hello :)
hmm.. the method described initially seems to work still, as far as I know.
@swiftscientist, I'll send you an e-mail :)
On 09/30/2014 07:52 PM, A Scientist wrote:
> Hello code elusive,
>
> Thanks for your great instructions! I followed then to get access to
> this video:
> www.jove.com/video/52017/creating-anatomically-accurate-reproducible-intracranial-xenografts
>
> It gave me this file:
> http://ecsource.jove.com/CDNSource/protected/52017_Keating_081514_P_Web.mp4
>
> But this link does not open. :(
>
> I believe it is something to do with the appearance of the 'protected'
> command in the page address - does this mean we can't download videos
> from jove anymore?
>
> Thank you!
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Re: [DIYbio] Re: Downloading JoVE videos?
Read User's Comments(0)
Re: [DIYbio] Re: Fish gDNA isolation
I am, if only for literature sake.
-- I'd like to bar-code environmental DNA (eDNA) in a local pond but I hear that qPCR is better at it than regular one. Presently, i don't have access to that equipment or know-how.
On Tuesday, September 30, 2014 9:30:35 PM UTC-4, Sebastian wrote:
On Tuesday, September 30, 2014 9:30:35 PM UTC-4, Sebastian wrote:
Perfect! Thanks! I'll post results of the workshop to the list if it works well...in case anyone is interested.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Yuriy Fazylov
Sent: 9/30/2014 9:26 PM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] Re: Fish gDNA isolationhttp://primerdigital.com/dna.--html (bottom of the site)
On Tuesday, September 30, 2014 8:52:39 PM UTC-4, Sebastian wrote:You learn something new everyday! I didn't know tilapia was bred like that. Thanks for the factoid. I was leaning more towards tuna and salmon since Atlantic and pacific have varied prices.
Does anyone think that Edwards Buffer (Tris, EDTA, SDS, NaCl) plus proteinase K would work as a digest buffer? I use it all the time for plant gdna extraction (minus Prot K) and I just grind in buffer, spin down debris, mix supernatant with cold isoprop, and spin for 10mins. Never had a bad pcr using that method so I'm curious if the buffer is versatile enough to use in animal tissue. I know proteinase K is very robust across detergents, pH, etc. Any thoughts?
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Yuriy Fazylov
Sent: 9/30/2014 8:28 PM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] Re: Fish gDNA isolation--Cool. I didn't expect the link to be spot on.If it is a common food stock like Tilapia. Know that some of those are not true species but hybrid of two species. I think it is done to make them hardier and bigger.
On Tuesday, September 30, 2014 1:11:05 PM UTC-4, Sebastian wrote:Thanks for the link. No, its not for glowfish gfp. It's for a workshop Im going to run and would rather not blow through my tissue gdna kit if its not needed. We are barcoding fish found in markets across the NYC and matching the claimed species with the COI gene variation as a fingerprint. Basically showing how pcr can help spot fish fraud. Classic lab exercise. Nothing too exciting.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&DTry Sambrook et al (1989) I haven't read into it personally.
From: Yuriy Fazylov
Sent: 9/30/2014 9:54 AM
To: diy...@googlegroups.com
Subject: RE: [DIYbio] Re: Fish gDNA isolation
This study modified it for fish DNA fingerprinting http://www.nature.com/hdy/journal/v76/n4/abs/hdy199649a. html
Trying to extract GFP from glow fish?
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RE: [DIYbio] Re: Fish gDNA isolation
Perfect! Thanks! I'll post results of the workshop to the list if it works well...in case anyone is interested.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Yuriy Fazylov
Sent: 9/30/2014 9:26 PM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Re: Fish gDNA isolation
http://primerdigital.com/dna.html (bottom of the site)
On Tuesday, September 30, 2014 8:52:39 PM UTC-4, Sebastian wrote:
-- On Tuesday, September 30, 2014 8:52:39 PM UTC-4, Sebastian wrote:
You learn something new everyday! I didn't know tilapia was bred like that. Thanks for the factoid. I was leaning more towards tuna and salmon since Atlantic and pacific have varied prices.
