Science

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Java Developer+ DevOps / Linux scripting

Sunnyvale, CA

6+ Months

Skype interview

Primary Skill: JAVA

Strong hands-on experience with one or more of: Java 8, Tomcat, Java EE.

Experience with RESTful web services is a must.

Experience with full stack development (Basic Java script/ HMTL / CSS and JQuery) .

Hands-on experience on Oracle database, Writing PL/SQL , Procedures, working on performance tuning

Working exposure to Shell scripting, DevOps concepts, Build Tools like Jenkins, Maven.

Familiarity with scaling applications, integration projects which communicates to multiple components.

Nice to have - Experience on IIS, .NET framework, Windows for (Application Installation, Configuration, Debugging).

Nice to have - Exposure to Linux environments, patching, Experience with infra migration projects.

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Web:  http://www.pyramidci.com/staffing-home

We Find Hidden Talent

I am a Molecular Biology Resarch Assistant based in Edinburgh and I recently came across an interesting paper where they used modelling for coming up with deletion strategies that allow increased production of cis-aconitic acid in E. coli (you can read all about it here: https://www.ncbi.nlm.nih.gov/pubmed/27269589). SInce I found this approach particularly powerful, I decided to do some modelling out of interest. I am planning on using the python package Cobrapy for this (http://opencobra.github.io/cobrapy/) as I don't know how to use MATLAB. After I will have identified some deletion strategies, I could test them directly as I have access to a lab. I was therefore wondering if there is anybody on here who has experience with this package or constraint-based modelling in general and would like to collaborate in this small project. 

There are people who have successfully built gene guns for a few dozens of bucks. Avoids agrobacterium regulation

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Java Developer

Sunnyvale, CA

6+ Months

Skype Interview

·         Please find the job description of the position.

·         Fluent in Java 6, and at least working knowledge of Java 8

·         Strong Object Oriented Design background

·         Comfortable with Spring

·         Experience with Cassandra

·         Basic knowledge of Kafka

·         Understanding of Reactive Functional Programming (preferred)

·         Detail-oriented, self-starter, strong written and oral communication skills

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Web:  http://www.pyramidci.com/staffing-home

We Find Hidden Talent

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Kafka Expert Developer

Sunnyvale, CA

6+ Months

Primary skill: Kafka
Kafka - architecture, stabilization. Must have 2-3 years Kafka experience.

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Web:  http://www.pyramidci.com/staffing-home

We Find Hidden Talent

Thanks for sharing your estimates. I am also unsure about the numbers. 5000 seemed like a decent estimate at first. Some, not all, growers are doing pretty advanced molecular stuff. Also, I remember deriving my first design of a laminar flow hood from a description on a orchid amateur website: http://www.orchideenvermehrung.at/english/lfh/use.htm

Anyhow, I just returned from Warsaw and Bucharest where I met with people active in DIYBio stuff around there. Even though these people never popup on the mail lists it is pretty cool stuff they are up to. I will show the footage of a Warsaw basement lab in the next vlog

On Saturday, 11 March 2017 06:29:27 UTC+1, Marc Dusseiller wrote:

interesting discussion...

from my side, having visited many places that are doings stuff around DIYbio, i would also reduce the numbers a lot.

for now it doesnt make sense to include the foodies and growers, cos your policy work is more specific i think.

if you also not count a random person that once joined a "strawberry DNA extraction" at a booth in a makerfaire...

talking for europe i would but the number even below 500.

looking at who is active in the various maillists... i' even go down to 100.

sorry pieter... why you even want to put a number like 5000 in Europe?

so, globally we can still stand for something like 2000?

greets,

marc

On Saturday, March 11, 2017 at 12:35:24 AM UTC+8, Pieter wrote:

The thing with statistics is of course the problem of scope definition. I'd very interested in setting up a new survey!

@Sebastian, once we start including the home growers in the definition, 5.000 is all of sudden a very conservative estimate. On itself http://www.wietforum.nl has already 32.000 members. Although there is not much molecular stuff going on over there. The bulb flower breeding community is more active on that front.

