tag:blogger.com,1999:blog-89085317476713589802024-03-13T23:17:10.066-07:00ScienceSciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.comBlogger32459125tag:blogger.com,1999:blog-8908531747671358980.post-63329330409009125942024-02-29T11:45:00.001-08:002024-02-29T11:45:40.637-08:00Re: [DIYbio] Therapeutic plasma replacement, infusion and exchange<div dir="ltr">The effects of rapamycin are... complex. Its primary use (right now) is as an immune system suppressant for transplant patients. Anyone using it for anti-aging needs to be very careful about bacterial infections. It should go without saying that this is not medical advice.<div><br></div><div>I don't think the mechanism by which rapamycin combats the effects of aging are understood. Of course, aging itself is not well understood.</div><div><br></div><div>Here are some papers:</div><div><br></div><div>Rapamycin for longevity: opinion article<br></div><div><a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814615/">https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6814615/</a><br></div><div><br></div><div>Rapamycin, the only drug that has been consistently demonstrated to increase mammalian longevity. An update<br></div><div><a href="https://www.sciencedirect.com/science/article/pii/S0531556523000876">https://www.sciencedirect.com/science/article/pii/S0531556523000876</a><br></div><div><br></div><div>Cheers</div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 29, 2024 at 12:24 PM Dakota Hamill <<a href="mailto:dkotes@gmail.com">dkotes@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr">ooo I love the story of Rapamycin and always start any talks around natural products with it. In 1972 a group from Wyeth Brazil went to Easter Island and took soil samples from the edge of the extinct volcano there. Originally Streptomyces hygroscopicus had a bio-active being pursued for anti-fungal properties. Turns out it was an amazing immune suppressant (I do not know how this was tested). Rapamycin, named after the native people's name for the island, Rapa Nui, is still used today to coat heart stents and after organ transplantation. <div><br></div><div>The molecule has 15 chiral centers, and if you do 2^15 there are 32,768 unique isomers. And a little microbe, eating decaying organic matter, with some oxygen, beautifully synthesizes an amazingly active molecule which is a multi billion dollar life saving medicine. </div><div><br></div><div>I've never heard about its anti aging uses, would like to hear more. </div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 29, 2024 at 12:49 PM Daniel C. <<a href="mailto:dcrookston@gmail.com" target="_blank">dcrookston@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr">I am very interested in this list not being spammed with advertisements for quackery.<div><br></div><div>I especially like how you don't talk about rapamycin, the most effective anti-aging drug currently available, which is a generic medication and therefore not profitable.</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 29, 2024 at 7:41 AM jadams <a href="http://grg.org" target="_blank">grg.org</a> <<a href="mailto:jadams@grg.org" target="_blank">jadams@grg.org</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div> <div lang="EN-US"> <div> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">Members,<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal">My name's Johnny Adams. <u></u><u></u></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">I recently moved to Florida to be active in two initiatives that I believe have the greatest potential for biological age management and reversal:<u></u><u></u></span></p> <ul style="margin-top:0in" type="disc"> <li style="margin-left:0in"><span style="font-family:Arial,sans-serif">Yamanaka/OSK gene therapy to reset the epigenome. A therapy is a few years away.<u></u><u></u></span></li><li style="margin-left:0in"><span style="font-family:Arial,sans-serif">A plasma project involving therapeutic plasma replacement, infusion and exchange<u></u><u></u></span></li></ul> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">It will initially be available in Florida, then in other parts of the country.<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">More information:<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><a href="https://www.aginginterventionfoundation.org/JohnnyAdamsPlasmaProjectSummary.pdf" target="_blank">https://www.aginginterventionfoundation.org/JohnnyAdamsPlasmaProjectSummary.pdf</a><u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">Please contact me if you have any interest.<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">Johnny<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">1-949-922-9786 cell<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><a href="mailto:JAdams@AgingInterventionFoundation.org" target="_blank">JAdams@AgingInterventionFoundation.org</a><u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:"Times New Roman",serif"><u></u> <u></u></span></p> </div> </div> <p></p> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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In 1972 a group from Wyeth Brazil went to Easter Island and took soil samples from the edge of the extinct volcano there. Originally Streptomyces hygroscopicus had a bio-active being pursued for anti-fungal properties. Turns out it was an amazing immune suppressant (I do not know how this was tested). Rapamycin, named after the native people's name for the island, Rapa Nui, is still used today to coat heart stents and after organ transplantation. <div><br></div><div>The molecule has 15 chiral centers, and if you do 2^15 there are 32,768 unique isomers. And a little microbe, eating decaying organic matter, with some oxygen, beautifully synthesizes an amazingly active molecule which is a multi billion dollar life saving medicine. </div><div><br></div><div>I've never heard about its anti aging uses, would like to hear more. </div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 29, 2024 at 12:49 PM Daniel C. <<a href="mailto:dcrookston@gmail.com">dcrookston@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr">I am very interested in this list not being spammed with advertisements for quackery.<div><br></div><div>I especially like how you don't talk about rapamycin, the most effective anti-aging drug currently available, which is a generic medication and therefore not profitable.</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 29, 2024 at 7:41 AM jadams <a href="http://grg.org" target="_blank">grg.org</a> <<a href="mailto:jadams@grg.org" target="_blank">jadams@grg.org</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div> <div lang="EN-US"> <div> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">Members,<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal">My name's Johnny Adams. <u></u><u></u></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">I recently moved to Florida to be active in two initiatives that I believe have the greatest potential for biological age management and reversal:<u></u><u></u></span></p> <ul style="margin-top:0in" type="disc"> <li style="margin-left:0in"><span style="font-family:Arial,sans-serif">Yamanaka/OSK gene therapy to reset the epigenome. A therapy is a few years away.<u></u><u></u></span></li><li style="margin-left:0in"><span style="font-family:Arial,sans-serif">A plasma project involving therapeutic plasma replacement, infusion and exchange<u></u><u></u></span></li></ul> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">It will initially be available in Florida, then in other parts of the country.<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">More information:<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><a href="https://www.aginginterventionfoundation.org/JohnnyAdamsPlasmaProjectSummary.pdf" target="_blank">https://www.aginginterventionfoundation.org/JohnnyAdamsPlasmaProjectSummary.pdf</a><u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">Please contact me if you have any interest.<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">Johnny<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">1-949-922-9786 cell<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><a href="mailto:JAdams@AgingInterventionFoundation.org" target="_blank">JAdams@AgingInterventionFoundation.org</a><u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:"Times New Roman",serif"><u></u> <u></u></span></p> </div> </div> <p></p> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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A therapy is a few years away.<u></u><u></u></span></li><li class="m_7764732636506848372MsoListParagraph" style="margin-left:0in"><span style="font-family:Arial,sans-serif">A plasma project involving therapeutic plasma replacement, infusion and exchange<u></u><u></u></span></li></ul> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">It will initially be available in Florida, then in other parts of the country.<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">More information:<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><a href="https://www.aginginterventionfoundation.org/JohnnyAdamsPlasmaProjectSummary.pdf" target="_blank">https://www.aginginterventionfoundation.org/JohnnyAdamsPlasmaProjectSummary.pdf</a><u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">Please contact me if you have any interest.<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><u></u> <u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">Johnny<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif">1-949-922-9786 cell<u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:Arial,sans-serif"><a href="mailto:JAdams@AgingInterventionFoundation.org" target="_blank">JAdams@AgingInterventionFoundation.org</a><u></u><u></u></span></p> <p class="MsoNormal"><span style="font-family:"Times New Roman",serif"><u></u> <u></u></span></p> </div> </div> <p></p> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" target="_blank">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" target="_blank">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CH3PR10MB7531C67D401BE911E4BD07A2CC5F2%40CH3PR10MB7531.namprd10.prod.outlook.com?utm_medium=email&utm_source=footer" target="_blank">https://groups.google.com/d/msgid/diybio/CH3PR10MB7531C67D401BE911E4BD07A2CC5F2%40CH3PR10MB7531.namprd10.prod.outlook.com</a>.<br> </div></blockquote></div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CAGjqrShp547uxrJH5he9jeuJAA3HAQ5nsmK7ev38qn8KU3dgmQ%40mail.gmail.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/CAGjqrShp547uxrJH5he9jeuJAA3HAQ5nsmK7ev38qn8KU3dgmQ%40mail.gmail.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-12559029350157341252024-02-29T08:17:00.001-08:002024-02-29T08:17:45.404-08:00Re: [DIYbio] Re: DIYbio - Week 3 of Jan 2024 notes poked up to this thing<div dir="ltr"><div dir="ltr">Thanks Andrew! Didn't know about the discord. That seems helpful!