March 2012 | Science

[Virtual University Of PakistanStudent Cafe] .::VULMSIT::.eNoxel Good Bye MSCHOTAMASTER

7:32 PM |

Aslam u Alakum!

Friends Finally I have completed my degree and got job in lahore. So Now it is time to say Good Bye. AnyOne who got disterbance etc through my behavior or mistakes Forgive me I have forgived all.Anyone want any help in .net envoirment frequently call me on 03456550981 or send email on mschotamaster@gmail.com

Remember me in ur prayers

Allah Hafiz

Regards

mschotamaster

--
Virtual University of Pakistan*** IT n CS Blog
================================
http://www.geniusweb.tk

http://itncs.tk

You received this message because you are subscribed to the Google
Groups "vulms" group.
To post to this group, send email to vulmsit@googlegroups.com
To unsubscribe from this group, send email to
vulmsit+unsubscribe@googlegroups.com
For more options, visit this group at
http://groups.google.com/group/vulmsit?hl=en?hl=en

--
Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 3/31/2012 07:32:00 PM

--
You received this message because you are subscribed to the Google Groups "VU Computer Science" group.
To post to this group, send email to vu-computer-science@googlegroups.com.
To unsubscribe from this group, send email to vu-computer-science+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/vu-computer-science?hl=en.

Read User's Comments(0)

[DIYbio] Re: [diybio-boston] diybio open hardware community?

6:23 PM |

There's now DIY hardware communities which promote everything as open (http://sharism.org/en/presents) ... they call it "gifts of knowledge". These operate a copyleft hardware design shop (http://en.qi-hardware.com/wiki/Ben_NanoNote) which document everything from board schematics, build process to Bill of Materials. Microscopy with complex lens arrangements might be a little tricky for them unless you want lens on a fibre. Costs are reduced through a book build commit to minimum size orders before ordering components and production.

Lawrence
http://www.linkedin.com/in/drllau

On Sunday, 1 April 2012 06:24:55 UTC+13, Bryan Bishop wrote

Date: Sat, Mar 31, 2012 at 11:50 AM
Subject: Re: [diybio-boston] diybio open hardware community?
To: diybio-boston@googlegroups.com

I'm actually looking at building my own modular microscopy system from scratch. Modifying premade microscopes is definitely cheaper, but much less flexible if you want to add filters/optics/etc. for fancy imaging techniques. I'm starting my design by building a research-quality scope first and then figuring out how to minimize the costs to make it more affordable.

My DIY fluorescence microscope probably runs around $2k is my guess -- about an order of magnitude cheaper than competing microscopes by Olympus/Nikon/etc., but still too expensive for individuals.
http://asymptoticdesign.wordpress.com/2012/03/10/diy-scope/

I'm also workign on software to go with it:
http://asymptoticdesign.wordpress.com/2012/03/29/openscope-software-v1-0/

I'm currently working on documenting the fluorescence imaging and adding a confocal microscopy extension to it, and then it's going to be all about how to minimize costs.

nathan

On Sat, 31 Mar 2012, Christopher Saenz wrote:

No but let's get on it. I am relatively new to stuff at sprout. I went a couple of times but then got busy finishing my degree. I just graduated and now can dedicate some time to this stuff. I polished up the epi the last time i was there. what were you looking at doing with microscopy?

chris

On Mar 31, 2012, at 12:05 PM, nathan lachenmyer wrote:

Does anyone know of any open hardware communities; especially ones that are diybio oriented? I'd like to find a community to both share and collaborate on open hardware tools for biology, but most of the openhardware communities I can find aren't really science oriented.

I've been working on a few projects (in particular, open source microscopy) and would be interested to find other people to check out/test/etc. my projects with.

