Re: [DIYbio] Re: Requesting your thoughts on a yogurt-based nutraceutical platform

Actually, it looks like there's a tyrosine ammonia-lyase in tea, strawberries and corn as well, and phenylalanine/tyrosine ammonia-lyase in soy, sunflower, sorghum and wheat:

http://www.brenda-enzymes.org/php/flat_result.php4?ecno=4.3.1.23&organism_list=&Suchword=&UniProtAcc=#ORGANISM
http://www.brenda-enzymes.org/php/flat_result.php4?ecno=4.3.1.25&organism_list=&Suchword=&UniProtAcc=#ORGANISM

Not sure that the genes for these activities have been annotated in all of them, but there should not be a shortage of TAL sequences from food plants to play with.

Hm... BRENDA actually also lists PAL4a and PAL5a from Vitis amurensis as being tyrosine ammonia-lyases. Haven't tracked down where that annotation comes from, bu if true, you might be able to do your design purely based on (different species of) grape.

Patrik



On Tuesday, October 8, 2013 6:29:56 PM UTC-7, Richard Yu wrote:

Exactly Nathan. Though we can probably dig for a food-grade version of a TAL, or perhaps even mutate the grape PAL or HAL to get to mostly TAL function as an intermediate stage to not rouse the pitchforks.

Rich

On Tue, Oct 8, 2013 at 1:17 AM, Nathan McCorkle <nmz...@gmail.com> wrote:

On Tue, Oct 8, 2013 at 1:15 AM, Patrik D'haeseleer <pat...@gmail.com> wrote:
> By the way - it looks like you may be able to do away with that pesky
> unstable, membrane-bound, P450-interacting C4H enzyme altogether! The paper
> I quoted earlier (Combinatorial Biosynthesis of Non-bacterial and Unnatural
> Flavonoids, Stilbenoids and Curcuminoids by Microorganisms) also mentions
> that PAL from R. rubra

...
except they want to use only l.spp.bulgaricus and grape genes to calm
the masses.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/brrF6l0sc1M/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.

To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9KsPtxm5ptNb2a7hTZkiZXOzJ6CiprUjRJKUzhmy%2BkNvQ%40mail.gmail.com.

For more options, visit https://groups.google.com/groups/opt_out.

--

Richard Yu

Radiant Genomics

5858 Horton St Ste 375

Emeryville, CA 94608

510-288-8776

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/27544d50-95dc-4d9f-88ea-f65d172b58bf%40googlegroups.com.
For more options, visit https://groups.google.com/groups/opt_out.