</div><br><img width="0" height="0" class="mailtrack-img" alt="" style="display:flex" src="https://mailtrack.io/trace/mail/9a6e47bcd9aa234b8e0ffefce7f2c8a1e36e3cce.png?u=2280817"><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Feb 29, 2024 at 4:53 AM Andrew Gray <<a href="mailto:andrew.gray.d2@gmail.com">andrew.gray.d2@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Hans, there are a few out there. r/DIYbio (stay away from r/biohackers, it's a bit crazy) the fb group biohacking & genetic engineering, discord SciHouse (which hosted al #DIYbio on twitter. <br><br><div><br></div><div class="gmail_quote"><div dir="auto" class="gmail_attr">On Thursday, February 29, 2024 at 3:38:23 AM UTC+11 Hans Wilms wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr"><div dir="ltr">Does anyone know where these DIY groups are in the Facebook, Reddit, Discords space? I feel like DIYbio has mostly died off compared to what it was in 2010s, but I'm hoping I'm just out of touch.</div><br><img width="0" height="0" alt="" style="display: flex;" src="data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7"><div class="gmail_quote"></div></div><div dir="ltr"><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Feb 28, 2024 at 11:01 AM Dan Kolis <<a rel="nofollow">dank...@gmail.com</a>> wrote:<br></div></div></div><div dir="ltr"><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><br><br><div><blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div><blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><br>The VC world is plenty happy to convert nutrients to H20 + Urea on a huge scale. I mean, 'hunt the unicorn', then wait for layoffs due to nothing usable seems like a common plan.</blockquote></div></blockquote><div><br> Again in the investment lingo; it is just 'widows and orphans' or do institutions fund endless go nowhere biotech, or what ? I don't know, I have tried to find out, and I am a little interested.<br><br>Like here in Toronto, the teachers Union has thrown in on Quantum computers FOR LIFE SCI, and the Fed and province peeled off millions for little analog computers that do exactly nothing, all blessed with urges based on Stat trek. I mean, That twisty thing on the screen, is that REAL DNA ?". How hard could this be ? Only took 45 seconds for Warf and some hottie to make a cure via an air blown med. Works first time perfectly, before the next commercial !<br><br>Ok. DIY or institutional, non-profit or whatever. Doing what took mother nature 100K years to do before a patent runs out, is not too easy.<br><br>Scale up a hobby, scale down a unicorn. Not so easy. When one atom in a molecule changes hugely what happens, its pretty tough stuff to tame.<br><br>regs,</div><div>Daniel B. Kolis</div><div><br></div><div><br></div><div><br><br><br></div></div> <p></p></blockquote></div></div><div dir="ltr"><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to <a rel="nofollow">diy...@googlegroups.com</a>. To unsubscribe from this group, send email to <a rel="nofollow">diybio+un...@googlegroups.com</a>. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" rel="nofollow" target="_blank">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" rel="nofollow" target="_blank">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a rel="nofollow">diybio+un...@googlegroups.com</a>.<br></blockquote></div></div><div dir="ltr"><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%40googlegroups.com?utm_medium=email&utm_source=footer" rel="nofollow" target="_blank">https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%40googlegroups.com</a>.<br> </blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature">-Hans T. Wilms<br>Cell: <a href="tel:(404)%20322-5824" value="+14043225824" rel="nofollow" target="_blank">404-322-5824</a></div></div> </blockquote></div> <p></p> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to <a href="mailto:diybio@googlegroups.com" target="_blank">diybio@googlegroups.com</a>. To unsubscribe from this group, send email to <a href="mailto:diybio%2Bunsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" target="_blank">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" target="_blank">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/50bd46c0-4428-455a-9176-afdfeb4214b8n%40googlegroups.com?utm_medium=email&utm_source=footer" target="_blank">https://groups.google.com/d/msgid/diybio/50bd46c0-4428-455a-9176-afdfeb4214b8n%40googlegroups.com</a>.<br> </blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature">-Hans T. Wilms<br>Cell: 404-322-5824</div></div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CAFpMOiTF8UBah8yPyWhq9ci43-KDg1N3s%3Dfi2k7mGmBiPwAJBQ%40mail.gmail.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/CAFpMOiTF8UBah8yPyWhq9ci43-KDg1N3s%3Dfi2k7mGmBiPwAJBQ%40mail.gmail.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-13222143150351559862024-02-29T06:41:00.001-08:002024-02-29T06:41:55.521-08:00[DIYbio] Therapeutic plasma replacement, infusion and exchange<div class="WordSection1"> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif">Members,<o:p></o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif"><o:p> </o:p></span></p> <p class="MsoNormal">My name's Johnny Adams. <o:p></o:p></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif"><o:p> </o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif">I recently moved to Florida to be active in two initiatives that I believe have the greatest potential for biological age management and reversal:<o:p></o:p></span></p> <ul style="margin-top:0in" type="disc"> <li class="MsoListParagraph" style="margin-left:0in;mso-list:l0 level1 lfo1"><span style="font-family:"Arial",sans-serif">Yamanaka/OSK gene therapy to reset the epigenome. A therapy is a few years away.<o:p></o:p></span></li><li class="MsoListParagraph" style="margin-left:0in;mso-list:l0 level1 lfo1"><span style="font-family:"Arial",sans-serif">A plasma project involving therapeutic plasma replacement, infusion and exchange<o:p></o:p></span></li></ul> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif"><o:p> </o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif">It will initially be available in Florida, then in other parts of the country.<o:p></o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif"><o:p> </o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif">More information:<o:p></o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif"><a href="https://www.aginginterventionfoundation.org/JohnnyAdamsPlasmaProjectSummary.pdf">https://www.aginginterventionfoundation.org/JohnnyAdamsPlasmaProjectSummary.pdf</a><o:p></o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif"><o:p> </o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif">Please contact me if you have any interest.<o:p></o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif"><o:p> </o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif">Johnny<o:p></o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif">1-949-922-9786 cell<o:p></o:p></span></p> <p class="MsoNormal"><span style="font-family:"Arial",sans-serif"><a href="mailto:JAdams@AgingInterventionFoundation.org">JAdams@AgingInterventionFoundation.org</a><o:p></o:p></span></p> <p class="MsoNormal"><span style="font-family:"Times New Roman",serif"><o:p> </o:p></span></p> </div> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-74007337587072795302024-02-29T01:52:00.000-08:002024-02-29T01:53:09.056-08:00Re: [DIYbio] Re: DIYbio - Week 3 of Jan 2024 notes poked up to this thingHans, there are a few out there. r/DIYbio (stay away from r/biohackers, it's a bit crazy) the fb group biohacking & genetic engineering, discord SciHouse (which hosted al #DIYbio on twitter. <br /><br /><div><br /></div><div class="gmail_quote"><div dir="auto" class="gmail_attr">On Thursday, February 29, 2024 at 3:38:23 AM UTC+11 Hans Wilms wrote:<br/></div><blockquote class="gmail_quote" style="margin: 0 0 0 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;"><div dir="ltr"><div dir="ltr">Does anyone know where these DIY groups are in the Facebook, Reddit, Discords space? I feel like DIYbio has mostly died off compared to what it was in 2010s, but I'm hoping I'm just out of touch.</div><br><img width="0" height="0" alt="" style="display:flex" src="https://ci4.googleusercontent.com/proxy/_OmQsXDCqRQYmjEDZalHp9aMo_hkNqov_N8B-IhFpcRfkUrlegqCryfMtNwLTUMC223JvBilfOswH2U6pDtd47oimy6mgSs-5J9MgNiuvcBzEgoG9InrOyvRY7ky84e6QZVeNWPZ7KID=s0-d-e1-ft#https://mailtrack.io/trace/mail/c91ab39e6bf9a4522ed3026d6d156f7dd03cb36d.png?u=2280817"><div class="gmail_quote"></div></div><div dir="ltr"><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Feb 28, 2024 at 11:01 AM Dan Kolis <<a href data-email-masked rel="nofollow">dank...@gmail.com</a>> wrote:<br></div></div></div><div dir="ltr"><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><br><br><div><blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div><blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><br>The VC world is plenty happy to convert nutrients to H20 + Urea on a huge scale. I mean, 'hunt the unicorn', then wait for layoffs due to nothing usable seems like a common plan.</blockquote></div></blockquote><div><br> Again in the investment lingo; it is just 'widows and orphans' or do institutions fund endless go nowhere biotech, or what ? I don't know, I have tried to find out, and I am a little interested.<br><br>Like here in Toronto, the teachers Union has thrown in on Quantum computers FOR LIFE SCI, and the Fed and province peeled off millions for little analog computers that do exactly nothing, all blessed with urges based on Stat trek. I mean, That twisty thing on the screen, is that REAL DNA ?". How hard could this be ? Only took 45 seconds for Warf and some hottie to make a cure via an air blown med. Works first time perfectly, before the next commercial !<br><br>Ok. DIY or institutional, non-profit or whatever. Doing what took mother nature 100K years to do before a patent runs out, is not too easy.<br><br>Scale up a hobby, scale down a unicorn. Not so easy. When one atom in a molecule changes hugely what happens, its pretty tough stuff to tame.<br><br>regs,</div><div>Daniel B. Kolis</div><div><br></div><div><br></div><div><br><br><br></div></div> <p></p></blockquote></div></div><div dir="ltr"><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to <a href data-email-masked rel="nofollow">diy...@googlegroups.com</a>. To unsubscribe from this group, send email to <a href data-email-masked rel="nofollow">diybio+un...@googlegroups.com</a>. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://groups.google.com/d/forum/diybio?hl%3Den&source=gmail&ust=1709285244219000&usg=AOvVaw089vMtcpFcq6GS2iB8x-sn">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=http://www.diybio.org&source=gmail&ust=1709285244219000&usg=AOvVaw0mdnoeF7Bm8HfdSVNOqa52">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href data-email-masked rel="nofollow">diybio+un...@googlegroups.com</a>.<br></blockquote></div></div><div dir="ltr"><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%40googlegroups.com?utm_medium=email&utm_source=footer" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%2540googlegroups.com?utm_medium%3Demail%26utm_source%3Dfooter&source=gmail&ust=1709285244219000&usg=AOvVaw2rK6ABKnzxDume8KqLS_U1">https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%40googlegroups.com</a>.<br> </blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature">-Hans T. Wilms<br>Cell: <a href="tel:(404)%20322-5824" value="+14043225824" target="_blank" rel="nofollow">404-322-5824</a></div></div> </blockquote></div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/50bd46c0-4428-455a-9176-afdfeb4214b8n%40googlegroups.