[DIYbio] Re: DIYBio Singapore?

The sustainable living lab is currently active, engaging the maker community to build up their DIY BioLab every saturday on a volunteer basis. see https://www.facebook.com/groups/themakercircle/files/ for progress and join in if you can
//saad

On Sunday, March 12, 2017 at 7:00:48 PM UTC+8, John Cumbers wrote:
Hi all, 

Is anyone active in DIYBio in Singapore?

Regards
John

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[DIYbio] Re: dNTPs from cells

"A procedure for rapid extraction and high-pressure liquid chromatographic separation of the nucleotides and other small molecules from bacterial cells" 

They use forimc acid to extract the small molecules. 


However, there is a lot of dNDPs and dNMPs as well as NTPs, NDPs and NMPs present. Maybe high ammounts of AMP inhibits polymerase by indicating that there's little energy left 

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[DIYbio] dNTPs from cells

Hi guys,

For what I know TAQ polymerase and T4 Ligase is pretty stable.

dNTPs not so much, although alledgedly without any freeze thaw cycle they retain 90% activity after 7 weeks of storage at room temperature.

Now it should be possible to take a crude bacterial lysate (must be DNAse free) instead of dNTPs. Will look into small molecule extraction protocols tomorrow.

Anyone ever heard of this?

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[DIYbio] Diy biohack article.

http://makezine.com/2017/03/23/biohacking-projects/

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[DIYbio] Re: Molecular Biologist looking for collaborator for a Metabolic Modelling Project

Hi there,

I'm another molecular biologist / bioinformatician also in Edinburgh. I know little about modelling per se, but I'm teaching python to biologists and I'm an avid DIYbio person, so I am certain we can come up with something if you need help. If you want to meet up just let me know, I'm at King's Buildings.

Cheers,
Mate


On Friday, 24 March 2017 18:35:14 UTC, Paolo Marangio wrote:
I am a Molecular Biology Resarch Assistant based in Edinburgh and I recently came across an interesting paper where they used modelling for coming up with deletion strategies that allow increased production of cis-aconitic acid in E. coli (you can read all about it here: https://www.ncbi.nlm.nih.gov/pubmed/27269589). SInce I found this approach particularly powerful, I decided to do some modelling out of interest. I am planning on using the python package Cobrapy for this (http://opencobra.github.io/cobrapy/) as I don't know how to use MATLAB. After I will have identified some deletion strategies, I could test them directly as I have access to a lab. I was therefore wondering if there is anybody on here who has experience with this package or constraint-based modelling in general and would like to collaborate in this small project. 

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Re: [DIYbio] Molecular Biologist looking for collaborator for a Metabolic Modelling Project

There's a decently active group that's dedicated to cobrapy:


They might have a larger pool of experienced users.

Otherwise I've used cobrapy a few times before, and I'm happy to chat a bit more with what you're interested in doing.

Francis

On Fri, Mar 24, 2017 at 2:30 PM, Paolo Marangio <marangiopaolo@gmail.com> wrote:
I am a Molecular Biology Resarch Assistant based in Edinburgh and I recently came across an interesting paper where they used modelling for coming up with deletion strategies that allow increased production of cis-aconitic acid in E. coli (you can read all about it here: https://www.ncbi.nlm.nih.gov/pubmed/27269589). SInce I found this approach particularly powerful, I decided to do some modelling out of interest. I am planning on using the python package Cobrapy for this (http://opencobra.github.io/cobrapy/) as I don't know how to use MATLAB. After I will have identified some deletion strategies, I could test them directly as I have access to a lab. I was therefore wondering if there is anybody on here who has experience with this package or constraint-based modelling in general and would like to collaborate in this small project. 

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[DIYbio] Molecular Biologist looking for collaborator for a Metabolic Modelling Project

I am a Molecular Biology Resarch Assistant based in Edinburgh and I recently came across an interesting paper where they used modelling for coming up with deletion strategies that allow increased production of cis-aconitic acid in E. coli (you can read all about it here: https://www.ncbi.nlm.nih.gov/pubmed/27269589). SInce I found this approach particularly powerful, I decided to do some modelling out of interest. I am planning on using the python package Cobrapy for this (http://opencobra.github.io/cobrapy/) as I don't know how to use MATLAB. After I will have identified some deletion strategies, I could test them directly as I have access to a lab. I was therefore wondering if there is anybody on here who has experience with this package or constraint-based modelling in general and would like to collaborate in this small project. 

