Re: [DIYbio] Re: Free biosafety advice kits for community labs --> Ask a Biosafety Expert poster and magnet

2:42 PM |

Anything available for biolabs in the UK?

On 31 October 2014 19:01, Jason Bobe <jasonbobe@gmail.com> wrote:
Another shipment of biosafety kits went out earlier this week. We've got about a dozen more kits available.  If you want one for your community lab or hackerspace or tech accelerator / incubator, please sign-up here:


DIYbio.org's biosafety advisory is a group of professional biosafety experts with training and experience with common biosafety topics, such as: disinfection, safety equipment and personal protective equipment, waste and disposal, laboratory practices, transport of biological substances, facility design. 

If you working on a project and have a biosafety related questions? Now is your chance to ask a group of volunteer biosafety advisors for free:

Jason 

On Tuesday, June 10, 2014 5:16:26 PM UTC-4, Jason Bobe wrote:
First shipment of biosafety kits went out today, thanks to everyone who signed-up.  

If any other U.S. based community labs / hackerspaces want a kit, please sign-up now, I plan to do another shipment next week:


Jason Bobe

On Thursday, June 5, 2014 6:18:04 PM UTC-4, Jason Bobe wrote:
Hey All -

The Ask a Biosafety Expert (ABE) service by DIYbio.org provides free biosafety advice from a panel of volunteer experts to the DIYbio community. Check it out here: http://ask.diybio.org/


We are offering a free biosafety advice kits for community labs based in the United States. Each kit includes a poster and a magnet featuring awesome artwork by the llustrator Himanshu Sharma (http://www.gohemu.com/), as well as a printed copy of a recent report about the DIYbio community (Here is a PDF of 7 myths & realities).


Here is a pic:


Want a kit? Please follow the link below and give us some info and we'll ship you a kit. 1 kit per community lab. Don't belong to a community lab, but still want a kit? Outside the U.S.? Stay tuned, we plan to make these available to individual practitioners and international folks at a later time.


Sign-up here:


Jason Bobe

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Posted by Science

Re: [DIYbio] miniPCR is live on kickstarter

1:12 PM |

On 10/31/2014 01:56 PM, scocioba@gmail.com wrote:
> I don't want to seem rude but that price point is ridiculous.

Not just int'l shipping but the device itself.

I think miniPCR has an enclosure design that is nice looking,
but with laborious assembly time required.
Enclosures for a specialized thing with air flow paths, light paths, etc
need to be a custom shape which can be made by milling machine in plastic in
low volumes, then the same shape molded via a mid volume injection-mold-tool
in aluminum like protomold sells. Whoever is assembling that clear acrylic
is getting paid, which is driving the sell price up.

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Posted by Science

[DIYbio] Re: Free biosafety advice kits for community labs --> Ask a Biosafety Expert poster and magnet

12:01 PM |

Another shipment of biosafety kits went out earlier this week. We've got about a dozen more kits available.  If you want one for your community lab or hackerspace or tech accelerator / incubator, please sign-up here:


DIYbio.org's biosafety advisory is a group of professional biosafety experts with training and experience with common biosafety topics, such as: disinfection, safety equipment and personal protective equipment, waste and disposal, laboratory practices, transport of biological substances, facility design. 

If you working on a project and have a biosafety related questions? Now is your chance to ask a group of volunteer biosafety advisors for free:

Jason 

On Tuesday, June 10, 2014 5:16:26 PM UTC-4, Jason Bobe wrote:
First shipment of biosafety kits went out today, thanks to everyone who signed-up.  