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/50bd46c0-4428-455a-9176-afdfeb4214b8n%40googlegroups.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-62952016006052828552024-02-28T08:38:00.001-08:002024-02-28T08:38:28.339-08:00Re: [DIYbio] Re: DIYbio - Week 3 of Jan 2024 notes poked up to this thing<div dir="ltr"><div dir="ltr">Does anyone know where these DIY groups are in the Facebook, Reddit, Discords space? I feel like DIYbio has mostly died off compared to what it was in 2010s, but I'm hoping I'm just out of touch.</div><br><img width="0" height="0" class="mailtrack-img" alt="" style="display:flex" src="https://mailtrack.io/trace/mail/c91ab39e6bf9a4522ed3026d6d156f7dd03cb36d.png?u=2280817"><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Feb 28, 2024 at 11:01 AM Dan Kolis <<a href="mailto:dankolis@gmail.com">dankolis@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><br><br><div><blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div><blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><br>The VC world is plenty happy to convert nutrients to H20 + Urea on a huge scale. I mean, 'hunt the unicorn', then wait for layoffs due to nothing usable seems like a common plan.</blockquote></div></blockquote><div><br> Again in the investment lingo; it is just 'widows and orphans' or do institutions fund endless go nowhere biotech, or what ? I don't know, I have tried to find out, and I am a little interested.<br><br>Like here in Toronto, the teachers Union has thrown in on Quantum computers FOR LIFE SCI, and the Fed and province peeled off millions for little analog computers that do exactly nothing, all blessed with urges based on Stat trek. I mean, That twisty thing on the screen, is that REAL DNA ?". How hard could this be ? Only took 45 seconds for Warf and some hottie to make a cure via an air blown med. Works first time perfectly, before the next commercial !<br><br>Ok. DIY or institutional, non-profit or whatever. Doing what took mother nature 100K years to do before a patent runs out, is not too easy.<br><br>Scale up a hobby, scale down a unicorn. Not so easy. When one atom in a molecule changes hugely what happens, its pretty tough stuff to tame.<br><br>regs,</div><div>Daniel B. Kolis</div><div><br></div><div><br></div><div><br><br><br></div></div> <p></p> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to <a href="mailto:diybio@googlegroups.com" target="_blank">diybio@googlegroups.com</a>. To unsubscribe from this group, send email to <a href="mailto:diybio%2Bunsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" target="_blank">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" target="_blank">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%40googlegroups.com?utm_medium=email&utm_source=footer" target="_blank">https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%40googlegroups.com</a>.<br> </blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature">-Hans T. Wilms<br>Cell: 404-322-5824</div></div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CAFpMOiSjPKP6kZJ6JRJRjxq2pHHQff6syBs7p7yOyDnHiSDstQ%40mail.gmail.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/CAFpMOiSjPKP6kZJ6JRJRjxq2pHHQff6syBs7p7yOyDnHiSDstQ%40mail.gmail.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-62792404367915533082024-02-28T08:01:00.000-08:002024-02-28T08:02:02.737-08:00[DIYbio] Re: DIYbio - Week 3 of Jan 2024 notes poked up to this thing<br /><br /><div><blockquote style="margin: 0px 0px 0px 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;"><div><blockquote style="margin: 0px 0px 0px 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;"><br />The VC world is plenty happy to convert nutrients to H20 + Urea on a huge scale. I mean, 'hunt the unicorn', then wait for layoffs due to nothing usable seems like a common plan.</blockquote></div></blockquote><div><br /> Again in the investment lingo; it is just 'widows and orphans' or do institutions fund endless go nowhere biotech, or what ? I don't know, I have tried to find out, and I am a little interested.<br /><br />Like here in Toronto, the teachers Union has thrown in on Quantum computers FOR LIFE SCI, and the Fed and province peeled off millions for little analog computers that do exactly nothing, all blessed with urges based on Stat trek. I mean, That twisty thing on the screen, is that REAL DNA ?". How hard could this be ? Only took 45 seconds for Warf and some hottie to make a cure via an air blown med. Works first time perfectly, before the next commercial !<br /><br />Ok. DIY or institutional, non-profit or whatever. Doing what took mother nature 100K years to do before a patent runs out, is not too easy.<br /><br />Scale up a hobby, scale down a unicorn. Not so easy. When one atom in a molecule changes hugely what happens, its pretty tough stuff to tame.<br /><br />regs,</div><div>Daniel B. Kolis</div><div><br /></div><div><br /></div><div><br /><br /><br /></div></div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%40googlegroups.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/5254e19d-7967-4e60-954c-40a24e336d03n%40googlegroups.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-2695208207168118392024-02-28T07:48:00.000-08:002024-02-28T07:49:07.480-08:00[DIYbio] A Numerical method mini project in APLthe greasy annoyances of R and SPSS and other kiddie class numerical methods applications has annoyed me enough to do a specific thing.<div><br /></div><div>A coworker has a medium sized life science problem and I think I will throw in on it shortly.<br /><br />I can easily visualize an <b>APL tool for calculus of finite differences</b>, in this case all Abelian integers to enum options of lists of things, that do things....<br /><br />You know, its sure fun to visualize months if tinkering, esp if subsidized, but an afternoon of APL should do it...<br /><br />If anybody 'out there' is interested let me know. I would have to anonymize the results. That's fine.<br /><br />Calculus of finite differences and propagation of constraints are vastly underutilized for whatever reasons... <br /><br />Fun to scrape those <b><font color="#ff0000">Peach tree dishes</font></b> and makes spreadsheets: Sure. Something thinking hard about the output, is a gooder idea too....<br /><br />I might flop out and do nothing, it depends on various variable variables in the people-verse. But if I do does this interest you ?</div><div><br /></div><div>Regs,</div><div>Daniel B. Kolis</div><div><br /></div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/5ae82ea5-4a3c-433a-9af8-9a9d4d4d83dcn%40googlegroups.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/5ae82ea5-4a3c-433a-9af8-9a9d4d4d83dcn%40googlegroups.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-6151697397863122052024-02-27T10:41:00.001-08:002024-02-27T10:41:50.086-08:00[DIYbio] Re: DIYbio - Week 3 of Jan 2024 notes poked up to this thingHi all, i haven't been in this thread for a while, so apologies for the lack of contributions. In response to Dan, I think new platforms are being utilized for DIYbio-related discussions. Facebook, Reddit, Discords, I imagine there are more groups. I guess that's "decentralized" for you! Still, it's awesome to come back and see this Google group still ticking along and it was great to read all your thoughts.<div><br /></div><div>I know from my perspective here in Melbourne Aus, there is a huge appetite for DIYbio in the wake of COVID due to a reduction in the number of hands-on training opportunities for students during our lockdowns. </div><div><br /></div><div>Love the previously mentioned, DITbio, people rarely showed up to just be on their own at our lab.</div><div><br /></div><div>For context of what i'm going to talk about, i was inspired to help start BioQ here in Aus in 2015 (www.bioq.org.au, very much inspired by BioCurious) under a charity structure. From that lab, we saw various needs in our ecosystem and started a science equipment recycling program in 2018 (another charity), and then a biotech CoWorking space called CoLabs Aus in 2020 (finally learned and started a company/social enterprise). </div><div><br /></div><div>BioQ was difficult to run but the community was/is amazing. I didn't have any experience helping run a business, managing revenue, volunteers, projects, and grants, all while living on minimum wage, but i think that was/is par for the course in this space. In the end, i got enough exp doing that to help start CoLabs and learned A LOT about various fields.</div><div><br /></div><div>There is absolutely a challenge with where VCs look at something that is coming out of shared lab spaces like a DIYbio/Community Lab and ask if the IP has been protected, does it have market fit, so on. I've been wondering about this for a while, and i'm exploring this with some IP lawyer friends. </div><div><i><br /></i></div><div><i>Is there a framework/commons that could be the best of both worlds? Support both open access and commercialization at the same time taking into account the needs of those involved?</i> </div><div><br /></div><div>Generally, VCs are happy enough if they see everyone in the space has signed NDAs and consideration has been given to controlled access.</div><div><br /></div><div><br /></div><div><br /><br /></div><div class="gmail_quote"><div dir="auto" class="gmail_attr">On Tuesday, January 23, 2024 at 6:26:51 AM UTC+11 dank...@gmail.com wrote:<br/></div><blockquote class="gmail_quote" style="margin: 0 0 0 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;"><b>Noted :<br>" Reading two books recently: "Open-source lab" by Joshua Pearce and <br></b><b> "Building open source hardware" by Alicia Gibb"</b><b></b><div><b><br></b></div><div>I read the entries to "Dead or what" pretty carefully and didn't really light up with any specific conjectures except maybe to read / get / etc the books above...<br><br><b><font color="#ff0000">Maybe there are some good animals extinct just the right amount and aren't taken yet by Harvard ?<br></font></b><br>You would think pollution abatement might work to getting investments ? Chem companies; like <u>BASF</u>, etc. <br><br>Of course, most Bio projects fail horribly. That's a pretty basic fact, there...<br><br><b>Regs to All,</b></div><div>Dan</div><div><br></div><div><br></div></blockquote></div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/e931a3da-23e3-419f-bdfe-b31a3bf41d27n%40googlegroups.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/e931a3da-23e3-419f-bdfe-b31a3bf41d27n%40googlegroups.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-36252828512434727462024-01-22T08:02:00.001-08:002024-01-22T08:02:41.644-08:00Re: [DIYbio] Is this mini initiative dead ?On 1/22/24 04:39, Aaron Berger wrote:
<br>> The solution I envision is that when one seeks funding or support for a DIY project she should sell the DIYBIO ethos itself. This
<br>> endeavor can lead to a more considered perspective on one's own work (and then someone might try to monetize your idea).
<br>
<br>I think Aaron is onto a big thing here, and I have "a little" to add. Selling is very necessary for any product -- it is wishful
<br>thinking if you skip it. So then once you acknowledge a need for salesmanship/saleswomanship, comes the want for more team
<br>members (to find more salespeople and tech support and accounting support also).