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[DIYbio] "Home brewing [beer] automation with internet processor board" tech video

This came my way via an engineering group. I didnt check it out. Some
of you may be interested regarding current hardware
options/possibilities for lab automation projects.

slides - http://www.slideshare.net/RobertWolff5/homebrewing-and-automation-with-96-boards

--quote--
This week
OpenHours Episode #45 – This week on 96Boards OpenHours, we will be
speaking with Linaro's very own Ricardo Salveti. Ricardo has a fun
demo planned for us! Does anyone like beer? What about beer for
breakfast? In this exciting installment, Ricardo will show us how to
use 96Boards to set up your very own home brew system. Join us with
your regular cup of coffee or… Your favorite beer! ...
Don't forget to join us in our new OpenHours IRC channel: #OpenHours & #96Boards
Find out more about Testing Days in their IRC channel: #ubuntu-on-air

....

https://www.youtube.com/watch?list=PL-NF6S9MM_W1QBjUc2B5Pg502bz7qslxk&v=dFh5p-LHfNI




--
## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
########################

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[DIYbio] Re: Southwestern PA

https://www.youtube.com/watch?v=Dw5MJHh86Fc

https://www.youtube.com/watch?v=iXuI4Q49kxY

http://diyhpl.us/~bryan/papers2/diybio/r%C3%BCdiger-trojok-gene-gun.pdf


Doesn't look too difficult 







On Saturday, March 11, 2017 at 5:38:41 PM UTC+1, Mega [Andreas Stuermer] wrote:
There are people who have successfully built gene guns for a few dozens of bucks. Avoids agrobacterium regulation

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Re: [DIYbio] Re: Southwestern PA

On Thu, Mar 23, 2017 at 3:34 PM, Sean Donahue <sean.donahue5311@gmail.com> wrote:
My apologies for the lack of a timely response. 


couldn't resist :)

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[DIYbio] Re: Southwestern PA

My apologies for the lack of a timely response.  I've had my hands full lately, but I should have more free time in the near future for side projects.  If there's a way to avoid the agrobacterium related red tape, I'm open to it.  I'd looked into a DIY gene gun several months ago, but hadn't found much.  Perhaps I'll look again.

On Saturday, March 11, 2017 at 11:38:41 AM UTC-5, Mega [Andreas Stuermer] wrote:
There are people who have successfully built gene guns for a few dozens of bucks. Avoids agrobacterium regulation

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[DIYbio] File format detection and parser validation

Does anyone know of a tool for detecting file formats for common biology files, e.g. FASTA, FASTQ, GenBank, SBOL, AB1, etc.

The *nix file command / libmagic does a terrible job of this.

I'm also looking for a library of samples that showcase the diversity of formats and *ahem* variants of those formats for the purpose of ensuring that parsers don't fail on edge cases.

--
marc/juul

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Required || Java Developer || Sunnyvale, CA ||

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Java Developer

Sunnyvale, CA

6+ Months

Skype Interview

 

 

·         Please find the job description of the position.

·         Fluent in Java 6, and at least working knowledge of Java 8

·         Strong Object Oriented Design background

·         Comfortable with Spring

·         Experience with Cassandra

·         Basic knowledge of Kafka

·         Understanding of Reactive Functional Programming (preferred)

·         Detail-oriented, self-starter, strong written and oral communication skills

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home

cid:image009.jpg@01D02C05.4FFC5560

We Find Hidden Talent

 

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Required || Big Data/Kafka LEAD/Architect || Sunnyvale, CA

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

 

Kafka Expert Developer

Sunnyvale, CA

6+ Months


Primary skill: Kafka
Kafka - architecture, stabilization. Must have 2-3 years Kafka experience.

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home

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We Find Hidden Talent

 

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Re: [DIYbio] How big is the DIYBio community? #OPENBIOTECH vlog series

I guess at this point it depends what "activity threshold" you would put for the definition of biohacker, which is an interesting question per se.