If any other U.S. based community labs / hackerspaces want a kit, please sign-up now, I plan to do another shipment next week:


Jason Bobe

On Thursday, June 5, 2014 6:18:04 PM UTC-4, Jason Bobe wrote:
Hey All -

The Ask a Biosafety Expert (ABE) service by DIYbio.org provides free biosafety advice from a panel of volunteer experts to the DIYbio community. Check it out here: http://ask.diybio.org/


We are offering a free biosafety advice kits for community labs based in the United States. Each kit includes a poster and a magnet featuring awesome artwork by the llustrator Himanshu Sharma (http://www.gohemu.com/), as well as a printed copy of a recent report about the DIYbio community (Here is a PDF of 7 myths & realities).


Here is a pic:


Want a kit? Please follow the link below and give us some info and we'll ship you a kit. 1 kit per community lab. Don't belong to a community lab, but still want a kit? Outside the U.S.? Stay tuned, we plan to make these available to individual practitioners and international folks at a later time.


Sign-up here:


Jason Bobe

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Posted by Science

RE: [DIYbio] miniPCR is live on kickstarter

11:56 AM |

The obviously have to ship it by paddle-boat. Seems super reasonable if that's the method in question...

I don't want to seem rude but that price point is ridiculous. Not just int'l shipping but the device itself. I STILL have yet to hear of any passable justification for the device being so damn expensive for what it is...or what its not. I wouldn't even be able to do an overnight ligation since it can't get colder that ~22C. I'd pay $250 retail. Maximum.

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Andy Chen
Sent: ‎10/‎31/‎2014 2:42 PM
To: diybio@googlegroups.com
Subject: [DIYbio] miniPCR is live on kickstarter

$200 for international shipping....? Seriously?

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Posted by Science

RE: [DIYbio] Rich Lee, with magnetic earphone Implants, shares details

11:52 AM |

I wonder how he isn't getting any side effects from the metals in the magnet. PTFE coating like surgical implants?

Sebastian S. Cocioba
CEO & Founder
New York Botanics, LLC
Plant Biotech R&D
From: Corey McCarren
Sent: ‎10/‎31/‎2014 2:42 PM
To: diybio@googlegroups.com
Subject: [DIYbio] Rich Lee, with magnetic earphone Implants, shares details

Rich Lee has three magnetic implants in his body -- two in his ears, which he uses as "headphones."

"The implant in my fingertip is surrounded by sensitive nerves. Whenever the magnet passes by an electromagnetic field it moves in response. AC currents are especially noticeable. It almost feels like a texture. Basically it allows you to feel magnetic fields, which is an otherwise invisible world.

The implants in my ears are a bit different. I use a special transmitting coil which is worn around my neck or inside of a hat. That coil is plugged in to the headphone port of a device, such as a mobile phone. Sound from the device is carried through the coil in the form of electricity which creates a magnetic field. This of course causes the magnets in my ears to twitch and actually creates sound that I can hear in my implants. It is really cool. Most normal speakers work on a similar principle, except they usually use a magnet to move a coil, whereas I use the coil to move the magnet."


http://titanovo.com/biohacker-rich-lee-shares-details-earphone-implants/

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Posted by Science

Urgent requirement : SAP BOBJ Data Visualization Sr. Developer/ Architect : Columbus : Immediate interview

11:27 AM |

Hello,                                               

Hope you are doing well,                                                                                                                                          

Please go through the following requirement and let me know on Dhruv@riderconsultinginc.com if you have any available

 

 

Role SAP BOBJ Data Visualization Sr. Developer/ Architect

Location Columbus,

Duration 1 years


Skills:
- BOBJ expertise primarily in Design Studio and Dashboard Design
- Expertise in other BOBJ tools a plus – WebI, Explorer, Lumira, etc.
- Expertise in working with the BOBJ/ Design Studio SDK
- Knowledge/ expertise in integrating content with various portals like SharePoint, SAP Portal, etc.
- Knowledge of integrating BOBJ Content with Universes, BW Queries, etc.
- Self-starter
- Ability to lead and manage customer expectations
- Ability to architect visualization solutions with a focus on usability & user experience
- Good communications skills
- Expertise in other visualizations tools like Tableau, QlikView, etc.
- Expertise in using open source visualization libraries
- Knowledge/ expertise in UI Frameworks, Mobile, etc.?