<br>
<br>But is the VC club the only source of investment money and the only terms one can expect? In the low risk, low tech world of real
<br>estate, there are plenty of mechanisms for getting money for projects and they don't even always sell ownership in a startup,
<br>sometimes just loan interest. For example, a shopping center development company is all about construction management, and they
<br>ask around for projects, and some of the projects are parcels of land held for 5 years or so by an investment group, a partnership
<br>usually, so this case uses ownership to extract a return on capital used to purchase land and once the development company
<br>completes the project it is sold retail and the land owners, (more like wholesalers), get paid and get their return. They only
<br>get paid once the retail sale of a shopping center is ripe -- with shoppers ready to shop and traffic passing by the location up
<br>to levels that support enough shoppers to pay the retail rents.
<br>
<br>So, the real estate analogy to a bio startup... Where's that? It will take a little selling to put it in the mind's eye of an
<br>investor, and you need to be an investor yourself. Think of what parts of your plan are very low risk and sell those. If there
<br>are no low risk parts, your plan is toast, DOA, silly. The real estate analogs are the office you work in, the lab you build and
<br>test in, and your track record of staying alive in business and paying your taxes.
<br>
<br>The VC know that some parts of many plans are low risk after a substantial wait, and they decide to risk some accordingly, with
<br>the knowledge that liquidating will recover some of the original investment and one success in ten will pay for all the rest. The
<br>real estate type of investor won't suffer the VC level of risk even -- they demand that company liquidation returns more than the
<br>investment during the life of the demographics waiting period before the building developer is involved and during project
<br>development.
<br>
<br>The part where you be the investor is analogous to the demographics waiting period before project development. You build a team
<br>working on something very low risk and get them ready to keep the risk low during the ultimate project you envision. This may
<br>seem hard to face -- that you cannot leap into what inspires you, but first have to build a functioning team, and your works is
<br>something less exciting for a while and you have to *SELL* this less exciting concept. If that's unimaginable to you, the VC are
<br>all that's left for your funding.
<br>
<br>How would we sell the idea of a low risk, "training wheels" project, and to whom? Well, for example, I did a project with less
<br>than necessary capital to arrive at a half-baked result, an electroporator with no plastic case, not ready for prime time since no
<br>safety testing done yet, (since no protective case with safety interlocks against high voltage dangers), so incomplete and not a
<br>speed up tool. The capital I could have had easily is the getting ready kind -- just finding some grunt work to pay the bills and
<br>getting a survivor track record created. Then I could have shown my product ideas to real estate types of investors and they
<br>would have few objections.
<br>
<br>It's not easy to find grunt work to do to prove you can be in business, but it's not easy to make a home lab and many of us have
<br>done that and can imagine a little more prep work before getting into the exciting phases of creation.
<br>
<br>Speed is a very important thing. I was just about ready to get some techie grunt work, but lost my lab. My lab was in a house in
<br>Austin TX as things started to get very expensive there -- like NYC or SF or LA, and the house needed to be sold. That reset the
<br>timeline. Another thing is I got older -- some phases come ready or not.
<br>
<br>So now I have a building block controller that can be the button pushing, user programmable in python, small readout displaying,
<br>sequence generating, measuring, power converting guts to most any lab instrument of solar charger/converter/battery-manager.
<br>Know anyone needing these kinds of guts for a project that are OSHW and some help using them? I'm for hire.
<br>
<br>
<br>--
<br>John Griessen in Albuquerque NM building lab gear for biologists
<br>
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<br>To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/ef327279-0fa2-40db-81dd-3fdb41ccb915%40industromatic.com">https://groups.google.com/d/msgid/diybio/ef327279-0fa2-40db-81dd-3fdb41ccb915%40industromatic.com</a>.Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-70339905135298517522024-01-17T09:17:00.001-08:002024-01-17T09:17:31.963-08:00Re: [DIYbio] Is this mini initiative dead ?On 1/17/24 10:06, Nathan McCorkle wrote:
<br>> Working now in a corporate engineering day-job, it's more and more apparent that "do it together" gets things done faster and
<br>> possibly with less stress, although the type of stress is also a different form.
<br>
<br>DITBIO! OK, and for most real life enthusiasts, it takes capital too...
<br>
<br>So, the new DITBIO group proposed by Nathan needs bookkeeping, corporation law, entrepreneurship, and maybe even stock trading
<br>classes to go along with it. In the US S-corps that pass through gains or losses to individual tax returns are the way to make a
<br>far flung group work. Internationally it could be possible, but I don't know how feasible to have a far flung across boundaries
<br>group that shares in profits and funding by debt, or direct contribution.
<br>
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<br>To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/1ec7c0dc-624f-40c8-b749-39846cfdcc6d%40industromatic.com">https://groups.google.com/d/msgid/diybio/1ec7c0dc-624f-40c8-b749-39846cfdcc6d%40industromatic.com</a>.Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-14476049389541692982024-01-17T09:06:00.001-08:002024-01-17T09:06:50.615-08:00Re: [DIYbio] Is this mini initiative dead ?<div dir="ltr">I like to think, we're all busy in the lab!<div><br></div><div>My goals really haven't changed from the bulk of my past postings. It's a lot of work reaching these goals. Moreso when you're using the "DIY" approach. Working now in a corporate engineering day-job, it's more and more apparent that "do it together" gets things done faster and possibly with less stress, although the type of stress is also a different form.</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Jan 17, 2024 at 8:22 AM Dakota Hamill <<a href="mailto:dkotes@gmail.com">dkotes@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="ltr">Hello Sad Dan.<div><br></div><div>Group definitely used to be more active, but many of us still lurk. I think many people "grew up" and finished school, started their careers, had to get real jobs, life, responsibilities, all that jazz. Not all hobbies pay the bills. Some went on to start companies, very large push to move everything to closed source once you get funding. <div><br></div><div>I've been reading 2 books recently called "Open-source lab" by Joshua Pearce and "Building open source hardware" by Alicia Gibb to understand more of the companies that have used that business model successfully. </div></div><div><br></div><div>Science is awesome, but it doesn't always pay the bills, and that sucks. </div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Jan 17, 2024 at 9:40 AM Dan Kolis <<a href="mailto:dankolis@gmail.com" target="_blank">dankolis@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">I sadly note no material here, generally.<div><br></div><div>Sad to think DIY biotech just went nowhere, or what ?<br><br>hmmm,</div><div><br></div><div>Sad Dan in Toronto</div><div><br></div><div><br></div> <p></p> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" target="_blank">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" target="_blank">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CAGdeWmTb4rhFy5hwQTHn-TRiK%2BnAZHJpATjaiGiUAGpXxdRCdA%40mail.gmail.com?utm_medium=email&utm_source=footer" target="_blank">https://groups.google.com/d/msgid/diybio/CAGdeWmTb4rhFy5hwQTHn-TRiK%2BnAZHJpATjaiGiUAGpXxdRCdA%40mail.gmail.com</a>.<br> </blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature">-Nathan</div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CA%2B82U9KMj6KZ0aO5Kczksn2qsFXjCyA0Bvk_QP1nE_9r2DSwxA%40mail.gmail.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/CA%2B82U9KMj6KZ0aO5Kczksn2qsFXjCyA0Bvk_QP1nE_9r2DSwxA%40mail.gmail.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-55121850576076125152024-01-17T09:05:00.000-08:002024-01-17T09:06:29.477-08:00Re: [DIYbio] Is this mini initiative dead ?On 1/17/24 09:22, Dakota Hamill wrote:
<br>> very large push to move everything to closed source once you get funding.
<br>
<br>+1
<br>
<br>> I've been reading 2 books recently called "Open-source lab" by Joshua Pearce and "Building open source hardware" by Alicia Gibb to
<br>> understand more of the companies that have used that business model successfully.
<br>
<br>Thanks for those.
<br>
<br>I lost some courage for OSHW after making a nice functioning electroporator circuit, (culture shock), when I got little testing
<br>help or even suggestions for what would make it the best speed tool. It's a high voltage tool, so safety testing is needed and
<br>expensive, and then there's plastic molding for even just bare usability -- brick boxes don't cut it when you need safety
<br>interlock doors. Lacking a finished usable UI and enclosure with buttons and readouts stops most all from testing it seems. So
<br>then you're in non-open trade secret mode to survive instead of go bankrupt.
<br>
<br>So now I'm lo0king into "low pressure slow", but full auto plastic molding. That kind of thing, (with 3DP tooling), will enable
<br>small product testing runs of 100s cheaply.
<br>
<br>--
<br>John Griessen
<br>1 bankruptcy, never again...
