[DIYbio] Re: [Biohackers] Open source centrifuge!

In most cases, a 2000g, 6000 rpm minifuge is all you need for mini preps and pelleting DNA. Galaxy VWR minifuge for this is $422, brand new, from VWR. This kit is $260. You can pick up minifuges at used resellers for <$50. A basic Dremel tool is about $50. A replacement rotor for the galaxy microfuge (hint, hint) is $70.

To Patrik's point: how much is your eyesight worth? Be careful out there. When making plastic covers, use highly resistant materials, like polycarbonate laminates (e.g. MAKROLON). Metal rotors are probably a better idea than plastic ones. IMO, the brand new minifuge is not so expensive and has clearly traceable product liability. DIY in this case might be a great learning tool, if by "losing an eye" you learn what not to do.

This has been your "Important safety tip there, Egan" of the day.

Matt

On Thursday, September 26, 2013, Patrik D'haeseleer wrote:

I would also want to see what happens to that thin acrylic cover when a fully laden tube ejects from the rotor at full speed. High speed centrifuges are explosion-prone devices, and need to be tested as such. Flying shards of acrylic are no fun...

The typical recommendation for using the dremelfuge are to hold it inside a metal cooking pot - at least that will give you some serious protection.

Patrik

On Thu, Sep 26, 2013 at 10:17 AM, Craig Rouskey <craigrouskey@gmail.com> wrote:

Thanks Jenny! Typically for DNA work centrifuge speeds need to be approx. 13000rpm/18000xg

—
Craig J. Rouskey
contact@craigrouskey.com
http://www.craigrouskey.com

On Thu, Sep 26, 2013 at 1:15 AM, Jenny Ryan <tunabananas@gmail.com> wrote:

Via @BiologiGaragen: New open source centrifuge
http://t.co/otDqV44UER http://t.co/54l7f5DNOt
Shared via TweetCaster

_______________________________________________
Biohackers mailing list
Biohackers@lists.sudoroom.org
http://lists.sudoroom.org/listinfo/biohackers

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2BRBL9k8bVp%3Doguh5t4Ea%3DM2wZJ%3D5BbKS-G0JkNxTia1uMmp3w%40mail.gmail.com.
For more options, visit https://groups.google.com/groups/opt_out.