On Friday, 3 March 2017 06:30:15 UTC+1, Sebastian wrote:

I wonder how many plant people are on the list. Show of hands? 

Sebastian S. Cocioba

CEO & Founder

New York Botanics, LLC

Blog: ATinyGreenCell.com

On Mar 2, 2017, at 11:47 AM, Pieter Waag <pie...@waag.org> wrote:

We just published the third episode of our #OPENBIOTECH vlog series: https://www.youtube.com/watch?v=WpVp9YH8Znc

This time I am trying to do a sensible estimate of the size of the DIYBio community. I really wonder what your best estimate would be. 

There are nearly 6000 people on this mailing list, but are these really all active DIYBiologists? The mailing list of our lab in Amsterdam alone already contains over 1000 people, so I guess that's not a great way to make an estimate.

On the "local" lab page of the diybio.org site there are about 100 labs listed. Does every lab have at least 10, 50 or perhaps 100 active members?

And how about the DIY neuro/brain, (animal) breeders, nutrition/foodhackers, grinders/bodyhackers (just check #biohacking on instagram)? Do you think these should be considered as part of the DIYBio movement as well? The vlog also contains an interview with Christine Marizzi of the DNA Barcoding project. In think it is fair to include their participants as well. Do you agree?

For the vlog I assumed it was safe to say there are about 5,000 people in Europe involved in this scene. That's my best guess for now. 500 feels like a too small number to me, while 50,000 is way too much. So I suppose the order of magnitude is right. 

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For those of us without access to biohazard waste receptacles, how do you safely dispose of old cultures? For example, non-pathogenic E. coli (BL21, DH10b). Is it safe enough to just toss in the trash? Or what is an effective way to ensure sterilization (in non-autoclaveable plates) before heading to the trash?

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Kafka Expert

Sunnyvale, CA

6+ Months

Primary skill: Kafka
Kafka - architecture, stabilization. Must have 2-3 years Kafka experience.

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 415-943-9386 Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Web:  http://www.pyramidci.com/staffing-home

We Find Hidden Talent

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

Tableau Administrator with Strong SQL experience

 San Jose, CA

6+ Months

Hangout Interview

Minimum relevant experience 5+ years of experience in Business Intelligence and Reporting/Visualization tool administration (preferably, Tableau)

Description:

1.            Gather requirements, design, build, test and deploy Tableau reports

2.            Provide hands-on Tableau production support. Identify and research problem trends and work with internal or external subject matter experts to correct and proactively prevent further problems by recommending and/or implementing bug-fixes, application enhancements, customer training, business process changes or other measures

3.            Administer and support a multi-tier Tableau infrastructure (Tableau Server, Server Arch, Server side components) including upgrade analysis and planning, security administration, release management, troubleshooting, performance tuning, and general systems maintenance

4.            Experiment with new features and enhancements released by Tableau, and bring them into the platform & organization.

Technical Skills:

•             Tableau Desktop, building and publishing dashboards

•             Tableau Server

•             SQL skills

•             Data warehousing, data modelling

•             Problem-solving, troubleshooting skills

•             Requirements gathering, documenting design/processes

•             Big Data

Soft Skills:

•             Excellent communication skills: Role involves interfacing with internal customers, external software vendors

•             Organization skills: Ability to keep up with high volume of tickets/requests

•             Self-starter: Role requires proactively initiating tasks. Ex: planning for upgrades, testing beta versions, etc.

•             Industry knowledge: Ability to keep up with upcoming releases of related software, assess impact on platform infrastructure/user-base

Best Regards,

Arpit Arora

Pyramid Consulting, Inc.

Desk Phone: 770-255-3177 Ext 827  Email : Arpit.arora@pyramidci.com  |  Linked in: https://www.linkedin.com/in/arpit-arora-55793655  | Web:  http://www.pyramidci.com/staffing-home

We Find Hidden Talent

On 03/18/2017 11:01 PM, John Ladasky wrote:
>  To do this kind of work well, you typically want < 0.001 % transmission (10^-5) of the excitation light.  You can only achieve
> this with the fancy interference filters made of many layers of coatings.  They typically cost a few hundred dollars each, though
> some very popular wavelengths can go for less.