<br>
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<br>To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/6bcf92e5-e1f1-4e5c-8d38-30d730528e65%40industromatic.com">https://groups.google.com/d/msgid/diybio/6bcf92e5-e1f1-4e5c-8d38-30d730528e65%40industromatic.com</a>.Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-5802662306768112412023-09-06T15:08:00.000-07:002023-09-06T15:09:03.218-07:00Re: [DIYbio] Software for Knowledgebase<div style="font-family: Arial, sans-serif; font-size: 14px;">Dakota,</div><div style="font-family: Arial, sans-serif; font-size: 14px;"><br></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://www.zettlr.com/">https://www.zettlr.com/</a>. Is a great one (it is not just for publications)</span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://logseq.com/">https://logseq.com/</a><br></span></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><br></span></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span>Local Wiki (with some options to share and hosting):</span></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://tiddlywiki.com/">https://tiddlywiki.com/</a></span><br></span></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><br></span></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span>For the more text/code editor savvy:</span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://orgmode.org/">https://orgmode.org/</a></span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://github.com/Furkanzmc/zettelkasten.nvim">https://github.com/Furkanzmc/zettelkasten.nvim</a></span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://www.dendron.so/">https://www.dendron.so/</a></span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span>Commercial products with good reputation and free plans:</span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://www.notion.so/">https://www.notion.so/</a></span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://obsidian.md/">https://obsidian.md/</a></span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span>Not tested but in a similar vein:</span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><span><a target="_blank" rel="noreferrer nofollow noopener" href="https://appflowy.io/">https://appflowy.io/</a></span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"><br></div><div style="font-family: Arial, sans-serif; font-size: 14px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">There are obviously a lot more, but that should get you started.<br></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span><br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><span>+<br></span></div><div style="font-family: Arial, sans-serif; font-size: 14px;"><br></div> <div class="protonmail_signature_block protonmail_signature_block-empty" style="font-family: Arial, sans-serif; font-size: 14px;"> <div class="protonmail_signature_block-user protonmail_signature_block-empty"></div> <div class="protonmail_signature_block-proton protonmail_signature_block-empty"> </div> </div> <div style="font-family: Arial, sans-serif; font-size: 14px;"><br></div><div class="protonmail_quote"> ------- Original Message -------<br> On Wednesday, September 6th, 2023 at 4:56 PM, Dakota Hamill <dkotes@gmail.com> wrote:<br><br> <blockquote class="protonmail_quote" type="cite"> Hello. I am effectively coding-illiterate but I'd like to start assembling a knowledge base of science protocols, methods, techniques etc.<div><br></div><div>I know there are Wiki's, but I've not found one to install onto a private domain that doesn't require a bit of coding expertise.</div><div><br></div><div>I don't like the feel of making it in Google Docs as I don't want one giant word document.</div><div><br></div><div>GitHub looks clean but with zero coding experience, I've never made much use of it.</div><div><br></div><div>I recently stumbled upon GitBook and Docusaurus and they both look interesting in making clean and easy looking knowledge databases.</div><div><br></div><div>How different are they from a traditional wiki? </div><div><br></div><div>Can GitBook be hosted privately, ever, or will all the data always be on "GitHub's" servers?</div><div><br></div><div>Thank you, Dakota</div><div><br></div><div><br></div> <p></p> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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Allow bread to sit outside and become infected with mold (type of fungus)</div> <div>2. Take 1 sample of this mold (some of them can be very nasty to human health if mold spores i.e. dust, is inhaled, especially by immuno compromised individuals)</div> <div>3. treat this mold sample (dust) for either different amounts of times (30 sec, 1 min, 5min, 10min, 30min) and / or different concentrations (undiluted commercial bleach, and 5 serial dilutions with dilution factor of 2 each time , so 2, 4, 8, 16, 32 would be the dilution factor)</div> <div>4. Take these treated mold powder samples, and put them on a new slice of bread, and leave out</div> <div>5. As a control experiment, leave out an uninoculated slice of bread</div> <div>6. Expected results: No growth or slower (RE)growth of mold when it has been treated for longer and / or with more concentrated bleach</div> <div><br> </div> <div>This suggested protocol obviates the need for LB medium or plates etc etc<br> And it is perhaps way more intuitive to understand, especially for really young participants or those with no scientific bent of mind, yet.</div> <div><br> </div> <div>Because things are less controlled i.e leaving bread outside is not the most controlled experiment, explaining variations in results between replicated experiments will take some explaining.</div> <div>Apart from variation in results, you may obtain unexpected results, based on random / (un)lucky events, e.g. the uninoculated bread slice has way more mold, but a different color mold</div> <div>So on and so forth.</div> <div><br> </div> <div>But this protocol should easily fit within your $10 budget for a cohort of 5-10 learners. You would only need to but</div> <div>commercial bleach - $5</div> <div>bread - $5</div> <div>tubes / containers to perform the serial dilution in - borrow from some lab?</div> <div>pipette to perform the serial dilution with - borrow from some lab?</div> <div>timer to count minutes of bleach treatment (use watch / smartwatch / computer clock)</div> <div><br> </div> <div>Cheers!</div> <div>TdM</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 20, 2023 at 9:46 PM Markos <<a href="" data-email-masked="" rel="nofollow" moz-do-not-send="true">mar...@c2o.pro.br</a>> wrote:<br> </div> </div> <div class="gmail_quote"> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Nathan,</p> <p>You suggest this type of microscope?</p> <p><a href="https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/&source=gmail&ust=1692771562088000&usg=AOvVaw0upeTZK9zCNQlqLheRI-E-" moz-do-not-send="true" class="moz-txt-link-freetext">https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/</a></p> <p><a href="https://makezine.com/projects/laser-projection-microscope/" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://makezine.com/projects/laser-projection-microscope/&source=gmail&ust=1692771562088000&usg=AOvVaw01zK9Fiuy1XLZcwFYUeogI" moz-do-not-send="true" class="moz-txt-link-freetext">https://makezine.com/projects/laser-projection-microscope/</a></p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 20/08/2023 19:34, Nathan McCorkle escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">Will you be able to have a microscope available, or maybe the projection microscope that shines a laser through a hanging droplet? I wonder if you could find a yeast cell, then add some bleach and watch it dissolve. </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Wed, Aug 16, 2023 at 4:08 PM Markos <<a href="" data-email-masked="" rel="nofollow" moz-do-not-send="true">mar...@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Tom,</p> <p>4 to 6 participants at a time because space is small</p> <p>US$10,00 for each group of 4 to 6 participants</p> <p>The idea is to use scrap and buy only what is strictly necessary, such as agar (for edible use).<br> </p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 16/08/2023 12:17, Tom De Medts escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details: <div>1. No. of participants (maximum expected), and</div> <div>2. Your maximum budget.</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a href="" data-email-masked="" rel="nofollow" moz-do-not-send="true">mar...@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer nofollow" target="_blank" data-saferedirecturl="https://www.google.com/url?hl=en&q=http://www.c2o.pro.br&source=gmail&ust=1692771562088000&usg=AOvVaw1nJ3K_35HBrZerwT2bP6EF" moz-do-not-send="true">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" moz-do-not-send="true" class="moz-txt-link-freetext">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a class="moz-txt-link-abbreviated" href="http://www.diybio.org">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" moz-do-not-send="true" class="moz-txt-link-freetext">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/0d8031c3-114d-403b-bd4c-3daa8c3fad47n%40googlegroups.com?utm_medium=email&utm_source=footer" moz-do-not-send="true">https://groups.google.com/d/msgid/diybio/0d8031c3-114d-403b-bd4c-3daa8c3fad47n%40googlegroups.com</a>.<br> </blockquote> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-49395233285245643512023-08-22T12:33:00.001-07:002023-08-22T12:33:54.754-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<p>Hi Tom,<br> </p> <p>Very cool! :-)</p> <p>Thanks,</p> <p>Markos<br> </p> <div class="moz-cite-prefix">Em 21/08/2023 08:08, Tom De Medts escreveu:<br> </div> <blockquote type="cite" cite="mid:CANpz=fEJH2-m0vWt4zrY0v8m_8pWSMSJ68QeBNL1khYHEmbnzQ@mail.gmail.com"> <meta http-equiv="content-type" content="text/html; charset=UTF-8"> <div dir="ltr">Here is my suggested protocol #1: <div><br> </div> <div>1. Allow bread to sit outside and become infected with mold (type of fungus)</div> <div>2. Take 1 sample of this mold (some of them can be very nasty to human health if mold spores i.e. dust, is inhaled, especially by immuno compromised individuals)</div> <div>3. treat this mold sample (dust) for either different amounts of times (30 sec, 1 min, 5min, 10min, 30min) and / or different concentrations (undiluted commercial bleach, and 5 serial dilutions with dilution factor of 2 each time , so 2, 4, 8, 16, 32 would be the dilution factor)</div> <div>4. Take these treated mold powder samples, and put them on a new slice of bread, and leave out</div> <div>5. As a control experiment, leave out an uninoculated slice of bread</div> <div>6. Expected results: No growth or slower (RE)growth of mold when it has been treated for longer and / or with more concentrated bleach</div> <div><br> </div> <div>This suggested protocol obviates the need for LB medium or plates etc etc<br> And it is perhaps way more intuitive to understand, especially for really young participants or those with no scientific bent of mind, yet.</div> <div><br> </div> <div>Because things are less controlled i.e leaving bread outside is not the most controlled experiment, explaining variations in results between replicated experiments will take some explaining.