Re: [DIYbio] Alive in 2025

Very cool.  I think the electroporator would be great for amateur labs, colleges ,and schools.  Better transformation efficiency than calcium chloride is the selling point? 

RV/Off Grid / Van Life probably has a larger customer base though for sure.  It's definitely a "fad" on instagram (I follow some accounts) but I feel like there's a big difference between wealthy people who live in a very nice Mercedes sprinter and have somewhere to go back to, vs someone living out of their car because of desperation.  Either way, off-grid living hardware sounds very interesting no matter who you're helping. 

On Tue, Jan 21, 2025 at 1:01 PM 'John Griessen' via DIYbio <diybio@googlegroups.com> wrote:
On 1/21/25 09:59, Dakota Hamill wrote:
> What are you working on in this new year?

Microscope repair and building up cash to continue the culture shock electroporator plastic case project.
I got a 3D filament printer for its frame and guts -- it's a leapfrog creatr, so it will benefit from open controls.
I might be able to get a CNC circuit board prototyper at auction tomorrow -- that will help do new designs.

There was little interest in a mostly working electroporator so my new designs will be about remote living off grid in huts or RVs
instead of bio lab gear, unless it's a prepaid sure thing.  But, I am going to slowly finish the  culture shock electroporator
plastic case as a lower priority -- some RV/off-grid products may launch before that is beta tested.


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[DIYbio] Free fragment analysis/capillary eletrophoresis data analysis software

Recently, I've released FragalyseQt v 0.4, codenamed "Jeffreys", now with support for fragment sizing! It is a free (free as in "freedom", not in "free beer" - Richard Matthew Stallman) software for fragment analysis/capillary electrophoresis data handling.
photo_2024-09-01_17-45-51.jpg
FragalyseQt supports sizing using either linear, cubic or 5th degree spline or LSQ of 2nd, 3rd or 5th order, can handle ABIF files from virtually every CE machine, even from ancient ABI 310 with ancient version of ABIF, not described in specifications published by ABI or RapidHIT ID, implementing only very limited subset of ABIF, or even HID files from ABI 3500 HID. It can export analysis results in CSV, or if internal analysis results are available (for files obtained using ABI 3500, SeqStudio, SeqStudio Flex) - it can export them too.

FragalyseQt is non-invasive analysis tool, leaving analysed files in exactly the same condition they were before analysis.

It is highly portable and proven to work at: Linux, macOS, Windows (Win7+), OpenBSD, FreeBSD, NetBSD and uses only 200 MB or RAM. It also supports Non-Latin run anmes and different interface languages as: English, Russian, French, Romanian, Bulgarian, Ukrainian. Language is set automatically using OS language settings.

Software is licensed under AGPL v3.

At the screenshot you may observe calculaed sizes of 
GlobalFiler allelic ladder peaks (in program window) and expected sizes with accepted deviations (in LibreOffice).

FragalyseQt source code is available at: https://github.com/Dorif/fragalyseqt

Release 0.4 is available at: https://github.com/Dorif/fragalyseqt/releases/tag/jeffreys_initial

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