Thanks,

Nathan

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
- Bryan
http://heybryan.org/
1 512 203 0507

On Sunday, 1 April 2012 06:24:55 UTC+13, Bryan Bishop wrote:

Date: Sat, Mar 31, 2012 at 11:50 AM
Subject: Re: [diybio-boston] diybio open hardware community?
To: diybio-boston@googlegroups.com

I'm actually looking at building my own modular microscopy system from scratch. Modifying premade microscopes is definitely cheaper, but much less flexible if you want to add filters/optics/etc. for fancy imaging techniques. I'm starting my design by building a research-quality scope first and then figuring out how to minimize the costs to make it more affordable.

My DIY fluorescence microscope probably runs around $2k is my guess -- about an order of magnitude cheaper than competing microscopes by Olympus/Nikon/etc., but still too expensive for individuals.
http://asymptoticdesign.wordpress.com/2012/03/10/diy-scope/

I'm also workign on software to go with it:
http://asymptoticdesign.wordpress.com/2012/03/29/openscope-software-v1-0/

I'm currently working on documenting the fluorescence imaging and adding a confocal microscopy extension to it, and then it's going to be all about how to minimize costs.

nathan

On Sat, 31 Mar 2012, Christopher Saenz wrote:

No but let's get on it. I am relatively new to stuff at sprout. I went a couple of times but then got busy finishing my degree. I just graduated and now can dedicate some time to this stuff. I polished up the epi the last time i was there. what were you looking at doing with microscopy?

chris

On Mar 31, 2012, at 12:05 PM, nathan lachenmyer wrote:

Does anyone know of any open hardware communities; especially ones that are diybio oriented? I'd like to find a community to both share and collaborate on open hardware tools for biology, but most of the openhardware communities I can find aren't really science oriented.

I've been working on a few projects (in particular, open source microscopy) and would be interested to find other people to check out/test/etc. my projects with.

Thanks,

Nathan

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
- Bryan
http://heybryan.org/
1 512 203 0507

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/2FFP_BOpW6EJ.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

Read User's Comments(0)

Re: [DIYbio] Bioluminescent yogurt (Again!)

3:37 PM |

googling "electroporation acidophilus" gives lots of results, the first says electroporation yieled "7.4 × 10⁶ transformants per μg of plasmid DNA under optimized conditions"... so basically you should have no problems transforming small circular or linear DNA.

I'm also on the genomic integration is better" boat. Once its in there, as long as the genes are toxic or draining on the cell. constitutive promoters could 'drain'/strain the cell because of a constant amino acid demand... so maybe you want a low expression constitutive, or something thats only induced. Maybe lac promoter could work, so induction at least tapers off as incubation time increases.

On Sat, Mar 31, 2012 at 10:38 AM, medminus9 <harshsethia1989@gmail.com> wrote:

Hello all!

I am working as an instructor to a HS iGEM team and as a part of their project I am helping them design bioluminescent yogurt. I have been through the previous thread and all the blog posts attached to the thread, but found that most of the ideas were lacking references and supportive literature. So, this is what i have proposed after going through the literature, your inputs and help will be much appreciated! :))

Acetaldehyde: Acetaldehyde can react with luciferase complex to produce bioluminescence. Since acetaldehyde is produced by lactobacillus and streptococcus thermophilus in abundance, it will be an ideal substrate for the luciferase to chew-on. It is also among the most abundant chemicals on Earth and will help reducing the size of our construct from luxCDABEG to luxABG.

http://www.jbmb.or.kr/jbmb/jbmb_files/[20-1]0204292121_02600541.pdf
Acidophilus: I am planning to work with acidophilus for the following reasons:

Peroxide tolerance: Is more tolerant to H202 in compare to bulgaricus and several other species. Since bioluminescence will require aerobic conditions, the yogurt bacteria's will produce plenty of peroxide.

Research: The bacteria has been thoroughly studied.
Cost: My friend is making it available to me for free. He has been doing some research before.

H202: Since acidophilus is H202 producing, the H202 can serve as an oxidizing agent required to form the flavin-peroxy intermediate. (Not sure!)
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC84537/?tool=pmcentrez

NAD(P)H2.FMN oxidoreductase: It is found in some species of acidophilus. I can't find the literature for this, but I do happen to have read it somewhere. Also most of the L.casei species do have this enzyme so I believe we if required we could even add the particular enzyme to the construct.