</div> <div>Apart from variation in results, you may obtain unexpected results, based on random / (un)lucky events, e.g. the uninoculated bread slice has way more mold, but a different color mold</div> <div>So on and so forth.</div> <div><br> </div> <div>But this protocol should easily fit within your $10 budget for a cohort of 5-10 learners. You would only need to but</div> <div>commercial bleach - $5</div> <div>bread - $5</div> <div>tubes / containers to perform the serial dilution in - borrow from some lab?</div> <div>pipette to perform the serial dilution with - borrow from some lab?</div> <div>timer to count minutes of bleach treatment (use watch / smartwatch / computer clock)</div> <div><br> </div> <div>Cheers!</div> <div>TdM</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 20, 2023 at 9:46 PM Markos <<a href="mailto:markos@c2o.pro.br" moz-do-not-send="true" class="moz-txt-link-freetext">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Nathan,</p> <p>You suggest this type of microscope?</p> <p><a href="https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/" target="_blank" moz-do-not-send="true" class="moz-txt-link-freetext">https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/</a></p> <p><a href="https://makezine.com/projects/laser-projection-microscope/" target="_blank" moz-do-not-send="true" class="moz-txt-link-freetext">https://makezine.com/projects/laser-projection-microscope/</a></p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 20/08/2023 19:34, Nathan McCorkle escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">Will you be able to have a microscope available, or maybe the projection microscope that shines a laser through a hanging droplet? I wonder if you could find a yeast cell, then add some bleach and watch it dissolve. </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Wed, Aug 16, 2023 at 4:08 PM Markos <<a href="mailto:markos@c2o.pro.br" target="_blank" moz-do-not-send="true" class="moz-txt-link-freetext">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Tom,</p> <p>4 to 6 participants at a time because space is small</p> <p>US$10,00 for each group of 4 to 6 participants</p> <p>The idea is to use scrap and buy only what is strictly necessary, such as agar (for edible use).<br> </p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 16/08/2023 12:17, Tom De Medts escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details: <div>1. No. of participants (maximum expected), and</div> <div>2. Your maximum budget.</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a href="mailto:markos@c2o.pro.br" target="_blank" moz-do-not-send="true" class="moz-txt-link-freetext">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer" target="_blank" moz-do-not-send="true">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" moz-do-not-send="true" class="moz-txt-link-freetext">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a class="moz-txt-link-abbreviated" href="http://www.diybio.org">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" moz-do-not-send="true" class="moz-txt-link-freetext">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CANpz%3DfEJH2-m0vWt4zrY0v8m_8pWSMSJ68QeBNL1khYHEmbnzQ%40mail.gmail.com?utm_medium=email&utm_source=footer" moz-do-not-send="true">https://groups.google.com/d/msgid/diybio/CANpz%3DfEJH2-m0vWt4zrY0v8m_8pWSMSJ68QeBNL1khYHEmbnzQ%40mail.gmail.com</a>.<br> </blockquote> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-49057631769249858242023-08-22T04:52:00.001-07:002023-08-22T04:52:58.426-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<div dir="ltr">For the sake of all those who eat at McDonalds I hope this is not true. <div> But ROFLMAO! Good one, Jonathan Cline. <font size="6">😂😆</font><br></div><div>- TdM</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Tue, Aug 22, 2023 at 2:21 AM Jonathan Cline <<a href="mailto:jncline@gmail.com">jncline@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"><br>Bonus if this includes buying a McDonalds hamburger and placing it out with the bread; then explaining why the McDonalds food never grows any mold at all, even compared to the bleached control.<div><br></div><div class="gmail_quote"><div dir="auto" class="gmail_attr">On Monday, August 21, 2023 at 4:08:30 AM UTC-7 Tom De Medts wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"><div dir="ltr">Here is my suggested protocol #1:<div><br></div><div>1. Allow bread to sit outside and become infected with mold (type of fungus)</div><div>2. Take 1 sample of this mold (some of them can be very nasty to human health if mold spores i.e. dust, is inhaled, especially by immuno compromised individuals)</div><div>3. treat this mold sample (dust) for either different amounts of times (30 sec, 1 min, 5min, 10min, 30min) and / or different concentrations (undiluted commercial bleach, and 5 serial dilutions with dilution factor of 2 each time , so 2, 4, 8, 16, 32 would be the dilution factor)</div><div>4. Take these treated mold powder samples, and put them on a new slice of bread, and leave out</div><div>5. As a control experiment, leave out an uninoculated slice of bread</div><div>6. Expected results: No growth or slower (RE)growth of mold when it has been treated for longer and / or with more concentrated bleach</div><div><br></div><div>This suggested protocol obviates the need for LB medium or plates etc etc<br>And it is perhaps way more intuitive to understand, especially for really young participants or those with no scientific bent of mind, yet.</div><div><br></div><div>Because things are less controlled i.e leaving bread outside is not the most controlled experiment, explaining variations in results between replicated experiments will take some explaining.</div><div>Apart from variation in results, you may obtain unexpected results, based on random / (un)lucky events, e.g. the uninoculated bread slice has way more mold, but a different color mold</div><div>So on and so forth.</div><div><br></div><div>But this protocol should easily fit within your $10 budget for a cohort of 5-10 learners. You would only need to but</div><div>commercial bleach - $5</div><div>bread - $5</div><div>tubes / containers to perform the serial dilution in - borrow from some lab?</div><div>pipette to perform the serial dilution with - borrow from some lab?</div><div>timer to count minutes of bleach treatment (use watch / smartwatch / computer clock)</div><div><br></div><div>Cheers!</div><div>TdM</div></div><br><div class="gmail_quote"></div><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Sun, Aug 20, 2023 at 9:46 PM Markos <<a rel="nofollow">mar...@c2o.pro.br</a>> wrote:<br></div></div><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Nathan,</p> <p>You suggest this type of microscope?</p> <p><a href="https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/" rel="nofollow" target="_blank">https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/</a></p> <p><a href="https://makezine.com/projects/laser-projection-microscope/" rel="nofollow" target="_blank">https://makezine.com/projects/laser-projection-microscope/</a></p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 20/08/2023 19:34, Nathan McCorkle escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">Will you be able to have a microscope available, or maybe the projection microscope that shines a laser through a hanging droplet? I wonder if you could find a yeast cell, then add some bleach and watch it dissolve. </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Wed, Aug 16, 2023 at 4:08 PM Markos <<a rel="nofollow">mar...@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Tom,</p> <p>4 to 6 participants at a time because space is small</p> <p>US$10,00 for each group of 4 to 6 participants</p> <p>The idea is to use scrap and buy only what is strictly necessary, such as agar (for edible use).<br> </p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 16/08/2023 12:17, Tom De Medts escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details: <div>1. No. of participants (maximum expected), and</div> <div>2. Your maximum budget.</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a rel="nofollow">mar...@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer nofollow" target="_blank">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CANpz%3DfFiWUHsOdum0YxtRDHWjH4QNWgSdQ44iktfPzntvobCNQ%40mail.gmail.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/CANpz%3DfFiWUHsOdum0YxtRDHWjH4QNWgSdQ44iktfPzntvobCNQ%40mail.gmail.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-18371962246160057272023-08-21T23:21:00.001-07:002023-08-21T23:21:41.750-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<br />Bonus if this includes buying a McDonalds hamburger and placing it out with the bread; then explaining why the McDonalds food never grows any mold at all, even compared to the bleached control.<div><br /></div><div class="gmail_quote"><div dir="auto" class="gmail_attr">On Monday, August 21, 2023 at 4:08:30 AM UTC-7 Tom De Medts wrote:<br/></div><blockquote class="gmail_quote" style="margin: 0 0 0 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;"><div dir="ltr">Here is my suggested protocol #1:<div><br></div><div>1. Allow bread to sit outside and become infected with mold (type of fungus)</div><div>2. Take 1 sample of this mold (some of them can be very nasty to human health if mold spores i.e. dust, is inhaled, especially by immuno compromised individuals)</div><div>3. treat this mold sample (dust) for either different amounts of times (30 sec, 1 min, 5min, 10min, 30min) and / or different concentrations (undiluted commercial bleach, and 5 serial dilutions with dilution factor of 2 each time , so 2, 4, 8, 16, 32 would be the dilution factor)</div><div>4. Take these treated mold powder samples, and put them on a new slice of bread, and leave out</div><div>5. As a control experiment, leave out an uninoculated slice of bread</div><div>6. Expected results: No growth or slower (RE)growth of mold when it has been treated for longer and / or with more concentrated bleach</div><div><br></div><div>This suggested protocol obviates the need for LB medium or plates etc etc<br>And it is perhaps way more intuitive to understand, especially for really young participants or those with no scientific bent of mind, yet.</div><div><br></div><div>Because things are less controlled i.e leaving bread outside is not the most controlled experiment, explaining variations in results between replicated experiments will take some explaining.</div><div>Apart from variation in results, you may obtain unexpected results, based on random / (un)lucky events, e.g. the uninoculated bread slice has way more mold, but a different color mold</div><div>So on and so forth.</div><div><br></div><div>But this protocol should easily fit within your $10 budget for a cohort of 5-10 learners. You would only need to but</div><div>commercial bleach - $5</div><div>bread - $5</div><div>tubes / containers to perform the serial dilution in - borrow from some lab?</div><div>pipette to perform the serial dilution with - borrow from some lab?</div><div>timer to count minutes of bleach treatment (use watch / smartwatch / computer clock)</div><div><br></div><div>Cheers!