 

 

Thanks,
Dhruv Soni

Phone : 218-656-0396
Email : Dhruv@riderconsultinginc.com
Gtalk : rider.dhruv1

 

 

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Posted by Science

Re: [DIYbio] Experiments for PCR at home or school education

10:35 AM |

On Fri, Oct 31, 2014 at 4:41 AM, Mike Petersen <mike2b17@googlemail.com> wrote:
> Hi everybody,
>
> I wonder if there are any step by step experiments available on the
> internet, what to do with a PCR and a gel electrophoresis.
> Any school experiments available like for example DNA barcoding and other
> things?

Have you looked here? They have great stuff at very reasonable prices:
http://www.carolina.com/biotechnology-teaching-resources/10101.ct

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Posted by Science

Re: [DIYbio] Experiments for PCR at home or school education

10:08 AM |

Why not try plant barcoding. If you have a pcr machine and gel box, buy some taq master mix off ebay, ship primers to a local business or school from life technologies value oligos, and send extract genomic dna from local plants, run the barcoding primers, extract the amplified dna, and send it to the dna barcoding initiative for sequencing. The do huge discounts for schools IIRC.

Sent from my iPad

On Oct 31, 2014, at 7:41 AM, Mike Petersen <mike2b17@googlemail.com> wrote:

Hi everybody,

I wonder if there are any step by step experiments available on the internet, what to do with a PCR and a gel electrophoresis.
Any school experiments available like for example DNA barcoding and other things?

Thank you :)


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Posted by Science

Fwd: Urgent Client Need: UI Lead Engineer; Sanjose, CA; 2+Years Contract

6:39 AM |

Please send your resumes at niranjan.kumar@vision3solutions.com

 

Hi,

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

Title: UI Lead Engineer

Location: Sanjose, CA
Duration: 2+Years Contract

 

Skills:

·         Front end Expert, HTML5, CSS , PHP,  JavaScript ( JS libraries – jQuery, extJS..etc), RWD frameworks ( 960grid, ,Twitter Bootstrap, Foundation, Skeleton, HTML5 Boilerplate, Montage     HTML 5 framework, LESS framework), Accessibility, IE6 quicks, JSON, Mobile UI,CSS3, CSS Hacks, less CSS, SASS, Media queries, MVC frameworks (AngularJS), Github..etc

·         Good Experience JAVASCRIPT and expert in JQuery library ( jqgrids, JS components) and its USAGE.

·         Strong XHTML,PHP, HTML5 development skills.; knowledge of how to use appropriate hooks in XHTML to limit the amount of classes and ID's required on a page, expert in IE quicks.

·         Deep understanding of CSS inheritance, CSS 3 Media Queries.

·         Usage of Front End Frameworks and templates ( e.x: 360grid, bootstrap, 960GS, Blueprint CSS Grid System, HTML Boilerplate)

·         Expert in Web / Mobile development.

·         The Developer will work with a team of UI developers/designers and collaborate with PM's, Digital Marketing, User Experience, BAs and Online Development teams to build conceptual and functional prototypes and production ready UI code.

·         The potential candidate should have excellent communication skills with an ability to interact with all levels of the organization from Developers to Senior Management.

·         Ability to work in a team environment, Strong working knowledge of Photoshop; able to cutup comps from the design team.

·         Working knowledge of other Java Script libraries, ex:  YUI, EXTJS, .etc

·         CSS frameworks ( less.css etc)

·         Experience designing and coding responsive UI jQuery Mobile Creating/building reusable UI components with jQuery Experience with Apahce/MySQL/PHP Experience with client side templating, such as handlebar.js

·         Familiarity with data visualization frameworks such as D3 JS. Experience with retirement enrollment products (enrollment, manage contribution, etc) preferred but not required.