Problems:
Cathal in his blog post is favoring a linear transfer, but most of the literature is strongly against transformation with linear DNA. So, now I am confused in choosing between a plasmid transfer with chemical/ natural competence or a linear transfer?

I am searching for a constitutive promoter for the purpose and would really appreciate any help with this.
Is there anything which I am missing or might have not looked over. Your suggestions please! :)

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/-6eZAIv4eHUJ.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

Read User's Comments(0)

Re: [DIYbio] Fwd: [diybio-boston] diybio open hardware community?

10:39 AM |

On Sat, Mar 31, 2012 at 12:32 PM, Simon Quellen Field <sfield@scitoys.com> wrote:

I am also working on inexpensive home-brew microscopy.

Well? Let's see it? By see I mean plans, but pics are cool too...

- Bryan
http://heybryan.org/
1 512 203 0507

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

Read User's Comments(0)

Re: [DIYbio] Fwd: [diybio-boston] diybio open hardware community?

10:32 AM |

I am also working on inexpensive home-brew microscopy.

-----

Get a free science project every week! "http://scitoys.com/newsletter.html"

On Sat, Mar 31, 2012 at 10:24 AM, Bryan Bishop <kanzure@gmail.com> wrote:

From: nathan lachenmyer <scottnla@mit.edu>
Date: Sat, Mar 31, 2012 at 11:50 AM
Subject: Re: [diybio-boston] diybio open hardware community?
To: diybio-boston@googlegroups.com

I'm actually looking at building my own modular microscopy system from scratch. Modifying premade microscopes is definitely cheaper, but much less flexible if you want to add filters/optics/etc. for fancy imaging techniques. I'm starting my design by building a research-quality scope first and then figuring out how to minimize the costs to make it more affordable.

My DIY fluorescence microscope probably runs around $2k is my guess -- about an order of magnitude cheaper than competing microscopes by Olympus/Nikon/etc., but still too expensive for individuals.
http://asymptoticdesign.wordpress.com/2012/03/10/diy-scope/

I'm also workign on software to go with it:
http://asymptoticdesign.wordpress.com/2012/03/29/openscope-software-v1-0/

I'm currently working on documenting the fluorescence imaging and adding a confocal microscopy extension to it, and then it's going to be all about how to minimize costs.

nathan

On Sat, 31 Mar 2012, Christopher Saenz wrote:

No but let's get on it. I am relatively new to stuff at sprout. I went a couple of times but then got busy finishing my degree. I just graduated and now can dedicate some time to this stuff. I polished up the epi the last time i was there. what were you looking at doing with microscopy?

chris

On Mar 31, 2012, at 12:05 PM, nathan lachenmyer wrote:

Does anyone know of any open hardware communities; especially ones that are diybio oriented? I'd like to find a community to both share and collaborate on open hardware tools for biology, but most of the openhardware communities I can find aren't really science oriented.

I've been working on a few projects (in particular, open source microscopy) and would be interested to find other people to check out/test/etc. my projects with.

Thanks,

Nathan

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
- Bryan
http://heybryan.org/
1 512 203 0507

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

Read User's Comments(0)

[DIYbio] Fwd: [diybio-boston] diybio open hardware community?

10:24 AM |

From: nathan lachenmyer <scottnla@mit.edu>

Date: Sat, Mar 31, 2012 at 11:50 AM
Subject: Re: [diybio-boston] diybio open hardware community?
To: diybio-boston@googlegroups.com

I'm actually looking at building my own modular microscopy system from scratch. Modifying premade microscopes is definitely cheaper, but much less flexible if you want to add filters/optics/etc. for fancy imaging techniques. I'm starting my design by building a research-quality scope first and then figuring out how to minimize the costs to make it more affordable.

