Or, you can try to isolate biosurfactant-producing microorganisms and use their surfactants. Many commercial detergents include surfactants of microbial origin. The problem would be isolate the microorganism of interest (and non-pathogenic). Probably, starting with a minimal medium adding diesel as sole carbon source, 1-2 weeks incubation, isolation in rich medium and test surfactant properties of isolates in pure cultures.
If you know any place affected by oil, diesel, gasoline,... spill or a soil close to a petrol station, are good source samples to get faster and higher output.
El martes, 6 de noviembre de 2012 01:47:40 UTC+11, Cathal escribió:
Problem: most cellular lipids are, AFAIK, *phospho*lipids. That is, one--
end is conjugated to a highly charged phosphate group, which allows the
lipids to form soluble micelles instead of clumping into droplets of
insoluble oil that can be separated by burette.
To extract "oil" from cellular paste, you might be able to find an
enzyme that cleaves this phosphate group from the phospholipids, leading
to a mix of insoluble fatty acids and free phosphate; this would give
you oil for your saponification (soap) and free phosphate as a
fertiliser, although I wouldn't recommend simply adding it to the soil
as it would be far too strong and prone to runoff. Feeding the
phosphate-rich byproduce to aero/hydro-ponic plants, or simply letting
it get digested into more complex cellular matter by bacteria or fungi
before use as compost, might be wiser.
On 05/11/12 02:24, Ulysses1994XF04 wrote:
> That seems like a pretty good idea! But what about during fall and winter?
> I was thinking bacteria and fungus because I could just grow it indoors all
> year round; perhaps if I built some indoor hydroponic system, I could
> plants indoors as well. What are some good, small oily plants?
>
> Also, how would you effectively harvest their oils and lipids? I was
> thinking maybe column chromatography? Maybe buy a buret online and some
> silica gel, pack it and run it through to separate oils from the cell
> components? Would that work? What kind of mobile phase would I need?
>
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