·         Hands on experience with Adobe suites of products - Photoshop, Dreamweaver, Fireworks, Illustrator Excellent written and verbal communication Likes to innovate and invent, but always with a clear goal.

 

Required Skills:

·         JavaScript with Dojo is a Plus

·         Mobile based applications using jQuery Mobile bootstrap

 

Education

Bachelor's degree in Computer Science or equivalent, or ten or more years of relevant experience

 

 

Thanks& Regards

 

Niranjan Kumar

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com

 

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Posted by Science

Urgent Client Need: UI Lead Engineer; Sanjose, CA; 2+Years Contract

6:38 AM |

Please send your resumes at niranjan.kumar@vision3solutions.com

 

Hi,

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

Title: UI Lead Engineer

Location: Sanjose, CA
Duration: 2+Years Contract

 

Skills:

·         Front end Expert, HTML5, CSS , PHP,  JavaScript ( JS libraries – jQuery, extJS..etc), RWD frameworks ( 960grid, ,Twitter Bootstrap, Foundation, Skeleton, HTML5 Boilerplate, Montage     HTML 5 framework, LESS framework), Accessibility, IE6 quicks, JSON, Mobile UI,CSS3, CSS Hacks, less CSS, SASS, Media queries, MVC frameworks (AngularJS), Github..etc

·         Good Experience JAVASCRIPT and expert in JQuery library ( jqgrids, JS components) and its USAGE.

·         Strong XHTML,PHP, HTML5 development skills.; knowledge of how to use appropriate hooks in XHTML to limit the amount of classes and ID's required on a page, expert in IE quicks.

·         Deep understanding of CSS inheritance, CSS 3 Media Queries.

·         Usage of Front End Frameworks and templates ( e.x: 360grid, bootstrap, 960GS, Blueprint CSS Grid System, HTML Boilerplate)

·         Expert in Web / Mobile development.

·         The Developer will work with a team of UI developers/designers and collaborate with PM's, Digital Marketing, User Experience, BAs and Online Development teams to build conceptual and functional prototypes and production ready UI code.

·         The potential candidate should have excellent communication skills with an ability to interact with all levels of the organization from Developers to Senior Management.

·         Ability to work in a team environment, Strong working knowledge of Photoshop; able to cutup comps from the design team.

·         Working knowledge of other Java Script libraries, ex:  YUI, EXTJS, .etc

·         CSS frameworks ( less.css etc)

·         Experience designing and coding responsive UI jQuery Mobile Creating/building reusable UI components with jQuery Experience with Apahce/MySQL/PHP Experience with client side templating, such as handlebar.js

·         Familiarity with data visualization frameworks such as D3 JS. Experience with retirement enrollment products (enrollment, manage contribution, etc) preferred but not required.

·         Hands on experience with Adobe suites of products - Photoshop, Dreamweaver, Fireworks, Illustrator Excellent written and verbal communication Likes to innovate and invent, but always with a clear goal.

 

Required Skills:

·         JavaScript with Dojo is a Plus

·         Mobile based applications using jQuery Mobile bootstrap

 

Education

Bachelor's degree in Computer Science or equivalent, or ten or more years of relevant experience

 

 

Thanks& Regards

 

Niranjan Kumar

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com

 

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Posted by Science

[DIYbio] Anyone at iGem?

5:52 AM |

Hello

Anyone want to meet up that's at iGem?

-Koeng

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[DIYbio] Experiments for PCR at home or school education

4:41 AM |

Hi everybody,

I wonder if there are any step by step experiments available on the internet, what to do with a PCR and a gel electrophoresis.
Any school experiments available like for example DNA barcoding and other things?

Thank you :)


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Posted by Science

[DIYbio] Re: miniPCR is live on kickstarter

4:31 AM |

I´m interested. I´m from Germany. Where are you from? Anyone else from Europe?