My DIY fluorescence microscope probably runs around $2k is my guess -- about an order of magnitude cheaper than competing microscopes by Olympus/Nikon/etc., but still too expensive for individuals.
http://asymptoticdesign.wordpress.com/2012/03/10/diy-scope/

I'm also workign on software to go with it:
http://asymptoticdesign.wordpress.com/2012/03/29/openscope-software-v1-0/

I'm currently working on documenting the fluorescence imaging and adding a confocal microscopy extension to it, and then it's going to be all about how to minimize costs.

nathan

On Sat, 31 Mar 2012, Christopher Saenz wrote:

No but let's get on it. I am relatively new to stuff at sprout. I went a couple of times but then got busy finishing my degree. I just graduated and now can dedicate some time to this stuff. I polished up the epi the last time i was there. what were you looking at doing with microscopy?

chris

On Mar 31, 2012, at 12:05 PM, nathan lachenmyer wrote:

Does anyone know of any open hardware communities; especially ones that are diybio oriented? I'd like to find a community to both share and collaborate on open hardware tools for biology, but most of the openhardware communities I can find aren't really science oriented.

I've been working on a few projects (in particular, open source microscopy) and would be interested to find other people to check out/test/etc. my projects with.

Thanks,

Nathan

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
You received this message because you are subscribed to the Google Groups "diybio-boston" group.
To post to this group, send email to diybio-boston@googlegroups.com.
To unsubscribe from this group, send email to diybio-boston+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio-boston?hl=en.

--
- Bryan
http://heybryan.org/
1 512 203 0507

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

Read User's Comments(0)

[Virtual University Of PakistanStudent Cafe] .::VULMSIT::.eNoxel Girlfriend and Friend ........... hahahahahhahahaha

10:10 AM |

G/Friend: Plz Don't Drink & Drive, Accident Bohot
Ho Rahe Hain Aajkal.. ..

Boy: Thanks Baby, You're So Caring......

Friend: Plz Don't Drink & Drive, Accident Bohot
Ho Rahe Hain Aajkal....

Boy: Pata Hai Beta, Apne Baap Ko Mat Sikha..!!!

The One & Only .......... IRFAN.

Italy.

http://groups.yahoo.com/group/p_se_poetry

--
Virtual University of Pakistan*** IT n CS Blog
================================
http://www.geniusweb.tk

http://itncs.tk

You received this message because you are subscribed to the Google
Groups "vulms" group.
To post to this group, send email to vulmsit@googlegroups.com
To unsubscribe from this group, send email to
vulmsit+unsubscribe@googlegroups.com
For more options, visit this group at
http://groups.google.com/group/vulmsit?hl=en?hl=en

--
Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 3/31/2012 10:10:00 AM

--
You received this message because you are subscribed to the Google Groups "VU Computer Science" group.
To post to this group, send email to vu-computer-science@googlegroups.com.
To unsubscribe from this group, send email to vu-computer-science+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/vu-computer-science?hl=en.

Read User's Comments(0)

Re: [DIYbio] Re: query from PBS NewsHour

7:59 AM |

On Mar 31, 2012 5:01 AM, "Nathan McCorkle" <nmz787@gmail.com> wrote:
>
> On Sat, Mar 31, 2012 at 3:11 AM, Joseph Jackson <joseph.jackson@gmail.com> wrote:
> > We will eventually get to the promised land of synthetic biology, nanotech,
> > etc etc but in the mean time, a lot of people need to take a damn chill
> > pill.
>
> Thumbs Up to that.

yes, well said.

Jason

>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics
>
> --
> You received this message because you are subscribed to the Google Groups "DIYbio" group.
> To post to this group, send email to diybio@googlegroups.com.
> To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
> For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

Read User's Comments(0)

[DIYbio] Bioluminescent yogurt (Again!)

7:38 AM |

Hello all!