Does anyone think that Edwards Buffer (Tris, EDTA, SDS, NaCl) plus proteinase K would work as a digest buffer? I use it all the time for plant gdna extraction (minus Prot K) and I just grind in buffer, spin down debris, mix supernatant with cold isoprop, and spin for 10mins. Never had a bad pcr using that method so I'm curious if the buffer is versatile enough to use in animal tissue. I know proteinase K is very robust across detergents, pH, etc. Any thoughts?
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Yuriy Fazylov
Sent: 9/30/2014 8:28 PM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] Re: Fish gDNA isolation--Cool. I didn't expect the link to be spot on.If it is a common food stock like Tilapia. Know that some of those are not true species but hybrid of two species. I think it is done to make them hardier and bigger.
On Tuesday, September 30, 2014 1:11:05 PM UTC-4, Sebastian wrote:Thanks for the link. No, its not for glowfish gfp. It's for a workshop Im going to run and would rather not blow through my tissue gdna kit if its not needed. We are barcoding fish found in markets across the NYC and matching the claimed species with the COI gene variation as a fingerprint. Basically showing how pcr can help spot fish fraud. Classic lab exercise. Nothing too exciting.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&DTry Sambrook et al (1989) I haven't read into it personally.
From: Yuriy Fazylov
Sent: 9/30/2014 9:54 AM
To: diy...@googlegroups.com
Subject: RE: [DIYbio] Re: Fish gDNA isolation
This study modified it for fish DNA fingerprinting http://www.nature.com/hdy/journal/v76/n4/abs/hdy199649a. html
Trying to extract GFP from glow fish?
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Re: [DIYbio] Re: Fish gDNA isolation
http://primerdigital.com/dna.html (bottom of the site)
On Tuesday, September 30, 2014 8:52:39 PM UTC-4, Sebastian wrote:
-- On Tuesday, September 30, 2014 8:52:39 PM UTC-4, Sebastian wrote:
You learn something new everyday! I didn't know tilapia was bred like that. Thanks for the factoid. I was leaning more towards tuna and salmon since Atlantic and pacific have varied prices.
Does anyone think that Edwards Buffer (Tris, EDTA, SDS, NaCl) plus proteinase K would work as a digest buffer? I use it all the time for plant gdna extraction (minus Prot K) and I just grind in buffer, spin down debris, mix supernatant with cold isoprop, and spin for 10mins. Never had a bad pcr using that method so I'm curious if the buffer is versatile enough to use in animal tissue. I know proteinase K is very robust across detergents, pH, etc. Any thoughts?
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Yuriy Fazylov
Sent: 9/30/2014 8:28 PM
To: diy...@googlegroups.com
Subject: Re: [DIYbio] Re: Fish gDNA isolation--Cool. I didn't expect the link to be spot on.If it is a common food stock like Tilapia. Know that some of those are not true species but hybrid of two species. I think it is done to make them hardier and bigger.
On Tuesday, September 30, 2014 1:11:05 PM UTC-4, Sebastian wrote:Thanks for the link. No, its not for glowfish gfp. It's for a workshop Im going to run and would rather not blow through my tissue gdna kit if its not needed. We are barcoding fish found in markets across the NYC and matching the claimed species with the COI gene variation as a fingerprint. Basically showing how pcr can help spot fish fraud. Classic lab exercise. Nothing too exciting.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&DTry Sambrook et al (1989) I haven't read into it personally.
From: Yuriy Fazylov
Sent: 9/30/2014 9:54 AM
To: diy...@googlegroups.com
Subject: RE: [DIYbio] Re: Fish gDNA isolation
This study modified it for fish DNA fingerprinting http://www.nature.com/hdy/journal/v76/n4/abs/hdy199649a. html
Trying to extract GFP from glow fish?
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RE: [DIYbio] Re: Fish gDNA isolation
You learn something new everyday! I didn't know tilapia was bred like that. Thanks for the factoid. I was leaning more towards tuna and salmon since Atlantic and pacific have varied prices.