Am Donnerstag, 30. Oktober 2014 18:20:36 UTC+1 schrieb Kermit Henson:
Anyone from Europe who wish to share a bulk? I believe it is a way to reduce shipping costs

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[**Virtual University Of Pakistan**Student Cafe] .::VULMSIT::.eNoxel.com Got Trained in SAP now do you know what you will do on a job as a SAP consultant ?

2:47 AM |



Watch Recorded Video of what exactly a SAP Consultant does on projects. If you are new to SAP and want to learn SAP or attend free Webinars please email training@101erp.com
 


Sign up for live webinar by emailing project@101erp.com




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Virtual University of Pakistan*** IT n CS Blog
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http://www.eNoxel.com
http://www.enoxelit.tk
http://www.geniusweb.tk
 
and Please do Share this group with your Friends and Class Fellows so that our Circle would expand and can be more useful for other Students.
 
Thanks, n Best of Luck......
 
 
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[DIYbio] miniPCR is live on kickstarter

11:35 PM |

$200 for international shipping....? Seriously?

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[DIYbio] Rich Lee, with magnetic earphone Implants, shares details

5:35 PM |

Rich Lee has three magnetic implants in his body -- two in his ears, which he uses as "headphones."

"The implant in my fingertip is surrounded by sensitive nerves. Whenever the magnet passes by an electromagnetic field it moves in response. AC currents are especially noticeable. It almost feels like a texture. Basically it allows you to feel magnetic fields, which is an otherwise invisible world.

The implants in my ears are a bit different. I use a special transmitting coil which is worn around my neck or inside of a hat. That coil is plugged in to the headphone port of a device, such as a mobile phone. Sound from the device is carried through the coil in the form of electricity which creates a magnetic field. This of course causes the magnets in my ears to twitch and actually creates sound that I can hear in my implants. It is really cool. Most normal speakers work on a similar principle, except they usually use a magnet to move a coil, whereas I use the coil to move the magnet."


http://titanovo.com/biohacker-rich-lee-shares-details-earphone-implants/

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Fwd: Urgent Client Need: UI Lead Engineer; Sanjose, CA; 12+Years Contract

2:17 PM |

Please send your resumes at niranjan.kumar@vision3solutions.com

 

Hi,

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

Title: UI Lead Engineer

Location: Sanjose, CA
Duration: 2+Years Contract

 

Skills:

·         Front end Expert, HTML5, CSS , PHP,  JavaScript ( JS libraries – jQuery, extJS..etc), RWD frameworks ( 960grid, ,Twitter Bootstrap, Foundation, Skeleton, HTML5 Boilerplate, Montage     HTML 5 framework, LESS framework), Accessibility, IE6 quicks, JSON, Mobile UI,CSS3, CSS Hacks, less CSS, SASS, Media queries, MVC frameworks (AngularJS), Github..etc

·         Good Experience JAVASCRIPT and expert in JQuery library ( jqgrids, JS components) and its USAGE.

·         Strong XHTML,PHP, HTML5 development skills.; knowledge of how to use appropriate hooks in XHTML to limit the amount of classes and ID's required on a page, expert in IE quicks.

·         Deep understanding of CSS inheritance, CSS 3 Media Queries.

·         Usage of Front End Frameworks and templates ( e.x: 360grid, bootstrap, 960GS, Blueprint CSS Grid System, HTML Boilerplate)

·         Expert in Web / Mobile development.

·         The Developer will work with a team of UI developers/designers and collaborate with PM's, Digital Marketing, User Experience, BAs and Online Development teams to build conceptual and functional prototypes and production ready UI code.

·         The potential candidate should have excellent communication skills with an ability to interact with all levels of the organization from Developers to Senior Management.

·         Ability to work in a team environment, Strong working knowledge of Photoshop; able to cutup comps from the design team.