I am working as an instructor to a HS iGEM team and as a part of their project I am helping them design bioluminescent yogurt. I have been through the previous thread and all the blog posts attached to the thread, but found that most of the ideas were lacking references and supportive literature. So, this is what i have proposed after going through the literature, your inputs and help will be much appreciated! :))

Acetaldehyde: Acetaldehyde can react with luciferase complex to produce bioluminescence. Since acetaldehyde is produced by lactobacillus and streptococcus thermophilus in abundance, it will be an ideal substrate for the luciferase to chew-on. It is also among the most abundant chemicals on Earth and will help reducing the size of our construct from luxCDABEG to luxABG.

http://www.jbmb.or.kr/jbmb/jbmb_files/[20-1]0204292121_02600541.pdf

Acidophilus: I am planning to work with acidophilus for the following reasons:

Peroxide tolerance: Is more tolerant to H202 in compare to bulgaricus and several other species. Since bioluminescence will require aerobic conditions, the yogurt bacteria's will produce plenty of peroxide.
Research: The bacteria has been thoroughly studied.
Cost: My friend is making it available to me for free. He has been doing some research before.
H202: Since acidophilus is H202 producing, the H202 can serve as an oxidizing agent required to form the flavin-peroxy intermediate. (Not sure!)

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC84537/?tool=pmcentrez

NAD(P)H2.FMN oxidoreductase: It is found in some species of acidophilus. I can't find the literature for this, but I do happen to have read it somewhere. Also most of the L.casei species do have this enzyme so I believe we if required we could even add the particular enzyme to the construct.

Problems:

Cathal in his blog post is favoring a linear transfer, but most of the literature is strongly against transformation with linear DNA. So, now I am confused in choosing between a plasmid transfer with chemical/ natural competence or a linear transfer?
I am searching for a constitutive promoter for the purpose and would really appreciate any help with this.
Is there anything which I am missing or might have not looked over. Your suggestions please! :)

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/-6eZAIv4eHUJ.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

Read User's Comments(0)

[DIYbio] DIYbio and Bioethics {entering soft-science}

6:21 AM |

I was wondering if anyone is a hobbyist philosopher(or related, not
sure the name) who is studying Bioethics (the whole field rather than
DIYbio only). Why should DIYbio be confined to "hard" science like
labwork or in-silico? Is anyone, as a hobby, thinking and writing on
morals and ethics in biology and other science fields?

***Sorry if my words are not well-chosen, e.g. "soft-science",
"confined", etc. I hope my point is understandable.

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

Read User's Comments(0)

[Virtual University Of PakistanStudent Cafe] ..::VU-Pink::.. ````Yesterday Daily Quran and Hadith 30 March 2012...````

5:24 AM |

IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

Note : If Quran and Hadith is not viewable CLICK HERE to Read Today Quran and Hadith.

Visit
www.quranurdu.com
www.tafheemonline.com
www.quranenglish.com
www.daroos.com
www.islamicrishta.com

"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""

*~*$H!N!NG $T@R*~*

SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

--
...:::Group Rules:::...
http://groups.google.com.pk/group/vu-pink/web/group-rules
^^^^^^
You received this message because you are subscribed to the Google
Groups "vu pink" group.
To post to this group, send email to vu-pink@googlegroups.com
To unsubscribe from this group, send email to
vu-pink+unsubscribe@googlegroups.com
For more options, visit this group at
http://groups.google.com.pk/group/vu-pink?hl=en

--
Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 3/31/2012 05:22:00 AM

--
You received this message because you are subscribed to the Google Groups "VU Computer Science" group.
To post to this group, send email to vu-computer-science@googlegroups.com.
To unsubscribe from this group, send email to vu-computer-science+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/vu-computer-science?hl=en.

Read User's Comments(0)

[Virtual University Of PakistanStudent Cafe] [Pak Youth] ````Yesterday Daily Quran and Hadith 30 March 2012...````

5:22 AM |

IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

Note : If Quran and Hadith is not viewable CLICK HERE to Read Today Quran and Hadith.

Visit
www.quranurdu.com
www.tafheemonline.com
www.quranenglish.com
www.daroos.com
www.islamicrishta.com

"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""

*~*$H!N!NG $T@R*~*

SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

--
You received this message because you are subscribed to the Google Groups "Pak Youth" group.
To post to this group, send email to pak-youth@googlegroups.com.
To unsubscribe from this group, send email to pak-youth+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/pak-youth?hl=en.

