On Wednesday, April 4, 2012 12:47:17 PM UTC-5, phillyj wrote:
On Wed, Apr 4, 2012 at 4:28 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> Anyone out there know enough biochemistry to suggest a way of safely polymerising health-store bought lysine to make polylysine?
>
From Japan: Food-grade PolyLysine Preservative $13/5g (Postage included?)http://www.wholesalegroup.com.
au/nata.html Not sure about the quality, etc.
I don't want to say that epsilon-poly-lysine won't work, but I know the attachment protocols I've read and used worked with alpha-poly-lysine. I know poly-ornithine and poly-arginine can be used to largely the same effect as poly-lysine. I would assume that random polypeptides would work to a modest degree. I'm sure some textbook or paper somewhere has a table of surface modifiers that have been tried and their respective effectiveness, but I don't (know that I) own it.
(I think that) Lysine will be relatively hard to polymerize cheaply/safely/easily de novo as it has two primary amines of similar reactivity. That is, when a-poly-lysine is produced in vitro, lysine precursors that have protected epsilon amines are used. I will say that the coatings I've done, 100 mg is A LOT of poly-lysine and a good portion of that (90%) is used to bias the equillibrium and drive the reaction rather than actually reacting.
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/BNbWRm4MhB4J.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment