۔ وہ کہہ رہی تھی: ذرا بیٹھ جائیں…..ذرا میری بات تو سنیں…..کیا ہمارا رازق اللہ نہیں ہے؟ حلال کا ایک لقمہ حرام کے ہزاروں لقموں سے بہتر ہے"

"مجھے ایسا محسوس ہو اکہ بیوی کی آواز…..اس کی گفتگو…..اس کے کلمات…..یقینا درست ہیں۔ ان میں صداقت ہے…..یہ فطرت کی آواز ہیں…..مگر…..میرا فن…..میری آواز…..میری شہرت؟…..میں تیزی سے بھاگا کہیں بیوی کی بات مان نہ لوں"

"راستے میں بیوی کے کلمات میرا پیچھا کر رہے تھے کہ میں نائٹ کلب کے دروازے پر پہنچا۔ اس دوران عشاءکی نما ز کا وقت ہوچکا تھا۔ میرے کانوں میں موذن کی خوبصورت اور دل میں اتر جانے والی آواز گونجی…..حی علی الصلاة…..حی علی الفلاح….. "

"بیوی کی نصیحت یاد آئی…..اللہ کی رحمت جوش میں آگئی۔ فسق و فجور اور کفر کے غبار کی تہ بیٹھنے لگی…..ایمان کی حرارت اور اسلام کی قوت زوردکھانے لگی…..اور پھر میرا رخ نائٹ کلب سے مسجد کی طرف ہوگیا"

"میں مسجد میں داخل ہوا، وضو کیا ۔ جماعت ہورہی تھی، میں نے نماز ادا کی۔ بعض نمازیوں نے مجھے پہچان لیا۔ کوئی ہاتھ ملا رہا ہے، کوئی دور سے سلام کررہا ہے۔ ان کے چہروں پر مسکراہٹ اور میرا چہرہ خوشی سے دمک رہا ہے۔ الحمد اللہ میں نے فطرت کو پالیا ہے"

"کسی نے مجھے صحیح بخاری کا نسخہ تحفے میں دیا۔ یہ اب میرے لیے متاع حیات تھی…..میں اپنی نئی ماڈل کی قیمتی گاڑی میں سوار ہوا۔ اس کا رخ نائٹ کلب کی بجائے گھر کی طرف دیکھا۔ میری بیوی جو مجھے فجر کے وقت گھر آتے دیکھاکرتی تھی…..آج عشاءکے بعد گھر میں دیکھ رہی تھی۔ بیوی کی طرف بڑھا۔ "بیگم…..تمہیں مبارک ہو…..میں نے آج سے گانوں سے توبہ کرلی ہ۔ میں نے فسق وفجور اور لہو و لعب کی زندگی کو تین طلاقیں دے دی ہیں۔ میں نے سچی توبہ کرلی ہے۔ میں الحمد اللہ تائب ہوگیا ہوں۔

"پھر میں نے محسوس کیا گویا میں نے نئی زندگی کا آغاز کیا ہے سب سے پہلا کام وہ اسٹوڈیو، جس کا میں مالک تھا، جس میں گانے ریکارڈ کراتا تھا، جس میں دنیا بھر کی جدید مشینیں تھیں، جن کو دنیا کے کونے کونے سے جمع کرتا رہا تھا…..میں نے اس اسٹوڈیو کو دعوت الی اللہ کیلئے وقف کردیا کہ اب یہاں قرآن پاک کی کیسٹیں، علمائے کرام کی تقاریر اور اسلامی ترانے ریکارڈ ہونگے میں نے قیمتی گاڑی فروخت کردی، خوبصورت محل نما کوٹھی فروخت کردی…..میں ایک اوسط درجے کے مکان میں آگیا۔ اب میرا وقت اپنے گھر میں گزرنےلگا۔ میری ایک ہی تمناتھی…..ایک ہی جستجو…..میں حلقہ قرآن سے وابستہ ہو گیا…..اب مجھے قرآن پاک حفظ کرنے کی خواہش تھی"

1990 میں عبد اللہ نے اپنے وطن کو چھوڑنے کا ارادہ کرلیا اور پھر وہ پہلی مرتبہ اس گھر کی زیارت کیلئے آیا جس کی زیارت اور جس کے گرد چکر لگانے کی تڑپ دنیا کے ہر مسلمان کے دل میں ہوتی ہے۔ وہ مکہ مکرمہ پہنچ گیا، نیک بخت بیوی بھی ہمراہ تھی۔ عمرہ ادا کیا تو اس کے ایمان میں مزید اضافہ ہوگیا۔ مکہ مکرمہ میں بعض اہل خیر کو معلوم ہوا ،وہ اس سے واقف تھے …..اس کی سابقہ زندگی سے…..اس کے ماضی سے ….. اس کی اسلام پر پختگی سے …..اور پھر انہوں نے عبد اللہ کو ہاتھوں ہاتھ لیا، اس کی تکریم کی، اس کی کفالت کی ….. کچھ ہی عرصہ میں اس نے قرآن کے دس پارت حفظ کرلیے۔

اب وہ ایک مبلغ تھا عقیدہ کا، اسلام کا ، قرآن کا، حدیث کا پھر وہ اس دوران میں کئی مرتبہ عمرہ کرنے کیلئے مکہ مکرمہ آیا۔ پھر اس کو اس بلد الحرام میں، مکہ مکرمہ میں، اس مبارک اور مقدس شہر کی مقدس مسجد میں بطور موذن موقع مل گیا۔ عبد اللہ آج بھی مبلغ ہے، وہ موذن ہے اسلام کی آواز کا وہ سعودی عرب میں ہو یا صومال میں، ہر جگہ وہ دعوت الی اللہ کا فریضہ سرانجام دے رہا ہے اور نجانے کتنے ہی گنہگاروں اور خطاکاروں نے اس کے ہاتھ پر توبہ کی ہے اور اپنی زندگیاں قرآن و سنت کے مطابق بنالی ہیں تاکہ وہ بھی حقیقی سعادت سے بہرہ ور ہو سکیں بالکل اسی طرح جس طرح عبد اللہ سعادت حاصل کرچکا ہے۔

اللہ کے رسول کا فرمان بالکل حق ہے:

؛؛؛؛؛؛؛؛؛؛؛؛؛؛؛؛ الدنیا متاع و خیر متاع الدنیا المراة الصالحة ؛؛؛؛؛؛؛؛؛؛؛؛؛؛؛؛

"دنیا ایک پونجی (فائدہ کی چیز) ہے اور دنیا کی بہترین پونجی نیک بیوی ہ

--  

_______ استغفر الله _______

 !...کاش  آزاد  قبیلے  کے  سخنور  ہوتے 
 !..ہم محاذوں پر نہ بکتے تو سکندر ہوتے 
 خود فریبی کے خوابوں میں رہے ہم ورنہ 
 !....اپنی اوقات میں رهتے تو قلندر ہوتے
موج کوثر کی قسم ہم تھے محبت کے والی 
خاک کے در پے نہ جھکتے تو سمندر ہوتے 
آنکھ نی خواب کے لالچ میں خیانت کر لی
! .. ورنہ ہم جاگتی رتوں کے پیامبر ہوتے 

___________________


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--
"  Himmat hay to Paida ker Fardous-e-barein Apna
   Mangi hoi jannat say Dozakh ka Azaab Acha      "  ( Iqbal )


Muhammad Imran Sh.

Faisalabad.


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Virtual University of Pakistan*** IT n CS Blog
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Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 3/31/2012 04:26:00 AM

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