Re: [DIYbio] Paper electrophoresis... super available molecule separation?

Hm, with the right buffering it might be possible to get this to work
with DNA, too. You want a buffer that interferes with the pi-bonding
that attracts DNA to cellulose fibers.

With chemical interactions out of the way, high-grade cellulose looks a
lot like agarose, chemically speaking. In fact, running a "gel" through
a lump of microbial cellulose has been a low-grade item on my to-do list
for a long time. Biggest barrier right now: I killed my kombucha
(G.xylinus) cultures long ago. :P

Would love to know if this would work.

On 20/01/13 00:11, Nathan McCorkle wrote:
> So my thinking is everyone can get paper of some form, but not
> agar/agarose... seems it's not ideal for DNA but it is for amino acids
> and small proteins... it might be useful for DNA or large proteins in
> certain assays though... i.e. yes/no assays or something
>
> here are some things i dug up:
>
> mentions a few paragraphs of history
> https://www.idtdna.com/pages/docs/educational-resources/gel-electrophoresis.pdf
>
> including
> "
> While paper and other solid support materials proved to be an
> advantage over free
> solutions for the electrophoretic analysis of biomolecules, gels were
> adopted later
> because gels not only minimized diffusion better than paper supports
> they actually
> participated in the separation process by interacting with the
> migrating particles.
> "
>
> and referencing this article about Bothrops (pit viper snake) venom,
> but it's in German (I think) and I can't read it (google translate
> doesn't do much either because copy-paste don't keep the sepcial
> characters)
> http://nathanmccorkle.com/pdf/this%20might%20be%20about%20paper%20electrophoresis.pdf
>
> I got thinking because I saw this chromatography paper for $0.65 per
> foot and mentions "widely used for electrophoresis"
> http://store01.prostores.com/servlet/thescienceshop/the-2012/CHROMATOGRAPHY-PAPER-2cm-Wide/Detail
>
>
> thesis on
> "Development of Paper electrophoresis technique for observation of
> microgram quantities of protein" and "electrophoretic and
> ultracentrifugal studies of soluble antigen-antibody complexes as a
> method of determining antibody and antigen valences"
> James T. Bradbury, California Institute of Technology, 1956
> http://thesis.library.caltech.edu/995/1/Bradbury_jt_1956.pdf
>
>

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