Does anyone think that Edwards Buffer (Tris, EDTA, SDS, NaCl) plus proteinase K would work as a digest buffer? I use it all the time for plant gdna extraction (minus Prot K) and I just grind in buffer, spin down debris, mix supernatant with cold isoprop, and spin for 10mins. Never had a bad pcr using that method so I'm curious if the buffer is versatile enough to use in animal tissue. I know proteinase K is very robust across detergents, pH, etc. Any thoughts?
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
Does anyone think that Edwards Buffer (Tris, EDTA, SDS, NaCl) plus proteinase K would work as a digest buffer? I use it all the time for plant gdna extraction (minus Prot K) and I just grind in buffer, spin down debris, mix supernatant with cold isoprop, and spin for 10mins. Never had a bad pcr using that method so I'm curious if the buffer is versatile enough to use in animal tissue. I know proteinase K is very robust across detergents, pH, etc. Any thoughts?
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Yuriy Fazylov
Sent: 9/30/2014 8:28 PM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Re: Fish gDNA isolation
Cool. I didn't expect the link to be spot on.
If it is a common food stock like Tilapia. Know that some of those are not true species but hybrid of two species. I think it is done to make them hardier and bigger.
On Tuesday, September 30, 2014 1:11:05 PM UTC-4, Sebastian wrote:
Thanks for the link. No, its not for glowfish gfp. It's for a workshop Im going to run and would rather not blow through my tissue gdna kit if its not needed. We are barcoding fish found in markets across the NYC and matching the claimed species with the COI gene variation as a fingerprint. Basically showing how pcr can help spot fish fraud. Classic lab exercise. Nothing too exciting.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&DTry Sambrook et al (1989) I haven't read into it personally.
From: Yuriy Fazylov
Sent: 9/30/2014 9:54 AM
To: diy...@googlegroups.com
Subject: RE: [DIYbio] Re: Fish gDNA isolation
This study modified it for fish DNA fingerprinting http://www.nature.com/hdy/journal/v76/n4/abs/hdy199649a. html
Trying to extract GFP from glow fish?
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[DIYbio] What a 'species' means today... was: Fish gDNA isolation
On Tue, Sep 30, 2014 at 5:28 PM, Yuriy Fazylov <yuriyology@gmail.com> wrote:
> Cool. I didn't expect the link to be spot on.
>
> If it is a common food stock like Tilapia. Know that some of those are not
> true species but hybrid of two species.
In today's world, what should we really think of as a species? If we
were looking at the three animals DNA sequences, the hybrid would just
be the selective merging of the two parent animals. I think it would
be analogous to computer science version control systems... where they
call things branches (I think). Just like code, certain merge
operations end up failing (immediate or latent disease), but sometimes
things are just fine.
So what's interesting about each person essentially being their own
code-branch? Do we say we're all different species, if not, is there
some hard threshold (% difference of parents, or any other sequence
for that matter), or is the threshold more abstract (but still able to
be articulated)?
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Re: [DIYbio] Re: Fish gDNA isolation
Cool. I didn't expect the link to be spot on.
If it is a common food stock like Tilapia. Know that some of those are not true species but hybrid of two species. I think it is done to make them hardier and bigger.
On Tuesday, September 30, 2014 1:11:05 PM UTC-4, Sebastian wrote:
Thanks for the link. No, its not for glowfish gfp. It's for a workshop Im going to run and would rather not blow through my tissue gdna kit if its not needed. We are barcoding fish found in markets across the NYC and matching the claimed species with the COI gene variation as a fingerprint. Basically showing how pcr can help spot fish fraud. Classic lab exercise. Nothing too exciting.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&DTry Sambrook et al (1989) I haven't read into it personally.
From: Yuriy Fazylov
Sent: 9/30/2014 9:54 AM
To: diy...@googlegroups.com
Subject: RE: [DIYbio] Re: Fish gDNA isolation
This study modified it for fish DNA fingerprinting http://www.nature.com/hdy/journal/v76/n4/abs/hdy199649a. html
Trying to extract GFP from glow fish?
