Re: [DIYbio] Re: Prospects of anti-aging research

>Occam's razor:  that which boosts mating also boots evolutionary selection which equates to health and longevity.   (?) Characterization is a big unknown. 

Disagree. 
Mating selects that you are attractive and in good health until the age of 20-30. By that age you have passed on your genes by making children. 

If you die immediately afterwards or live in bad health, evolution does not care for that too much. 
Of course in social animals it brings benefit to the species if you live another 15 years to help your children grow and surviive So after age 40-50 selection stops anyway.


On Sun, Aug 31, 2014 at 9:08 PM, Jonathan Cline <jcline@ieee.org> wrote:
Occam's razor:  that which boosts mating also boots evolutionary selection which equates to health and longevity.   (?) Characterization is a big unknown.


Figure 2: Score of health status from men and women residents of Carhuamayo, Junin at 4100 m in the Peruvian Central Andes. Upper line: population consuming extracts of maca. Bottom line: population not consuming maca.
  http://www.hindawi.com/journals/ecam/2012/193496.fig.002.jpg


Reference:
http://dx.doi.org/10.1155/2012/193496
Ethnobiology and Ethnopharmacology of Lepidium meyenii (Maca), a Plant from the Peruvian Highlands (2011)



## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
########################

On 8/31/14 1:01 AM, Cathal Garvey wrote:
Maca's effects on human sexuality are less simple than "boosting"; it
appears to improve libido, but has no impact on impotence, for example
(given that this is the primary market for Maca, seems unfair). It is
possible it increases testosterone, but a quick look into Maca after J's
post didn't yield much on anti-aging potential.

There's no contradition between testosterone and oestrogen, contrary to
the popular view that they are in opposition they are important hormones
for both genders.

On 29/08/14 18:41, Mega [Andreas Stuermer] wrote:
If Maca works boosting male fertility, it may increase testosterone levels.

http://jap.physiology.org/content/106/1/333
-> estrogen activates telomerase (in endothelial cells).
Fun fact: aspirin does too

Is that a contradiction?


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Re: [DIYbio] Seed embryo resorption possible?

Thanks, Simon - so we'll flush that idea. 

But - has anyone ever tried taking a seed and injecting pollen from a different plant directly into the seed embryo?




On Saturday, August 30, 2014 6:59:41 PM UTC-5, Simon Field wrote:
The resorption you refer to results in a chimera  -- an organism with two (or more) distinct genomes. The genome that ends up producing the gametes is the one that reproduces. They are not a "cross" like you get when you have two parents.

Sometimes you can tell  just by looking at them. Someone should point out to that first author why his picture of the peacock cannot be an example of X chromosome mosaicism (but it could be Z chromosome mosaicism).

You can get the same result in plants by grafting a branch from one plant onto the trunk of another.


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On Sat, Aug 30, 2014 at 4:00 PM, Warren Notes <warre...@gmail.com> wrote:
Has anyone ever attempted to induce resorption in the plant world for purposes of mixing genes?

In "vanishing twin" syndrome in humans, also known as fetal resorption, one of the fetuses in a multi-gestational pregnancy dies and is partially or completely reabsorbed by its twin.

This is very common in humans - it's estimated that one out of eight of us who DO NOT have a twin STARTED OUT as a twin.

What I'm wondering is - could two seed embryos be removed and then re-housed some sort of synthesized seed coat? If reabsorption occurred in 1/8 of the embryos, as it does in human embryos - would the plant resulting from the reabsortion process be a genetic "cross" of the two embryos?

-John

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Re: [DIYbio] Re: Prospects of anti-aging research

Occam's razor: that which boosts mating also boots evolutionary
selection which equates to health and longevity. (?) Characterization
is a big unknown.