</div><div>TdM</div></div><br><div class="gmail_quote"></div><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Sun, Aug 20, 2023 at 9:46 PM Markos <<a href data-email-masked rel="nofollow">mar...@c2o.pro.br</a>> wrote:<br></div></div><div class="gmail_quote"><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Nathan,</p> <p>You suggest this type of microscope?</p> <p><a href="https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/&source=gmail&ust=1692771562088000&usg=AOvVaw0upeTZK9zCNQlqLheRI-E-">https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/</a></p> <p><a href="https://makezine.com/projects/laser-projection-microscope/" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://makezine.com/projects/laser-projection-microscope/&source=gmail&ust=1692771562088000&usg=AOvVaw01zK9Fiuy1XLZcwFYUeogI">https://makezine.com/projects/laser-projection-microscope/</a></p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 20/08/2023 19:34, Nathan McCorkle escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">Will you be able to have a microscope available, or maybe the projection microscope that shines a laser through a hanging droplet? I wonder if you could find a yeast cell, then add some bleach and watch it dissolve. </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Wed, Aug 16, 2023 at 4:08 PM Markos <<a href data-email-masked rel="nofollow">mar...@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Tom,</p> <p>4 to 6 participants at a time because space is small</p> <p>US$10,00 for each group of 4 to 6 participants</p> <p>The idea is to use scrap and buy only what is strictly necessary, such as agar (for edible use).<br> </p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 16/08/2023 12:17, Tom De Medts escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details: <div>1. No. of participants (maximum expected), and</div> <div>2. Your maximum budget.</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a href data-email-masked rel="nofollow">mar...@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer nofollow" target="_blank" data-saferedirecturl="https://www.google.com/url?hl=en&q=http://www.c2o.pro.br&source=gmail&ust=1692771562088000&usg=AOvVaw1nJ3K_35HBrZerwT2bP6EF">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/0d8031c3-114d-403b-bd4c-3daa8c3fad47n%40googlegroups.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/0d8031c3-114d-403b-bd4c-3daa8c3fad47n%40googlegroups.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-80258985149815083662023-08-21T04:08:00.001-07:002023-08-21T04:08:34.853-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<div dir="ltr">Here is my suggested protocol #1:<div><br></div><div>1. Allow bread to sit outside and become infected with mold (type of fungus)</div><div>2. Take 1 sample of this mold (some of them can be very nasty to human health if mold spores i.e. dust, is inhaled, especially by immuno compromised individuals)</div><div>3. treat this mold sample (dust) for either different amounts of times (30 sec, 1 min, 5min, 10min, 30min) and / or different concentrations (undiluted commercial bleach, and 5 serial dilutions with dilution factor of 2 each time , so 2, 4, 8, 16, 32 would be the dilution factor)</div><div>4. Take these treated mold powder samples, and put them on a new slice of bread, and leave out</div><div>5. As a control experiment, leave out an uninoculated slice of bread</div><div>6. Expected results: No growth or slower (RE)growth of mold when it has been treated for longer and / or with more concentrated bleach</div><div><br></div><div>This suggested protocol obviates the need for LB medium or plates etc etc<br>And it is perhaps way more intuitive to understand, especially for really young participants or those with no scientific bent of mind, yet.</div><div><br></div><div>Because things are less controlled i.e leaving bread outside is not the most controlled experiment, explaining variations in results between replicated experiments will take some explaining.</div><div>Apart from variation in results, you may obtain unexpected results, based on random / (un)lucky events, e.g. the uninoculated bread slice has way more mold, but a different color mold</div><div>So on and so forth.</div><div><br></div><div>But this protocol should easily fit within your $10 budget for a cohort of 5-10 learners. You would only need to but</div><div>commercial bleach - $5</div><div>bread - $5</div><div>tubes / containers to perform the serial dilution in - borrow from some lab?</div><div>pipette to perform the serial dilution with - borrow from some lab?</div><div>timer to count minutes of bleach treatment (use watch / smartwatch / computer clock)</div><div><br></div><div>Cheers!</div><div>TdM</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Sun, Aug 20, 2023 at 9:46 PM Markos <<a href="mailto:markos@c2o.pro.br">markos@c2o.pro.br</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Nathan,</p> <p>You suggest this type of microscope?</p> <p><a href="https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/" target="_blank">https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/</a></p> <p><a href="https://makezine.com/projects/laser-projection-microscope/" target="_blank">https://makezine.com/projects/laser-projection-microscope/</a></p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 20/08/2023 19:34, Nathan McCorkle escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">Will you be able to have a microscope available, or maybe the projection microscope that shines a laser through a hanging droplet? I wonder if you could find a yeast cell, then add some bleach and watch it dissolve. </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Wed, Aug 16, 2023 at 4:08 PM Markos <<a href="mailto:markos@c2o.pro.br" target="_blank">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Tom,</p> <p>4 to 6 participants at a time because space is small</p> <p>US$10,00 for each group of 4 to 6 participants</p> <p>The idea is to use scrap and buy only what is strictly necessary, such as agar (for edible use).<br> </p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 16/08/2023 12:17, Tom De Medts escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details: <div>1. No. of participants (maximum expected), and</div> <div>2. Your maximum budget.</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a href="mailto:markos@c2o.pro.br" target="_blank">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer" target="_blank">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CANpz%3DfEJH2-m0vWt4zrY0v8m_8pWSMSJ68QeBNL1khYHEmbnzQ%40mail.gmail.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/CANpz%3DfEJH2-m0vWt4zrY0v8m_8pWSMSJ68QeBNL1khYHEmbnzQ%40mail.gmail.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-61681167087134958012023-08-20T18:46:00.001-07:002023-08-20T18:46:23.100-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<p>Hi Nathan,</p> <p>You suggest this type of microscope?</p> <p><a class="moz-txt-link-freetext" href="https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/">https://mad-science.wonderhowto.com/how-to/build-your-own-projection-microscope-with-syringe-laser-pointer-and-drop-water-0138420/</a></p> <p><a class="moz-txt-link-freetext" href="https://makezine.com/projects/laser-projection-microscope/">https://makezine.com/projects/laser-projection-microscope/</a></p> <p>Thanks,</p> <p>Markos<br> </p> <div class="moz-cite-prefix">Em 20/08/2023 19:34, Nathan McCorkle escreveu:<br> </div> <blockquote type="cite" cite="mid:CA+82U9Jm14YDSrtApKC5cs8HCqpBs27CiZeBEPBPBfRCgyUKyw@mail.gmail.com"> <meta http-equiv="content-type" content="text/html; charset=UTF-8"> <div dir="ltr">Will you be able to have a microscope available, or maybe the projection microscope that shines a laser through a hanging droplet? I wonder if you could find a yeast cell, then add some bleach and watch it dissolve. </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Wed, Aug 16, 2023 at 4:08 PM Markos <<a href="mailto:markos@c2o.pro.br" moz-do-not-send="true" class="moz-txt-link-freetext">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Tom,</p> <p>4 to 6 participants at a time because space is small</p> <p>US$10,00 for each group of 4 to 6 participants</p> <p>The idea is to use scrap and buy only what is strictly necessary, such as agar (for edible use).<br> </p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 16/08/2023 12:17, Tom De Medts escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details: <div>1. No. of participants (maximum expected), and</div> <div>2. Your maximum budget.</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a href="mailto:markos@c2o.pro.br" target="_blank" moz-do-not-send="true" class="moz-txt-link-freetext">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer" target="_blank" moz-do-not-send="true">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" moz-do-not-send="true" class="moz-txt-link-freetext">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a class="moz-txt-link-abbreviated" href="http://www.diybio.org">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" moz-do-not-send="true" class="moz-txt-link-freetext">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CA%2B82U9Jm14YDSrtApKC5cs8HCqpBs27CiZeBEPBPBfRCgyUKyw%40mail.gmail.com?utm_medium=email&utm_source=footer" moz-do-not-send="true">https://groups.google.com/d/msgid/diybio/CA%2B82U9Jm14YDSrtApKC5cs8HCqpBs27CiZeBEPBPBfRCgyUKyw%40mail.gmail.com</a>.<br> </blockquote> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-68577883031202778532023-08-20T15:34:00.001-07:002023-08-20T15:34:53.079-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<div dir="ltr">Will you be able to have a microscope available, or maybe the projection microscope that shines a laser through a hanging droplet? I wonder if you could find a yeast cell, then add some bleach and watch it dissolve. </div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Wed, Aug 16, 2023 at 4:08 PM Markos <<a href="mailto:markos@c2o.pro.br">markos@c2o.pro.br</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"> <div> <p>Hi Tom,</p> <p>4 to 6 participants at a time because space is small</p> <p>US$10,00 for each group of 4 to 6 participants</p> <p>The idea is to use scrap and buy only what is strictly necessary, such as agar (for edible use).<br> </p> <p>Thanks,</p> <p>Markos<br> </p> <div>Em 16/08/2023 12:17, Tom De Medts escreveu:<br> </div> <blockquote type="cite"> <div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details: <div>1. No. of participants (maximum expected), and</div> <div>2. Your maximum budget.</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a href="mailto:markos@c2o.pro.br" target="_blank">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer" target="_blank">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" rel="noreferrer" target="_blank">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" rel="noreferrer" target="_blank">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio%2Bunsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/7f44d614-3d18-85cc-f8dc-c25be3dfe9ae%40c2o.pro.br" rel="noreferrer" target="_blank">https://groups.google.com/d/msgid/diybio/7f44d614-3d18-85cc-f8dc-c25be3dfe9ae%40c2o.pro.br</a>.