Problem is, acidophilus isn't a primary yoghurt culture, AFAIK. It can be part of a yoghurt culture, but I suspect only as a minority share. The two main cultures in what's officially termed "Yoghurt" are S.salivarius thermophilus and L.delbrueckii bulgaricus.
The significance being that if your transformed culture isn't a significant player, it's also not gonna delivery enough light to notice. :/
Nathan McCorkle <nmz787@gmail.com> wrote:
>I just sent a paper regarding the lab protocol for L. acidophipus, 2
>messages ago.
>On Apr 5, 2012 8:31 AM, "Petfixer71" <afrishman71@hotmail.com> wrote:
>
>> Im sure you have seen this:
>>
>> http://www.indiebiotech.com/?p=164#more-164
>>
>> If anyone knows were I can get the specific detailed lab protocol I
>would
>> appreciate it.
>>
>> On Saturday, March 31, 2012 10:38:12 AM UTC-4, medminus9 wrote:
>>>
>>> Hello all!
>>>
>>> I am working as an instructor to a HS iGEM team and as a part of
>their
>>> project I am helping them design bioluminescent yogurt. I have been
>through
>>> the previous thread and all the blog posts attached to the thread,
>but
>>> found that most of the ideas were lacking references and supportive
>>> literature. So, this is what i have proposed after going through the
>>> literature, your inputs and help will be much appreciated! :))
>>>
>>> - Acetaldehyde: Acetaldehyde can react with luciferase complex to
>>> produce bioluminescence. Since acetaldehyde is produced by
>lactobacillus
>>> and streptococcus thermophilus in abundance, it will be an ideal
>substrate
>>> for the luciferase to chew-on. It is also among the most abundant
>chemicals
>>> on Earth and will help reducing the size of our construct from
>luxCDABEG to
>>> luxABG.
>>>
>>>
>http://www.jbmb.or.kr/jbmb/**jbmb_files/[20-1]0204292121_**02600541.pdf<http://www.jbmb.or.kr/jbmb/jbmb_files/%5B20-1%5D0204292121_02600541.pdf>
>>>
>>>
>>> - Acidophilus: I am planning to work with acidophilus for the
>>> following reasons:
>>>
>>>
>>> 1. Peroxide tolerance: Is more tolerant to H202 in compare to
>>> bulgaricus and several other species. Since bioluminescence will
>require
>>> aerobic conditions, the yogurt bacteria's will produce plenty of
>peroxide.
>>> 2. Research: The bacteria has been thoroughly studied.
>>> 3. Cost: My friend is making it available to me for free. He has
>been
>>> doing some research before.
>>> 4. H202: Since acidophilus is H202 producing, the H202 can serve
>as
>>> an oxidizing agent required to form the flavin-peroxy
>intermediate. (Not
>>> sure!)
>>>
>>>
>http://www.ncbi.nlm.nih.gov/**pmc/articles/PMC84537/?tool=**pmcentrez<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC84537/?tool=pmcentrez>
>>>
>>>
>>> - NAD(P)H2.FMN oxidoreductase: It is found in some species of
>>> acidophilus. I can't find the literature for this, but I do
>happen to have
>>> read it somewhere. Also most of the L.casei species do have this
>enzyme so
>>> I believe we if required we could even add the particular enzyme
>to the
>>> construct.
>>>
>>> *Problems*:
>>>
>>> 1. Cathal in his blog post is favoring a linear transfer, but
>most of
>>> the literature is strongly against transformation with linear
>DNA. So, now
>>> I am confused in choosing between a plasmid transfer with
>chemical/ natural
>>> competence or a linear transfer?
>>> 2. I am searching for a* constitutive promoter* for the purpose
>and
>>> would really appreciate any help with this.
>>> 3. Is there anything which I am missing or might have not looked
>>> over. Your suggestions please! :)
>>>
>>>
>>> --
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