Figure 2: Score of health status from men and women residents of
Carhuamayo, Junin at 4100 m in the Peruvian Central Andes. Upper line:
population consuming extracts of maca. Bottom line: population not
consuming maca.
http://www.hindawi.com/journals/ecam/2012/193496.fig.002.jpg


Reference:
http://dx.doi.org/10.1155/2012/193496
Ethnobiology and Ethnopharmacology of Lepidium meyenii (Maca), a Plant
from the Peruvian Highlands (2011)


## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
########################

On 8/31/14 1:01 AM, Cathal Garvey wrote:
> Maca's effects on human sexuality are less simple than "boosting"; it
> appears to improve libido, but has no impact on impotence, for example
> (given that this is the primary market for Maca, seems unfair). It is
> possible it increases testosterone, but a quick look into Maca after J's
> post didn't yield much on anti-aging potential.
>
> There's no contradition between testosterone and oestrogen, contrary to
> the popular view that they are in opposition they are important hormones
> for both genders.
>
> On 29/08/14 18:41, Mega [Andreas Stuermer] wrote:
>> If Maca works boosting male fertility, it may increase testosterone levels.
>>
>> http://jap.physiology.org/content/106/1/333
>> -> estrogen activates telomerase (in endothelial cells).
>> Fun fact: aspirin does too
>>
>> Is that a contradiction?
>>

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Re: [DIYbio] Re: How to get certain biochemical data?

Hill kinetics is used to calculate cooperativity of binding.

What do you mean they are constant? Do you mean you assume they don't change? 

The number of molecules bound depends on the number of molecules present so you need some number. Are you looking for a simplifying case?

These numbers are not equivalent to Vm and n but you can use these numbers to calculate the Vm and n using Michaelis Menten kinetics.

Also, sorry I meant RNA polymerase not ribosome.



On Sat, Aug 30, 2014 at 11:53 PM, zhen zhang <izgzhen@gmail.com> wrote:
Thank you! It is not a homework, since I am just researching into this topic out of curiosity.

Why I use Hill Kinetics: As I know through a book, the Hill equation can be derived from MM equations, or as a special form of MM. In my opinion, the main difference between these two is the how many binding sites are there. In hill kinetics, there might be multiple binding sites. 

So in your answer, concentration of ribosome, two K_d value and transcription rate can be seen as constant in a particular cell. And I thought they are not  equivalent to K_d, Vm and n.

As far as I know, when repressor binds to the promoter, transcription won't happen. But there might be multiple repressors binding to the promoter to take effect.

However, this problem still annoys me a lot.

Thank you :) 


On Sunday, August 31, 2014 8:31:38 AM UTC+8, Josiah Zayner wrote:
Is there a reason you are using Hill Kinetics?

The max transcription rate should be related to the number of ribosomes bound. Which is related to the number of repressors proteins bound.

Things you need to answer this question:
Concentration of Ribosomes
Concentration of DNA
Concentration of Repressor Protein
Kd of Ribosome for DNA
Kd of Repressor for DNA
Transcription Rate of Single Ribosome

You should be able to use Michaelis Menten Kinetics to solve then


Is this a homework question?

On Friday, August 22, 2014 5:55:03 AM UTC-7, zhen zhang wrote:
For example, I use λ pl promoter to start the downstream transcription, and I use cI as the upstream signal, or a repressor protein, which would inhibit the transcription of pl promoter.

So I suppose that I should use Hill Kinetics to model this regulation process.

So I need three parameter to describe the regulated transcription rate

1. Hill coefficient or the cooperativity constant n.
In the Wikipedia, it give two conditions with n = 2 and n = 3.
2. Dissociation constant Kd which I don't know
3. The Maximum Transcriptional Rate Vm which I also have no idea of.

Where should I go to find the later two parameters on web?
Is there any database that you always use for such condition?

I am not a professional biology researcher, so I have searched so much but ended up with nothing. Thank you a lot if you can help me! 

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Re: [DIYbio] No hay grupo

Google Translation:
"
Hi my name I cristhian hernandez , i am a young biologist and chemist
inform you that there is no community in Ecuador I would like to
encourage these communities here but I like to have
do consent.
"

On Fri, Aug 29, 2014 at 9:40 PM, Cristh Hernandez <cristh614@gmail.com> wrote:
> Hola me llamo cristhian hernandez , soy un joven quimico biologo y
> comunico que no hay comunidad en ecuador me gustaria incentivar estas
> comunidades aqui pero me gustaria tener
> do consentimiento.
>
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Re: [DIYbio] Re: Prospects of anti-aging research

Ah right, after testosterone therapy bodybuilders take beta-blockers, else the testosterone would be converted into estrogen and that would be bad for gaining muscles. Or wold the body just produce less testosterone and estrogen would prevail? Never read too deep into that.