<br> </blockquote> </div> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" target="_blank">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" target="_blank">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/e3eb19c7-9b6d-b325-5df3-1c242e516cba%40c2o.pro.br?utm_medium=email&utm_source=footer" target="_blank">https://groups.google.com/d/msgid/diybio/e3eb19c7-9b6d-b325-5df3-1c242e516cba%40c2o.pro.br</a>.<br> </blockquote></div><br clear="all"><div><br></div><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature">-Nathan</div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CA%2B82U9Jm14YDSrtApKC5cs8HCqpBs27CiZeBEPBPBfRCgyUKyw%40mail.gmail.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/CA%2B82U9Jm14YDSrtApKC5cs8HCqpBs27CiZeBEPBPBfRCgyUKyw%40mail.gmail.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-73443464818246965752023-08-16T16:08:00.001-07:002023-08-16T16:08:53.694-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<p>Hi Tom,</p> <p>4 to 6 participants at a time because space is small</p> <p>US$10,00 for each group of 4 to 6 participants</p> <p>The idea is to use scrap and buy only what is strictly necessary, such as agar (for edible use).<br> </p> <p>Thanks,</p> <p>Markos<br> </p> <div class="moz-cite-prefix">Em 16/08/2023 12:17, Tom De Medts escreveu:<br> </div> <blockquote type="cite" cite="mid:CANpz=fE7PJimFYUBYz1viSBV4K-sg4sOOBmi8R=bupr1Hzm3RQ@mail.gmail.com"> <meta http-equiv="content-type" content="text/html; charset=UTF-8"> <div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details: <div>1. No. of participants (maximum expected), and</div> <div>2. Your maximum budget.</div> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a href="mailto:markos@c2o.pro.br" moz-do-not-send="true" class="moz-txt-link-freetext">markos@c2o.pro.br</a>> wrote:<br> </div> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer" target="_blank" moz-do-not-send="true">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" moz-do-not-send="true" class="moz-txt-link-freetext">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a class="moz-txt-link-abbreviated" href="http://www.diybio.org">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" moz-do-not-send="true" class="moz-txt-link-freetext">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CANpz%3DfE7PJimFYUBYz1viSBV4K-sg4sOOBmi8R%3Dbupr1Hzm3RQ%40mail.gmail.com?utm_medium=email&utm_source=footer" moz-do-not-send="true">https://groups.google.com/d/msgid/diybio/CANpz%3DfE7PJimFYUBYz1viSBV4K-sg4sOOBmi8R%3Dbupr1Hzm3RQ%40mail.gmail.com</a>.<br> </blockquote> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-8882009945569390712023-08-16T12:08:00.001-07:002023-08-16T12:08:45.663-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<p>Hi Kit,</p> <p>Please any recipe with the amount? How many grams of each component?</p> <p>I don't have an autoclave so I would use a pressure cooker.</p> <p>Could I use whey protein instead of tryptone?</p> <p>Thanks,</p> <p>Markos<br> </p> <div class="moz-cite-prefix">Em 16/08/2023 00:26, Kit Martens escreveu:<br> </div> <blockquote type="cite" cite="mid:551b8dce-d604-4476-a277-655f5c400d95n@googlegroups.com"> <meta http-equiv="content-type" content="text/html; charset=UTF-8"> <div>you could also use kitchen agar, nutritional yeast, salt, and tryptone (if you can source it) to make some LB plates - just mix everything together and autoclave it, pour some plates and store them in the fridge until you're ready to use them. Give the kids each three plates. Have the kids breathe on or touch two of them, and then add a teaspoon of bleach to one of the two. in a day you'll be able to see colonies on the plate with no bleach, and no colonies (hopefully) on the plate with bleach. It's a little more involved than the yeast version Simon suggested, but it might be a bit more engaging - it lets them go through an experiment and record results - plus get some exposure to agar plates - which are just a lot of fun and the colonies from just air and hands often end up being pretty and colourful.<br> </div> <div><br> </div> <br> <div class="gmail_quote"> <div dir="auto" class="gmail_attr">On Sunday, August 13, 2023 at 5:40:01 PM UTC-7 Simon Quellen Field wrote:<br> </div> <blockquote class="gmail_quote" style="margin: 0 0 0 0.8ex; border-left: 1px solid rgb(204, 204, 204); padding-left: 1ex;"> <div dir="ltr"> <div class="gmail_default" style="font-family:verdana,sans-serif;font-size:small">It is fairly easy to show the activity of yeast in a sugar water solution.</div> <div class="gmail_default" style="font-family:verdana,sans-serif;font-size:small">Add bleach, and the bubbles stop.</div> <div class="gmail_default" style="font-family:verdana,sans-serif;font-size:small">I suspect bubbling chlorine gas through it would have the same effect, but that is not something I would try with twelve-year-olds.</div> <div class="gmail_default" style="font-family:verdana,sans-serif;font-size:small"><br> </div> <div> <div dir="ltr" class="gmail_signature" data-smartmail="gmail_signature"> <div dir="ltr"> <div>-----</div> Get a free science project every week! "<a href="http://scitoys.com/newsletter.html" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=http://scitoys.com/newsletter.html&source=gmail&ust=1692136569508000&usg=AOvVaw0Pn9WNq_hufxpI8TkWaHnc" moz-do-not-send="true" class="moz-txt-link-freetext">http://scitoys.com/newsletter.html</a>"<br> <br> <a href="https://scitoys.com/books/" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://scitoys.com/books/&source=gmail&ust=1692136569508000&usg=AOvVaw3d6DV9Zd5SG1yW4j7i2Cg3" moz-do-not-send="true">Books by Simon Quellen Field</a><br> <div> <div style="color:rgb(34,34,34)"><font face="Roboto, RobotoDraft, Helvetica, Arial, sans-serif"><a href="https://www.paypal.com/cgi-bin/webscr?cmd=_s-xclick&hosted_button_id=EXYW3SNUCNSN4" style="color:rgb(17,85,204)" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://www.paypal.com/cgi-bin/webscr?cmd%3D_s-xclick%26hosted_button_id%3DEXYW3SNUCNSN4&source=gmail&ust=1692136569508000&usg=AOvVaw1JP4YNx9Xwu4q1cLfg1gY5" moz-do-not-send="true">Buy me a cookie</a></font></div> <div style="color:rgb(34,34,34)"> <div style="font-family:verdana,sans-serif"><a href="https://www.paypal.com/cgi-bin/webscr?cmd=_xclick&business=leven%40scitoys%2ecom&lc=US&item_name=Buy%20Me%20a%20Dozen%20Cookies&amount=12%2e00&currency_code=USD&button_subtype=services&no_note=0&bn=PP%2dBuyNowBF%3abtn_buynowCC_LG%2egif%3aNonHostedGuest" style="color:rgb(17,85,204)" target="_blank" rel="nofollow" data-saferedirecturl="https://www.google.com/url?hl=en&q=https://www.paypal.com/cgi-bin/webscr?cmd%3D_xclick%26business%3Dleven%2540scitoys%252ecom%26lc%3DUS%26item_name%3DBuy%2520Me%2520a%2520Dozen%2520Cookies%26amount%3D12%252e00%26currency_code%3DUSD%26button_subtype%3Dservices%26no_note%3D0%26bn%3DPP%252dBuyNowBF%253abtn_buynowCC_LG%252egif%253aNonHostedGuest&source=gmail&ust=1692136569508000&usg=AOvVaw2vMM64bQ9Ldr12n35tyj68" moz-do-not-send="true">Buy me a dozen cookies</a></div> </div> </div> </div> </div> </div> <br> </div> <br> <div class="gmail_quote"> <div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 5:14 PM Markos <<a href="" data-email-masked="" rel="nofollow" moz-do-not-send="true">mar...@c2o.pro.br</a>> wrote:<br> </div> </div> <div class="gmail_quote"> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer nofollow" target="_blank" data-saferedirecturl="https://www.google.com/url?hl=en&q=http://www.c2o.pro.br&source=gmail&ust=1692136569508000&usg=AOvVaw3KAi9YL5zth6_xIVyiyIWg" moz-do-not-send="true">www.c2o.pro.br</a><br> <br> </blockquote> </div> <div class="gmail_quote"> <blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" moz-do-not-send="true" class="moz-txt-link-freetext">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a class="moz-txt-link-abbreviated" href="http://www.diybio.org">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com" moz-do-not-send="true" class="moz-txt-link-freetext">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/551b8dce-d604-4476-a277-655f5c400d95n%40googlegroups.com?utm_medium=email&utm_source=footer" moz-do-not-send="true">https://groups.google.com/d/msgid/diybio/551b8dce-d604-4476-a277-655f5c400d95n%40googlegroups.com</a>.<br> </blockquote> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0tag:blogger.com,1999:blog-8908531747671358980.post-91527675703823996252023-08-16T08:17:00.000-07:002023-08-16T08:18:21.199-07:00Re: [DIYbio] Activity to demonstrate the disinfectant action of chlorine for young (12-16)<div dir="ltr">All good answers already. Before I share my suggestion / protocol, can you reply back with 2 details:<div>1. No. of participants (maximum expected), and</div><div>2. Your maximum budget.</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Sun, Aug 13, 2023 at 8:14 PM Markos <<a href="mailto:markos@c2o.pro.br">markos@c2o.pro.br</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left-width:1px;border-left-style:solid;border-left-color:rgb(204,204,204);padding-left:1ex">Hello,<br> <br> I'm organizing a citizen science course with diy activities about water <br> for young people aged between 12 and 16 years old.<br> <br> I want to include an activity to demonstrate the disinfectant action of <br> chlorine, and I need to find an activity that uses affordable materials <br> and culture media and a protocol that is easy for participants to <br> replicate with kitchen utensils.<br> <br> But as a Chemist I ask a help to suggest examples on web so I can save <br> time searching on Google.<br> <br> Thanks,<br> <br> Markos<br> <br> <a href="http://www.c2o.pro.br" rel="noreferrer" target="_blank">www.c2o.pro.br</a><br> <br> -- <br> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en" rel="noreferrer" target="_blank">https://groups.google.com/d/forum/diybio?hl=en</a><br> Learn more at <a href="http://www.diybio.org" rel="noreferrer" target="_blank">www.diybio.org</a><br> --- <br> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio%2Bunsubscribe@googlegroups.com" target="_blank">diybio+unsubscribe@googlegroups.com</a>.<br> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/7f44d614-3d18-85cc-f8dc-c25be3dfe9ae%40c2o.pro.br" rel="noreferrer" target="_blank">https://groups.google.com/d/msgid/diybio/7f44d614-3d18-85cc-f8dc-c25be3dfe9ae%40c2o.pro.br</a>.<br> </blockquote></div> <p></p> -- <br /> -- You received this message because you are subscribed to the Google Groups DIYbio group. 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For more options, visit this group at <a href="https://groups.google.com/d/forum/diybio?hl=en">https://groups.google.com/d/forum/diybio?hl=en</a><br /> Learn more at www.diybio.org<br /> --- <br /> You received this message because you are subscribed to the Google Groups "DIYbio" group.<br /> To unsubscribe from this group and stop receiving emails from it, send an email to <a href="mailto:diybio+unsubscribe@googlegroups.com">diybio+unsubscribe@googlegroups.com</a>.<br /> To view this discussion on the web visit <a href="https://groups.google.com/d/msgid/diybio/CANpz%3DfE7PJimFYUBYz1viSBV4K-sg4sOOBmi8R%3Dbupr1Hzm3RQ%40mail.gmail.com?utm_medium=email&utm_source=footer">https://groups.google.com/d/msgid/diybio/CANpz%3DfE7PJimFYUBYz1viSBV4K-sg4sOOBmi8R%3Dbupr1Hzm3RQ%40mail.gmail.com</a>.<br /> Sciencehttp://www.blogger.com/profile/02552051893681242957noreply@blogger.com0