On Saturday, 18 March 2017 16:02:31 UTC+1, Pieter wrote:
Thanks for sharing your estimates. I am also unsure about the numbers. 5000 seemed like a decent estimate at first. Some, not all, growers are doing pretty advanced molecular stuff. Also, I remember deriving my first design of a laminar flow hood from a description on a orchid amateur website: http://www.orchideenvermehrung.at/english/lfh/use.htm

Anyhow, I just returned from Warsaw and Bucharest where I met with people active in DIYBio stuff around there. Even though these people never popup on the mail lists it is pretty cool stuff they are up to. I will show the footage of a Warsaw basement lab in the next vlog

On Saturday, 11 March 2017 06:29:27 UTC+1, Marc Dusseiller wrote:
interesting discussion...

from my side, having visited many places that are doings stuff around DIYbio, i would also reduce the numbers a lot.
for now it doesnt make sense to include the foodies and growers, cos your policy work is more specific i think.

if you also not count a random person that once joined a "strawberry DNA extraction" at a booth in a makerfaire...
talking for europe i would but the number even below 500.

looking at who is active in the various maillists... i' even go down to 100.
sorry pieter... why you even want to put a number like 5000 in Europe?

so, globally we can still stand for something like 2000?

greets,
marc

On Saturday, March 11, 2017 at 12:35:24 AM UTC+8, Pieter wrote:
The thing with statistics is of course the problem of scope definition. I'd very interested in setting up a new survey!

@Sebastian, once we start including the home growers in the definition, 5.000 is all of sudden a very conservative estimate. On itself http://www.wietforum.nl has already 32.000 members. Although there is not much molecular stuff going on over there. The bulb flower breeding community is more active on that front.

On Friday, 3 March 2017 06:30:15 UTC+1, Sebastian wrote:
I wonder how many plant people are on the list. Show of hands? 

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC


On Mar 2, 2017, at 11:47 AM, Pieter Waag <pie...@waag.org> wrote:

We just published the third episode of our #OPENBIOTECH vlog series: https://www.youtube.com/watch?v=WpVp9YH8Znc

This time I am trying to do a sensible estimate of the size of the DIYBio community. I really wonder what your best estimate would be. 

There are nearly 6000 people on this mailing list, but are these really all active DIYBiologists? The mailing list of our lab in Amsterdam alone already contains over 1000 people, so I guess that's not a great way to make an estimate.

On the "local" lab page of the diybio.org site there are about 100 labs listed. Does every lab have at least 10, 50 or perhaps 100 active members?

And how about the DIY neuro/brain, (animal) breeders, nutrition/foodhackers, grinders/bodyhackers (just check #biohacking on instagram)? Do you think these should be considered as part of the DIYBio movement as well? The vlog also contains an interview with Christine Marizzi of the DNA Barcoding project. In think it is fair to include their participants as well. Do you agree?

For the vlog I assumed it was safe to say there are about 5,000 people in Europe involved in this scene. That's my best guess for now. 500 feels like a too small number to me, while 50,000 is way too much. So I suppose the order of magnitude is right. 

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Re: [DIYbio] Re: Safely disposing of old cultures?

On Tue, Mar 21, 2017 at 1:54 PM, Mega [Andreas Stuermer]
<masterstorm123@gmail.com> wrote:
> If you heat kill it in a pressure cooker at 121°C; 20 mins that would be
> good practice though

That's what we did at school with an autoclave.

I won't cook such things in my main for-food kitchen though. Just my
paranoia about volatilizing weird stuff that might have grown.
Also, around here I feel like pressure-cooking outdoors on the porch
might disturb the neighbors. So I will need to do it during the
daytime, at least, if I don't think of a better solution soon.

I just bought a CO2 laser cutter though, which also needs exhaust, and
my spin-coater could use exhaust too. So I might just be on the
lookout for sheet metal and try building something of my own, for the
garage or something.

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[DIYbio] Re: Safely disposing of old cultures?

AFAIK all trash (Restmüll - residual trash) in my country is brunt to get energetic value. 

We have 4 containers - paper trash, plastic trash and bio trash and residaul trash (everything else). I would assume non-modified non-pathogenous coli should be fine to go into trash. People trow molding bread in there and molds may actually poison you with mycotoxins and allergens etc (http://news.nationalgeographic.com/news/2005/05/0506_050506_mummycurse.html). 