·         Working knowledge of other Java Script libraries, ex:  YUI, EXTJS, .etc

·         CSS frameworks ( less.css etc)

·         Experience designing and coding responsive UI jQuery Mobile Creating/building reusable UI components with jQuery Experience with Apahce/MySQL/PHP Experience with client side templating, such as handlebar.js

·         Familiarity with data visualization frameworks such as D3 JS. Experience with retirement enrollment products (enrollment, manage contribution, etc) preferred but not required.

·         Hands on experience with Adobe suites of products - Photoshop, Dreamweaver, Fireworks, Illustrator Excellent written and verbal communication Likes to innovate and invent, but always with a clear goal.

 

Required Skills:

·         JavaScript with Dojo is a Plus

·         Mobile based applications using jQuery Mobile bootstrap

 

Education

Bachelor's degree in Computer Science or equivalent, or ten or more years of relevant experience

 

 

Thanks& Regards

 

Niranjan Kumar

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com

 

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Urgent Client Need: UI Lead Engineer; Sanjose, CA; 12+Years Contract

2:13 PM |

Please send your resumes at niranjan.kumar@vision3solutions.com

 

Hi,

 

This is Niranjan from Vision3solutions; hope you are doing well.

Please go through below description and reply with your resume, contact details and current location, if you feel comfortable

 

Title: UI Lead Engineer

Location: Sanjose, CA
Duration: 2+Years Contract

 

Skills:

·         Front end Expert, HTML5, CSS , PHP,  JavaScript ( JS libraries – jQuery, extJS..etc), RWD frameworks ( 960grid, ,Twitter Bootstrap, Foundation, Skeleton, HTML5 Boilerplate, Montage     HTML 5 framework, LESS framework), Accessibility, IE6 quicks, JSON, Mobile UI,CSS3, CSS Hacks, less CSS, SASS, Media queries, MVC frameworks (AngularJS), Github..etc

·         Good Experience JAVASCRIPT and expert in JQuery library ( jqgrids, JS components) and its USAGE.

·         Strong XHTML,PHP, HTML5 development skills.; knowledge of how to use appropriate hooks in XHTML to limit the amount of classes and ID's required on a page, expert in IE quicks.

·         Deep understanding of CSS inheritance, CSS 3 Media Queries.

·         Usage of Front End Frameworks and templates ( e.x: 360grid, bootstrap, 960GS, Blueprint CSS Grid System, HTML Boilerplate)

·         Expert in Web / Mobile development.

·         The Developer will work with a team of UI developers/designers and collaborate with PM's, Digital Marketing, User Experience, BAs and Online Development teams to build conceptual and functional prototypes and production ready UI code.

·         The potential candidate should have excellent communication skills with an ability to interact with all levels of the organization from Developers to Senior Management.

·         Ability to work in a team environment, Strong working knowledge of Photoshop; able to cutup comps from the design team.

·         Working knowledge of other Java Script libraries, ex:  YUI, EXTJS, .etc

·         CSS frameworks ( less.css etc)

·         Experience designing and coding responsive UI jQuery Mobile Creating/building reusable UI components with jQuery Experience with Apahce/MySQL/PHP Experience with client side templating, such as handlebar.js

·         Familiarity with data visualization frameworks such as D3 JS. Experience with retirement enrollment products (enrollment, manage contribution, etc) preferred but not required.

·         Hands on experience with Adobe suites of products - Photoshop, Dreamweaver, Fireworks, Illustrator Excellent written and verbal communication Likes to innovate and invent, but always with a clear goal.

 

Required Skills:

·         JavaScript with Dojo is a Plus

·         Mobile based applications using jQuery Mobile bootstrap

 

Education

Bachelor's degree in Computer Science or equivalent, or ten or more years of relevant experience

 

 

Thanks& Regards

 

Niranjan Kumar

Vision3 Solutions Inc

8155 Cavendish Place,Suwanee, GA 30024
Contact No: (678) 554-4785
Email: niranjan.kumar@vision3solutions.com

                 niruk5503@gmail.com

 

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