recipe: http://www-cyanosite.bio.purdue.edu/media/table/f2.html
Also, I would look into getting the cheap mass-packs from florida aqua farms/some other aquaculture supplier (I would hook you up with where I bought it from, but the bottle is in MA...I will check when I find it).
IMO, it is almost never worth it to piece together media by hand, especially if you need small amounts. I had to make "marine broth" once, and we went nuts trying to find all kinds of crap, from strontium salts to buffers. Each stock chemical we used was easily $90+ for the size of the bottle, but we ended up using milligrams (This is when I learned milligrams were small). It was only feasible because we had it all on hand.
--A
On Mon, Aug 27, 2012 at 4:43 PM, Sebastian S. Cocioba <scocioba@gmail.com> wrote:
Anyone have a recipe for commercial broth on hand? Maybe the paper that produced said broth might shine some light on the dino's needs. My hunch is copper content since mg has a slightly higher than average cupric quantity :)
Sebastian S CociobaCEO & FounderNew York Botanics, LLCSent via Mobile E-MailMy friend and I had read somewhere that you could with miracle grow, but our initial attempt caused our dinoflagellates to die sooner than those in the normal broth they came in.
That said we didn't investigate further. Designing an experiment that investigates some of the recommended approaches would be useful.
Cheers,
Robert Osazuwa Ness
On Monday, August 27, 2012 5:16:11 AM UTC-4, Loïc wrote:Hi everybody,--
Does anybody knows a DIY liquid media for growing bioluminescence algae like Pyrocystis lunula.
I grow this algae with a standard laboratory media, but it's too expensive for massive growing.
Thank you for yours answers.
.
Thanks
Best regards
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