On Sun, Aug 31, 2014 at 10:01 AM, Cathal Garvey <cathalgarvey@cathalgarvey.me> wrote:
Maca's effects on human sexuality are less simple than "boosting"; it
appears to improve libido, but has no impact on impotence, for example
(given that this is the primary market for Maca, seems unfair). It is
possible it increases testosterone, but a quick look into Maca after J's
post didn't yield much on anti-aging potential.

There's no contradition between testosterone and oestrogen, contrary to
the popular view that they are in opposition they are important hormones
for both genders.

On 29/08/14 18:41, Mega [Andreas Stuermer] wrote:
> If Maca works boosting male fertility, it may increase testosterone levels.
>
> http://jap.physiology.org/content/106/1/333
> -> estrogen activates telomerase (in endothelial cells).
> Fun fact: aspirin does too
>
> Is that a contradiction?
>

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Phone: +353876363185
Blog: http://indiebiotech.com
miniLock.io: JjmYYngs7akLZUjkvFkuYdsZ3PyPHSZRBKNm6qTYKZfAM



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Re: [DIYbio] Re: Prospects of anti-aging research

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Maca's effects on human sexuality are less simple than "boosting"; it
appears to improve libido, but has no impact on impotence, for example
(given that this is the primary market for Maca, seems unfair). It is
possible it increases testosterone, but a quick look into Maca after J's
post didn't yield much on anti-aging potential.

There's no contradition between testosterone and oestrogen, contrary to
the popular view that they are in opposition they are important hormones
for both genders.

On 29/08/14 18:41, Mega [Andreas Stuermer] wrote:
> If Maca works boosting male fertility, it may increase testosterone levels.
>
> http://jap.physiology.org/content/106/1/333
> -> estrogen activates telomerase (in endothelial cells).
> Fun fact: aspirin does too
>
> Is that a contradiction?
>

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[DIYbio] Re: How to get certain biochemical data?

Thank you! It is not a homework, since I am just researching into this topic out of curiosity.

Why I use Hill Kinetics: As I know through a book, the Hill equation can be derived from MM equations, or as a special form of MM. In my opinion, the main difference between these two is the how many binding sites are there. In hill kinetics, there might be multiple binding sites. 

So in your answer, concentration of ribosome, two K_d value and transcription rate can be seen as constant in a particular cell. And I thought they are not  equivalent to K_d, Vm and n.

As far as I know, when repressor binds to the promoter, transcription won't happen. But there might be multiple repressors binding to the promoter to take effect.

However, this problem still annoys me a lot.

Thank you :) 


On Sunday, August 31, 2014 8:31:38 AM UTC+8, Josiah Zayner wrote:
Is there a reason you are using Hill Kinetics?

The max transcription rate should be related to the number of ribosomes bound. Which is related to the number of repressors proteins bound.

Things you need to answer this question:
Concentration of Ribosomes
Concentration of DNA
Concentration of Repressor Protein
Kd of Ribosome for DNA
Kd of Repressor for DNA
Transcription Rate of Single Ribosome

You should be able to use Michaelis Menten Kinetics to solve then


Is this a homework question?

On Friday, August 22, 2014 5:55:03 AM UTC-7, zhen zhang wrote:
For example, I use λ pl promoter to start the downstream transcription, and I use cI as the upstream signal, or a repressor protein, which would inhibit the transcription of pl promoter.

So I suppose that I should use Hill Kinetics to model this regulation process.

So I need three parameter to describe the regulated transcription rate

1. Hill coefficient or the cooperativity constant n.
In the Wikipedia, it give two conditions with n = 2 and n = 3.
2. Dissociation constant Kd which I don't know
3. The Maximum Transcriptional Rate Vm which I also have no idea of.

Where should I go to find the later two parameters on web?
Is there any database that you always use for such condition?

I am not a professional biology researcher, so I have searched so much but ended up with nothing. Thank you a lot if you can help me! 

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Re: [DIYbio] Looking for some tobacco BY-2 seeds or callus cells for BioPrinter project

Wonderful - thank you so much!

Patrik

On Saturday, August 30, 2014 11:20:45 PM UTC-7, cory....@gmail.com wrote:
I put the seeds in the mail today.  Not sure what the pickup schedule
is this weekend since it's a federal holiday.  Anyways, it should show
up sometime this week.

