Science

Biotin / streptavidin come to mind .... Just at first pass. ....
>matt

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On Oct 31, 2015 3:15 PM, Yuriy <yuriy...@gmail.com> wrote:

Hello everyone.

I'll try to be as brief as possible, given the lack of knowledge on the subject. I don't know where to start. That's why I came here with the question.

I was looking at my drill and it dawned on me that there isn't anything like it, to my knowledge, in molecular biology. It is a tool that alone has no use but given the proper fittings and combination of tool:fittings (adapters), can get the job done. The drill bit end for example, has six sides that accommodate the adjustable chuck. That's what I was looking into for the last few days.

Is there a good, on both transcribed, antibody to antigen fitting to adapt fitting to a molecular tool?

If yes do they have to be on termini? Or can it jet out of aa design via linkers?

I essentially need the tool that will be stable in vivo, while the adapters can undergo a higher turnover under different environmental ques.

Just looking for a good non-bulky-lock and key to be encoded for the tool set.

-Yuriy

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Hello everyone.

I'll try to be as brief as possible, given the lack of knowledge on the subject. I don't know where to start. That's why I came here with the question.

I was looking at my drill and it dawned on me that there isn't anything like it, to my knowledge, in molecular biology. It is a tool that alone has no use but given the proper fittings and combination of tool:fittings (adapters), can get the job done. The drill bit end for example, has six sides that accommodate the adjustable chuck. That's what I was looking into for the last few days.

Is there a good, on both transcribed, antibody to antigen fitting to adapt fitting to a molecular tool?

If yes do they have to be on termini? Or can it jet out of aa design via linkers?

I essentially need the tool that will be stable in vivo, while the adapters can undergo a higher turnover under different environmental ques.

Just looking for a good non-bulky-lock and key to be encoded for the tool set.

-Yuriy

I'm not an expert on reading patents, but it looks like the patent covers the method of the cloning procedure, not the production of SLiCE extract, nor the composition matter of SLiCE extract. Selling the SLiCE mix might not actually violate the patent (that'd be up to the user to do or not do). It also looks like the the patent only covers assembly of fragments that have "20 bp to 52 bp that are homologous". So using 19bp or 53bp homologous regions might be a way around the patent. Maybe a bit less efficient, but it looks like that would still work.

On Fri, Oct 30, 2015 at 3:03 PM Koeng <koen...@gmail.com> wrote:

Yea, my apologies for the non-controlled experiment, I didn't set it up to be very controlled because I didn't think I'd get that good of efficiency. I will be running a few more in the next few weeks so I will post the results. 

Unfortunately enough, it appears that SLiCE is patented ( http://www.google.com/patents/US20130045508 ), and the company that owns the patent charges an arm and a leg ( http://slicecloning.com/SLiCE.html , more than HiFi mix per reaction). I guess the ODIN can't sell the mix, but possibly sell a kit to make it yourself.

-Koeng

On Thursday, October 29, 2015 at 3:12:01 PM UTC-7, Josiah Zayner wrote:

This is pretty cool. If people try and repeat these experiments can they post detailed details(heh) of what they did so others can follow suite.

Also, it doesn't seem anyone is running the control of everything but the SLiCE mixture. If you are running an experiment can you do that and let us know how that goes?

Thanks,

     Josiah

On Thu, Oct 29, 2015 at 2:44 PM, Koeng <koen...@gmail.com> wrote:

I tried out my homemade SLiCE mix. I put 100ng of both insert and vector in for a 2 piece assembly, for 30 minutes. I compared it to some homemade Gibson mix.

On my plates, I got 13 colonies on the SLiCE plate and 42 colonies on the Gibson. I am extremely impressed with 1/3 considering just the simple strain and simple lysis procedure.

-Koeng

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