If you heat kill it in a pressure cooker at 121°C; 20 mins that would be good practice though
 




On Saturday, March 4, 2017 at 6:49:49 PM UTC+1, MC wrote:
For those of us without access to biohazard waste receptacles, how do you safely dispose of old cultures? For example, non-pathogenic E. coli (BL21, DH10b). Is it safe enough to just toss in the trash? Or what is an effective way to ensure sterilization (in non-autoclaveable plates) before heading to the trash?

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[DIYbio] Re: Safely disposing of old cultures?



On Saturday, March 4, 2017 at 6:49:49 PM UTC+1, MC wrote:
For those of us without access to biohazard waste receptacles, how do you safely dispose of old cultures? For example, non-pathogenic E. coli (BL21, DH10b). Is it safe enough to just toss in the trash? Or what is an effective way to ensure sterilization (in non-autoclaveable plates) before heading to the trash?

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Required || Kafka Expert || Sunnyvale, CA

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

 

Kafka Expert

Sunnyvale, CA

6+ Months


Primary skill: Kafka
Kafka - architecture, stabilization. Must have 2-3 years Kafka experience.

 

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home

cid:image009.jpg@01D02C05.4FFC5560

We Find Hidden Talent

 

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Required || Tableau Administrator || San Jose, CA

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Tableau Administrator with Strong SQL experience

 San Jose, CA

6+ Months

Hangout Interview

Minimum relevant experience 5+ years of experience in Business Intelligence and Reporting/Visualization tool administration (preferably, Tableau)

                                                                                               

Description:

1.            Gather requirements, design, build, test and deploy Tableau reports

2.            Provide hands-on Tableau production support. Identify and research problem trends and work with internal or external subject matter experts to correct and proactively prevent further problems by recommending and/or implementing bug-fixes, application enhancements, customer training, business process changes or other measures

3.            Administer and support a multi-tier Tableau infrastructure (Tableau Server, Server Arch, Server side components) including upgrade analysis and planning, security administration, release management, troubleshooting, performance tuning, and general systems maintenance

4.            Experiment with new features and enhancements released by Tableau, and bring them into the platform & organization.

 

Technical Skills:

             Tableau Desktop, building and publishing dashboards

             Tableau Server

             SQL skills

             Data warehousing, data modelling

             Problem-solving, troubleshooting skills

             Requirements gathering, documenting design/processes

             Big Data

 

Soft Skills:

             Excellent communication skills: Role involves interfacing with internal customers, external software vendors

             Organization skills: Ability to keep up with high volume of tickets/requests

             Self-starter: Role requires proactively initiating tasks. Ex: planning for upgrades, testing beta versions, etc.

             Industry knowledge: Ability to keep up with upcoming releases of related software, assess impact on platform infrastructure/user-base

 

 

Best Regards,

 

Arpit Arora

Pyramid Consulting, Inc.


Desk Phone: 770-255-3177 Ext 827  Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Webhttp://www.pyramidci.com/staffing-home


We Find Hidden Talent

 

 

 

 

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[DIYbio] PCR machines super cheap

$50- MJR Minicycler.  Some of the plastics are broken off of it, but it works fine as long as the saftey switch is depressed.  Comes with heated lid attachment, but I think this has the wrong block for it.  Comes with it anyway, if should be easy to heat and just stick on top of the block if you are crafty.

$50- MJR PTC-100 cycler.  Good condition, no heated lid.

You pay shipping!  Happy to ship international.

If these don't go soon they will be sacrificed to the teardown gods.

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Re: [DIYbio] Anyone ever see electrophoresis where the gel was rolled?


On Mar 16, 2017 6:58 AM, "Kermit Henson" <kermitlab@gmail.com> wrote:
>
> What happens if the roll is not perfect parallel to the support? when you run a polyacrylamide gel, is easy to get distorted lanes
>

Hmm, good point. I guess you would need to ensure the gel was straight, at least the center where you'd want to load the DNA.

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Re: [DIYbio] Re: Possible filters for working with fluorescence



On Sunday, March 19, 2017 at 10:31:26 AM UTC-7, John Griessen wrote:
On 03/18/2017 11:01 PM, John Ladasky wrote:
>  To do this kind of work well, you typically want < 0.001 % transmission (10^-5) of the excitation light.  You can only achieve
> this with the fancy interference filters made of many layers of coatings.  They typically cost a few hundred dollars each, though
> some very popular wavelengths can go for less.