-cory

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Re: [DIYbio] Looking for some tobacco BY-2 seeds or callus cells for BioPrinter project

I put the seeds in the mail today. Not sure what the pickup schedule
is this weekend since it's a federal holiday. Anyways, it should show
up sometime this week.

-cory

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[DIYbio] Re: How to get certain biochemical data?

Is there a reason you are using Hill Kinetics?

The max transcription rate should be related to the number of ribosomes bound. Which is related to the number of repressors proteins bound.

Things you need to answer this question:
Concentration of Ribosomes
Concentration of DNA
Concentration of Repressor Protein
Kd of Ribosome for DNA
Kd of Repressor for DNA
Transcription Rate of Single Ribosome

You should be able to use Michaelis Menten Kinetics to solve then


Is this a homework question?

On Friday, August 22, 2014 5:55:03 AM UTC-7, zhen zhang wrote:
For example, I use λ pl promoter to start the downstream transcription, and I use cI as the upstream signal, or a repressor protein, which would inhibit the transcription of pl promoter.

So I suppose that I should use Hill Kinetics to model this regulation process.

So I need three parameter to describe the regulated transcription rate

1. Hill coefficient or the cooperativity constant n.
In the Wikipedia, it give two conditions with n = 2 and n = 3.
2. Dissociation constant Kd which I don't know
3. The Maximum Transcriptional Rate Vm which I also have no idea of.

Where should I go to find the later two parameters on web?
Is there any database that you always use for such condition?

I am not a professional biology researcher, so I have searched so much but ended up with nothing. Thank you a lot if you can help me! 

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Re: [DIYbio] Seed embryo resorption possible?

The resorption you refer to results in a chimera  -- an organism with two (or more) distinct genomes. The genome that ends up producing the gametes is the one that reproduces. They are not a "cross" like you get when you have two parents.

Sometimes you can tell  just by looking at them. Someone should point out to that first author why his picture of the peacock cannot be an example of X chromosome mosaicism (but it could be Z chromosome mosaicism).

You can get the same result in plants by grafting a branch from one plant onto the trunk of another.


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On Sat, Aug 30, 2014 at 4:00 PM, Warren Notes <warrennotes@gmail.com> wrote:
Has anyone ever attempted to induce resorption in the plant world for purposes of mixing genes?

In "vanishing twin" syndrome in humans, also known as fetal resorption, one of the fetuses in a multi-gestational pregnancy dies and is partially or completely reabsorbed by its twin.

This is very common in humans - it's estimated that one out of eight of us who DO NOT have a twin STARTED OUT as a twin.

What I'm wondering is - could two seed embryos be removed and then re-housed some sort of synthesized seed coat? If reabsorption occurred in 1/8 of the embryos, as it does in human embryos - would the plant resulting from the reabsortion process be a genetic "cross" of the two embryos?

-John

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[DIYbio] Seed embryo resorption possible?

Has anyone ever attempted to induce resorption in the plant world for purposes of mixing genes?

In "vanishing twin" syndrome in humans, also known as fetal resorption, one of the fetuses in a multi-gestational pregnancy dies and is partially or completely reabsorbed by its twin.

This is very common in humans - it's estimated that one out of eight of us who DO NOT have a twin STARTED OUT as a twin.

What I'm wondering is - could two seed embryos be removed and then re-housed some sort of synthesized seed coat? If reabsorption occurred in 1/8 of the embryos, as it does in human embryos - would the plant resulting from the reabsortion process be a genetic "cross" of the two embryos?

-John

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Re: {▒**CYBARIEN NETWORK**▒} Notepad secretes

Embedded image permalink


On Sat, Sep 21, 2013 at 5:26 PM, YASIR AWAN <yasirawan1992@gmail.com> wrote:

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Join us for study material like assignments, quiz, gdbs and all that.
 
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by using demooo as id and demooo as password.
Use it Only for fair Purposes. Thanks.
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[**Virtual University Of Pakistan**Student Cafe] .::VULMSIT::.eNoxel.com What does SAP Consultants do on projects ?



 Their role, challenges faced and what they need to achieve as a successful consultant - attend a webinar by our project manager
 
Join us every Monday at 9.30 PM EST. Please find the meeting details at 



This conference seminar will give you a good understanding on what roles you will be playing as an SAP consultant in real projects. 