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Re: [DIYbio] Re: Fish gDNA isolation
On Tue, Sep 30, 2014 at 3:50 PM, Cathal (Phone) <cathalgarvey@cathalgarvey.me> wrote:
Would love to see the protocols in that paper but..paywall. :(
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RE: [DIYbio] Re: Fish gDNA isolation
Would love to see the protocols in that paper but..paywall. :(
On 30 September 2014 18:11:01 GMT+01:00, scocioba@gmail.com wrote:
Thanks for the link. No, its not for glowfish gfp. It's for a workshop Im going to run and would rather not blow through my tissue gdna kit if its not needed. We are barcoding fish found in markets across the NYC and matching the claimed species with the COI gene variation as a fingerprint. Basically showing how pcr can help spot fish fraud. Classic lab exercise. Nothing too exciting.
Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&DTry Sambrook et al (1989) I haven't read into it personally.
From: Yuriy Fazylov
Sent: 9/30/2014 9:54 AM
To: diybio@googlegroups.com
Subject: RE: [DIYbio] Re: Fish gDNA isolation
This study modified it for fish DNA fingerprinting http://www.nature.com/hdy/journal/v76/n4/abs/hdy199649a.html
Trying to extract GFP from glow fish?
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Sr. SSAS Developer to work out of Long Island NY - Phone hire.
They need a SSAS based developer who is very good with SSAS and can build cubes.
Sr. SSAS Developer
Long Island, NY
Hiring will be done over the phone
Client need a very experienced Sr. SSAS Developer – not a person who can simply do day to day work but someone who is self-directed.
Someone who can figure out be could troubleshoot our current setup and guide us on how best to address the issues.
Thanks,
Dhruv Soni
Phone : 218-656-0396
Email : Dhruv@riderconsultinginc.com
Gtalk : rider.dhruv1
[DIYbio] Oxalobacter formigenes probiotic suppliers? was: (Re)Becoming Human: what happened the day I replaced 99% of the genes in my body
The article mentions Oxalobacter formigenes being present in significant percentage in the Hadza, and that this is something that western microbiomes pretty much lack.
-- I tried googling oxalobacter probiotic, but didn't get very far. Is this something that doesn't exist? If it does, can someone post a link here? I've got a friend with kidney stones who has been living with them due to lack of western healthcare insurance.
The role of Oxalobacter formigenes colonization in calcium oxalate stone disease.
On Tue, Sep 30, 2014 at 1:21 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
The real link for anyone interested:
http://humanfoodproject.com/rebecoming-human-happened-day-replaced-99-genes-body-hunter-gatherer/
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Lead Informatica- JOB OPPORTUINITY
Hello,
Hope you are doing well,
Please go through the following requirement and let me know on Dhruv@riderconsultinginc.com if you have any available
Role Infromatica Lead
Location NH
Duration Long Term
SKILL-
•ILM Test Data Management 9.1 & 9.5:
•Trained in ILM TDM and Implemented Informatica Data Subset and Data Masking project from off-shore.
•Experience in configuring Informatica Services and ILM Test Data Management software.
•Involved in Masking integrated Applications using complex masking techniques.
•Involved in gathering the requirements, Analysis, preparing the estimates, project and resource loading plan, Statement of work and driving the off-shore team.
•Resolved complex issues came up during the TDM installation and development phase by coordinating with Informatica.
Thanks,
Dhruv Soni
Phone : 218-656-0396
Email : Dhruv@riderconsultinginc.com
Gtalk : rider.dhruv1
Very urgent requirement Tibco Developer in NC Immediate interview
Hello,
Hope you are doing well,
Please go through the following requirement and let me know on Dhruv@riderconsultinginc.com if you have any available
Role Tibco Developer
Location: Charlotte, NC
Interview Process: One phone screen. May do two, but not usually
Length: 18 Months
Requirements:
8-10 years experience working with TIBCO suite of products
Strong working knowledge of both TIBCO Business Works and TIBCO Business Events - using the latest versions
Nice to have:
Experience working in environment where they are developing their own infrastructure
Thanks,
Dhruv Soni
Phone : 218-656-0396
Email : Dhruv@riderconsultinginc.com
Gtalk : rider.dhruv1
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