I used to work on a product with these kind made of furnace processed silicon/glass/metals sputtered on in high vacuum.

Won't it be nice when some self assembling nanomaterial can do this kind of light interference filtering?

Hey, you just gave me an idea for a project...  :^) 

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Re: [DIYbio] Re: Possible filters for working with fluorescence

On 03/18/2017 11:01 PM, John Ladasky wrote:
> To do this kind of work well, you typically want < 0.001 % transmission (10^-5)of the excitation light. You can only achieve
> this with the fancy interference filters made of many layers of coatings. They typically cost a few hundred dollars each, though
> some very popular wavelengths can go for less.

I used to work on a product with these kind made of furnace processed silicon/glass/metals sputtered on in high vacuum.

Won't it be nice when some self assembling nanomaterial can do this kind of light interference filtering?

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Re: [DIYbio] EU DIYBio beyond hope, hype and horror - revisited

But true that to assembler

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Re: [DIYbio] EU DIYBio beyond hope, hype and horror - revisited

I dont know how you are doing it but most people don't use dangerous chemicals or dangerous GMOs. S1 is perfectly safe, even the law admits.

Only very few experiments really need radiolabelled stuff

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[DIYbio] Re: Possible filters for working with fluorescence



On Friday, March 17, 2017 at 3:26:51 PM UTC-7, lisa wrote:
I just came across a possible source of filters for working with
fluorescent dyes and such - Rosco E-Colour filters. They're made for
photography and they're dirt cheap, but what I found really neat is that
there's a sampler of all 180 colours that you can buy for 3,50 Euros,
and which features transmission curves - I've often had trouble coming
by this information when digging around for cheap alternatives to
high-end filters. See attached photo.

Has anyone worked with these, by chance?

Not this specific brand, but I am familiar with things like them.  Gel filters and colored-glass filters typically transmit around 1% of the light at wavelengths that are "rejected."  Is that good enough?  It depends what you need them to do.  If you are doing fluorescence work, you have a bright illumination source, and much dimmer fluorescence of a different color.

Say you were working with a DNA - ethidium bromide gel, on a blue, violet or a near-UV transmission table.  A 540 nm long-pass piece of glass can improve the contrast for your eyes a bit.  If you are working with a black-and-white camera and want to take a picture of the gel, it can also help.  How much depends how sensitive the camera is to the excitation wavelength. 

If you are doing cellular fluorescence microscopy, you need much better sensitivity.  You usually use a laser excitation source and focus it with a lens.  The scattered light off the cell is strong, and the fluorescence is weak.  You hope to achieve quantitative results -- in other words, where there's no fluorescence, you want the pixel to be as close to background levels as possible.  To do this kind of work well, you typically want < 0.001 % transmission (10^-5)of the excitation light.  You can only achieve this with the fancy interference filters made of many layers of coatings.  They typically cost a few hundred dollars each, though some very popular wavelengths can go for less.

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Re: [DIYbio] EU DIYBio beyond hope, hype and horror - revisited

amen. better tools would help a lot. realistically how many people would use computers if we all need to understand assembler or machine language. we need better tools (high level) where people can do testing/playing just with higher order stuff, like MB kits but for masses. How to do that w/o dangerous chemicals/modified organisms, I don't know but analytics is a first step that is for sure. 

On Saturday, March 18, 2017 at 10:41:08 AM UTC-5, Abizar Lakdawalla wrote:
I apologize upfront about the provocative language below ...
Unfortunately, some of the diybio is driven more by hype than any expectation of reality. 
It is a derivative of the philosophers dilemma - your confidence in achieving something is inversely proportional to your knowledge about the subject (aka the less you know the more cocky you are). That is why many enter the DIYbio community with great excitement but then lose steam when they cannot make the rapid progress they expected. Bio, DIY- or otherwise, is a grind. It's that last 20% that takes 80% of the effort.
The challenge to expanding the DIY community is to keep the excitement going but tie them to projects that have a defined end that can be achieved in a reasonable period of time. One example, developing assays to survey antibiotics in our bodies through urine or saliva, involving a global community to do the tests on themselves and creating a google map with the incidence of antibiotics in our bodies.