Thanks

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Posted By Sheroo to **Virtual University Of Pakistan**Student Cafe at 8/30/2014 02:44:00 AM

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[DIYbio] Re: How should I get strarted?

LETS HAVE A RESEARCH ON THE STEM CELLS AS I HAVE HEARD ABOUT THE VARIOUS DRAWBACKS OF IT SUCH AS HEPATOTOXICITY BEING THE ONE SO HOW CAN IT BE REPLACED??


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[DIYbio] Re: How should I get strarted?

I AM NEW IN THIS GROUP BUT I HAD A QUESTION REGARDING BIOCHEMISTRY THAT WHY WAS OR WHAT DRAWBACKS DID THE OLDER SYSTEM OF NOMENCLATURE OF ENZYMES HAD THAT THEY HAD TO REPLACE IT???

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[DIYbio] Re: Can't find supplier for ~1 inch Viton stopper

I have recently used butyl rubber for sealing, it is very easy to work with & clean up easily (even vs. silicone), stays soft & rubbery indefinitely, stays tacky, and very high stretch.  Form it around the wire and neoprene sheath and tighten down washers on both sides, it will compress to form a gasket-seal (ooze out with mechanical compression).   It is also incredibly inexpensive.  There are different product qualities (purities?) so look into the source.  Cheaper versions are sold for automobile doors, HVAC seals, etc.  It will outgas (maybe forever?).  I think it could work well for you.  You'll need maybe a 3" length of 1/4" strip, to hand form into the shape you need, and the lengths you find to buy (comes in rolls) might be 10' etc (a few bucks a foot?), so you'll have enough to last a lifetime.


## Jonathan Cline
## jcline@ieee.org
## Mobile: +1-805-617-0223
########################



On Friday, August 29, 2014 1:30:13 AM UTC-7, Nathan McCorkle wrote:
looking to make a vacuum container wire feed-through, thinking I'll
drill a hole into an existing aluminum blocking plate (maybe with a
chamfer/angle to it), shove some piano wire through the stopper, then
shove that into the blocking plate and pump down. Maybe I'd extend the
existing blocking-plate screws and screw down a clamp on the stopper.
 

 

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[DIYbio] No hay grupo

Hola me llamo cristhian hernandez , soy un joven quimico biologo   y comunico que no hay comunidad en ecuador me gustaria incentivar estas comunidades aqui pero me gustaria tener
do consentimiento.

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[DIYbio] Re: [dorkbotpdx-blabber] Can't find supplier for ~1 inch Viton stopper

On Fri, Aug 29, 2014 at 1:18 PM, Dawes, Andrew M. <dawes@pacificu.edu> wrote:
> The o rings have probably been cleaned and baked, a stopper could have
> enough residual material inside it (like a sponge holding water) that it
> would outgas so much that it looks like a leak -- i.e. virtual leak. You
> could find some vacuum-friendly epoxy (vacseal) and fill a narrow hole in a
> flange but everyone I know who tried to roll their own UHV parts gave up or
> regretted it. It's so picky that there is a reason stuff is expensive.
> Surplus is a great option as long as you can get the parts super clean.
>

Hmm, makes sense. I've seen that epoxy somewhere, though the FIB
operator had a bad experience with epoxy fouling his system (probably
didn't use the 100% solids epoxy, and maybe didn't degas before it
set)... so I'd have to sneak the epoxy in if I wanted (I was thinking
of potting the electronics, if needed).

Thanks for the opinions, they're definitely valuable!

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[DIYbio] Re: [dorkbotpdx-blabber] Can't find supplier for ~1 inch Viton stopper

On Fri, Aug 29, 2014 at 1:05 PM, Jerry Biehler <jerry.biehler@gmail.com> wrote:
> It will leak. I guarantee it. If not you will have a virtual leak. Vacuum
> design is not easy, especially at this level.

Hmm, alright, I have been trying to be careful! Glad I haven't cut
into the blocking-plate yet!

> I have a couple small 3 pin feedthroughs you can have. What kind of voltage
> are you working with? Current?

