Re: [DIYbio] Covid vaccine.

I think at this stage, the input that computational modeling could provide may show if any part of the sequence to be used can sufficiently bind the virus. If binding does occur computationally, then it might be worth it to proceed with testing such sequence.

On Mon, Apr 6, 2020 at 11:25 AM Bryan Jones <bryanjjones@gmail.com> wrote:

You might get a different response if you use a portion than if you used whole protein. Specifically, you are probably less likely to produce neutralizing antibodies from a short peptide. Ideally, you'd probably want to leave off the membrane anchor part of the protein. Maybe a soluble construct like the one used here: http://dx.doi.org/10.1016/j.cell.2020.02.058  It might be important to get human-like glycosylation, so E. coli or Sacromyces might not be the best expression system.

On Sun, Apr 5, 2020, 19:38 <djwrister@gmail.com> wrote:

I think we are desperate enough. Let's do it. Step one. Do we need the entire spike protein or just a portion for antibodies?

Sent from my iPhone

> On Apr 5, 2020, at 4:02 PM, Zonie deep <deckeraaa@gmail.com> wrote:
>
> You know if we are desperate enough it would be quite easy to make your own purified recombinant covid spike protein in bacteria. This could be used for a vaccine without too much regulatory. Protein could be made in a few weeks. Need to be sterile of course, dosage worked out. I've only immunized rabbits with these types of preps. Always get good antibodies,no harm to rabbits.
>
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