On Sat, Mar 18, 2017 at 8:19 AM, Pieter <pieterva...@gmail.com> wrote:
Do you remember the article "European do-it-yourself (DIY) biology: beyond hope, hype and horror" from early 2014? http://onlinelibrary.wiley.com/doi/10.1002/bies.201300149/full

"We found that all groups are driven by a core leadership of (semi-)professional people who struggle with finding lab space and equipment. Regulations on genetic modification limit what groups can do. Differences between Europe and the US are found in the distinct regulatory environments and the European emphasis on bio-art. We conclude that DIYBio Europe has so far been a responsible and transparent citizen science movement with a solid user base that will continue to grow irrespective of media attention."

For the next episode of the #OPENBIOTECH youtube series I met with co-author Günter Seyfried to discuss the most remarkable differences between now and the situation some years ago.
https://www.youtube.com/watch?v=dCVbF0ZxMC0

The conclusion that DIYBio is here to stay still holds, and it is interesting to analyse what has changed. At the time Cathal was the only with a GMO permit for his lab, now there are more but not many. The groups have developed into several different directions, resembling hacker spaces, co-working spaces or are part of larger institutions. The movement has functioned as a test-bed for startups. Collaborations with companies have also occurred (f.e. BiologiGaragen - Novozymes, La Paillasse - Roche).

Günter and I also discussed the influence of governmental support in the growth of DIYBio activities. To what extend has that made a difference?

In the same vlog you can also see a meetup with two Belgium biohackers in Brussels, with their own opinions and expectations.

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Re: [DIYbio] EU DIYBio beyond hope, hype and horror - revisited

amen. better tools would help a lot. realistically how many people would use computers if we all need to understand assembler or machine language. we need better tools (high level) where people can do testing/playing just with higher order stuff, like MB kits but for masses. How to do that w/o dangerous chemicals/modified organisms, I don't know but analytics is a first step that is for sure. 

On Saturday, March 18, 2017 at 10:41:08 AM UTC-5, Abizar Lakdawalla wrote:
I apologize upfront about the provocative language below ...
Unfortunately, some of the diybio is driven more by hype than any expectation of reality. 
It is a derivative of the philosophers dilemma - your confidence in achieving something is inversely proportional to your knowledge about the subject (aka the less you know the more cocky you are). That is why many enter the DIYbio community with great excitement but then lose steam when they cannot make the rapid progress they expected. Bio, DIY- or otherwise, is a grind. It's that last 20% that takes 80% of the effort.
The challenge to expanding the DIY community is to keep the excitement going but tie them to projects that have a defined end that can be achieved in a reasonable period of time. One example, developing assays to survey antibiotics in our bodies through urine or saliva, involving a global community to do the tests on themselves and creating a google map with the incidence of antibiotics in our bodies.

On Sat, Mar 18, 2017 at 8:19 AM, Pieter <pieterva...@gmail.com> wrote:
Do you remember the article "European do-it-yourself (DIY) biology: beyond hope, hype and horror" from early 2014? http://onlinelibrary.wiley.com/doi/10.1002/bies.201300149/full

"We found that all groups are driven by a core leadership of (semi-)professional people who struggle with finding lab space and equipment. Regulations on genetic modification limit what groups can do. Differences between Europe and the US are found in the distinct regulatory environments and the European emphasis on bio-art. We conclude that DIYBio Europe has so far been a responsible and transparent citizen science movement with a solid user base that will continue to grow irrespective of media attention."

For the next episode of the #OPENBIOTECH youtube series I met with co-author Günter Seyfried to discuss the most remarkable differences between now and the situation some years ago.
https://www.youtube.com/watch?v=dCVbF0ZxMC0

The conclusion that DIYBio is here to stay still holds, and it is interesting to analyse what has changed. At the time Cathal was the only with a GMO permit for his lab, now there are more but not many. The groups have developed into several different directions, resembling hacker spaces, co-working spaces or are part of larger institutions. The movement has functioned as a test-bed for startups. Collaborations with companies have also occurred (f.e. BiologiGaragen - Novozymes, La Paillasse - Roche).

Günter and I also discussed the influence of governmental support in the growth of DIYBio activities. To what extend has that made a difference?

In the same vlog you can also see a meetup with two Belgium biohackers in Brussels, with their own opinions and expectations.

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