I think 30V max, not sure of current but the first-application-attempt
is a photodiode in reverse-bias mode, buffered by a MOSFET. These
parts:
BPW34
http://www.jameco.com/webapp/wcs/stores/servlet/Product_10001_10001_1621132_-1
2N7000

http://www.jameco.com/webapp/wcs/stores/servlet/Product_10001_10001_178669_-1

and the schematic (I'm planning on only having the 'light shield'
section in the vacuum, maybe only the photodiode, 10M, and
2N7000)(don't mind the BPW34 being mislabelled on the image):
http://imgur.com/ozKQkUj

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Re: [dorkbotpdx-blabber] [DIYbio] Can't find supplier for ~1 inch Viton stopper

On Fri, Aug 29, 2014 at 12:48 PM, Jerry Biehler <jerry.biehler@gmail.com> wrote:
> If you are trying to get to 10^-8 right now forget the stopper. It is never going to happen with a seal like that. How many connections do you need? What kind of pump are you using to get that low? What kind of chamber?

Oh, why not? I mean, the seal on the blocking plate is just a Viton o-ring...

The setup is an FEI 200 xP, it's got a roughing pump, a turbo, and a ion trap.

I need at least 3 wires to begin, I might be able to get away with 2
if I use the feed-through metal for the ground.

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Re: [dorkbotpdx-blabber] [DIYbio] Can't find supplier for ~1 inch Viton stopper

On Fri, Aug 29, 2014 at 9:06 AM, Jerry Biehler <jerry.biehler@gmail.com> wrote:
> Ahh, I see why viton now. Viton and nitrile are pretty interchangeable for
> vacuum systems. They both have their advantages and disadvantages. Viton is
> more chemical resistant but it also holds water and out gasses longer than
> nitrile. Almost all of the electrometric seals in my system are nitrile and
> I can get down to 10^-8 torr without much problem.

Cool, good to know, I guess I want a nitrile stopper then! That is
something I didn't search for at all... I wonder what NASA has to say
about them (though of course they're probably swinging the temp in
their walk-in space simulator chambers since space is cold).


> How low are you trying to go?

10^-8 is where the instrument hovers I believe.

>
> This is my little system at home: http://flic.kr/p/kaT8XR

Sweet! For imaging?

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Re: [DIYbio] Can't find supplier for ~1 inch Viton stopper

On Fri, Aug 29, 2014 at 10:31 AM, John Griessen <john@industromatic.com> wrote:
> On 08/29/2014 10:36 AM, Nathan McCorkle wrote:
>>
>> I'd like to try and keep the chemical list down to a minimum, as
>> certain materials like to off-gas for a very long time and that
>> behavior can foul the vacuum and imaging system contained therein.
>
>
>
> OK, now I get that you're working on getting real vacuum over time
> and are investigating low cost rubber and wire, so that's why the piano wire
> idea. Piano wire rusts though...
>
> Probably you could get decent results with a sharp sewing needle threaded
> with some
> stainless wire. A #16 or #18 needle could be just right -- they come with
> standard sharp
> points, rounded points triangle knife points for leather. Standard is going
> to be good for your
> test, and qty ten available on ebay for $4 shipped. Maybe even hone it
> some on a fine ceramic whetstone, or piece of alumina like an
> old X86 computer chip package...those light purple ceramic computer chip
> packages.


All good ideas! I'm not sure how much I'd need to worry about rust, as
the inside of the vacuum should be quite dry, and the outside would be
very short... short enough that I could tin the wire with solder.

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[DIYbio] Re: Prospects of anti-aging research

If Maca works boosting male fertility, it may increase testosterone levels. 

http://jap.physiology.org/content/106/1/333 
-> estrogen activates telomerase (in endothelial cells). 
Fun fact: aspirin does too

Is that a contradiction? 

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Re: [DIYbio] Can't find supplier for ~1 inch Viton stopper

On 08/29/2014 10:36 AM, Nathan McCorkle wrote:
> I'd like to try and keep the chemical list down to a minimum, as
> certain materials like to off-gas for a very long time and that
> behavior can foul the vacuum and imaging system contained therein.


OK, now I get that you're working on getting real vacuum over time
and are investigating low cost rubber and wire, so that's why the piano wire
idea. Piano wire rusts though...

Probably you could get decent results with a sharp sewing needle threaded with some
stainless wire. A #16 or #18 needle could be just right -- they come with standard sharp
points, rounded points triangle knife points for leather. Standard is going to be good for your
test, and qty ten available on ebay for $4 shipped. Maybe even hone it
some on a fine ceramic whetstone, or piece of alumina like an
old X86 computer chip package...those light purple ceramic computer chip packages.

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