--
Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 3/31/2012 05:22:00 AM

--
You received this message because you are subscribed to the Google Groups "VU Computer Science" group.
To post to this group, send email to vu-computer-science@googlegroups.com.
To unsubscribe from this group, send email to vu-computer-science+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/vu-computer-science?hl=en.

Read User's Comments(0)

[Virtual University Of PakistanStudent Cafe] ~~νυ ѕтυ∂у мαηια~~ ````Daily Quran and Hadith 31 March 2012...````

5:05 AM |

IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

Note : If Quran and Hadith is not viewable CLICK HERE to Read Today Quran and Hadith.

Visit
www.quranurdu.com
www.tafheemonline.com
www.quranenglish.com
www.daroos.com
www.islamicrishta.com

"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""

*~*$H!N!NG $T@R*~*

SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

--
Don't share personal phone number etc.
To post to this group, send email to vustudymania@googlegroups.com
For More Data Please Visit Our Official Website www.vubaba.com to get all the data you need
http://www.vubaba.com

http://groups.google.com/group/vustudymania

--
Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 3/31/2012 05:02:00 AM

--
You received this message because you are subscribed to the Google Groups "VU Computer Science" group.
To post to this group, send email to vu-computer-science@googlegroups.com.
To unsubscribe from this group, send email to vu-computer-science+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/vu-computer-science?hl=en.

Read User's Comments(0)

[Virtual University Of PakistanStudent Cafe] ..::VU-Pink::.. ````Daily Quran and Hadith 31 March 2012...````

5:03 AM |

IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

Note : If Quran and Hadith is not viewable CLICK HERE to Read Today Quran and Hadith.

Visit
www.quranurdu.com
www.tafheemonline.com
www.quranenglish.com
www.daroos.com
www.islamicrishta.com

"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""

*~*$H!N!NG $T@R*~*

SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

--
...:::Group Rules:::...
http://groups.google.com.pk/group/vu-pink/web/group-rules
^^^^^^
You received this message because you are subscribed to the Google
Groups "vu pink" group.
To post to this group, send email to vu-pink@googlegroups.com
To unsubscribe from this group, send email to
vu-pink+unsubscribe@googlegroups.com
For more options, visit this group at
http://groups.google.com.pk/group/vu-pink?hl=en

--
Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 3/31/2012 05:02:00 AM

--
You received this message because you are subscribed to the Google Groups "VU Computer Science" group.
To post to this group, send email to vu-computer-science@googlegroups.com.
To unsubscribe from this group, send email to vu-computer-science+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/vu-computer-science?hl=en.

Read User's Comments(0)

[Virtual University Of PakistanStudent Cafe] [Pak Youth] ````Daily Quran and Hadith 31 March 2012...````

5:03 AM |

IN THE NAME OF "ALLAH"
Assalamu'alaikum Wa Rahmatullah e Wa Barakatuhu,

Note : If Quran and Hadith is not viewable CLICK HERE to Read Today Quran and Hadith.

Visit
www.quranurdu.com
www.tafheemonline.com
www.quranenglish.com
www.daroos.com
www.islamicrishta.com

"""Don't worry if people hate you because there are many others who love and care you in the earth. But be worried if ALLAH hates you because there is no other who loves and cares you in akhirat."""

*~*$H!N!NG $T@R*~*

SUBHANALLAH HI WABIHAMDIHI SUBHANALLAH HIL AZEEM

--
You received this message because you are subscribed to the Google Groups "Pak Youth" group.
To post to this group, send email to pak-youth@googlegroups.com.
To unsubscribe from this group, send email to pak-youth+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/pak-youth?hl=en.

--
Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 3/31/2012 05:02:00 AM

--
You received this message because you are subscribed to the Google Groups "VU Computer Science" group.
To post to this group, send email to vu-computer-science@googlegroups.com.
To unsubscribe from this group, send email to vu-computer-science+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/vu-computer-science?hl=en.

Read User's Comments(0)

Subscribe to: Posts (Atom)