I used to work on a product with these kind made of furnace processed silicon/glass/metals sputtered on in high vacuum.

Won't it be nice when some self assembling nanomaterial can do this kind of light interference filtering?

I just came across a possible source of filters for working with
fluorescent dyes and such - Rosco E-Colour filters. They're made for
photography and they're dirt cheap, but what I found really neat is that
there's a sampler of all 180 colours that you can buy for 3,50 Euros,
and which features transmission curves - I've often had trouble coming
by this information when digging around for cheap alternatives to
high-end filters. See attached photo.

Has anyone worked with these, by chance?

I apologize upfront about the provocative language below ...

Unfortunately, some of the diybio is driven more by hype than any expectation of reality. 

It is a derivative of the philosophers dilemma - your confidence in achieving something is inversely proportional to your knowledge about the subject (aka the less you know the more cocky you are). That is why many enter the DIYbio community with great excitement but then lose steam when they cannot make the rapid progress they expected. Bio, DIY- or otherwise, is a grind. It's that last 20% that takes 80% of the effort.

The challenge to expanding the DIY community is to keep the excitement going but tie them to projects that have a defined end that can be achieved in a reasonable period of time. One example, developing assays to survey antibiotics in our bodies through urine or saliva, involving a global community to do the tests on themselves and creating a google map with the incidence of antibiotics in our bodies.

On Sat, Mar 18, 2017 at 8:19 AM, Pieter <pieterva...@gmail.com> wrote:

Do you remember the article "European do-it-yourself (DIY) biology: beyond hope, hype and horror" from early 2014? http://onlinelibrary.wiley.com/doi/10.1002/bies.201300149/full

"We found that all groups are driven by a core leadership of (semi-)professional people who struggle with finding lab space and equipment. Regulations on genetic modification limit what groups can do. Differences between Europe and the US are found in the distinct regulatory environments and the European emphasis on bio-art. We conclude that DIYBio Europe has so far been a responsible and transparent citizen science movement with a solid user base that will continue to grow irrespective of media attention."

For the next episode of the #OPENBIOTECH youtube series I met with co-author Günter Seyfried to discuss the most remarkable differences between now and the situation some years ago.
https://www.youtube.com/watch?v=dCVbF0ZxMC0

The conclusion that DIYBio is here to stay still holds, and it is interesting to analyse what has changed. At the time Cathal was the only with a GMO permit for his lab, now there are more but not many. The groups have developed into several different directions, resembling hacker spaces, co-working spaces or are part of larger institutions. The movement has functioned as a test-bed for startups. Collaborations with companies have also occurred (f.e. BiologiGaragen - Novozymes, La Paillasse - Roche).

Günter and I also discussed the influence of governmental support in the growth of DIYBio activities. To what extend has that made a difference?

In the same vlog you can also see a meetup with two Belgium biohackers in Brussels, with their own opinions and expectations.

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For more options, visit https://groups.google.com/d/optout.

I apologize upfront about the provocative language below ...

Unfortunately, some of the diybio is driven more by hype than any expectation of reality. 

It is a derivative of the philosophers dilemma - your confidence in achieving something is inversely proportional to your knowledge about the subject (aka the less you know the more cocky you are). That is why many enter the DIYbio community with great excitement but then lose steam when they cannot make the rapid progress they expected. Bio, DIY- or otherwise, is a grind. It's that last 20% that takes 80% of the effort.

The challenge to expanding the DIY community is to keep the excitement going but tie them to projects that have a defined end that can be achieved in a reasonable period of time. One example, developing assays to survey antibiotics in our bodies through urine or saliva, involving a global community to do the tests on themselves and creating a google map with the incidence of antibiotics in our bodies.

On Sat, Mar 18, 2017 at 8:19 AM, Pieter <pieterva...@gmail.com> wrote:

Do you remember the article "European do-it-yourself (DIY) biology: beyond hope, hype and horror" from early 2014? http://onlinelibrary.wiley.com/doi/10.1002/bies.201300149/full

"We found that all groups are driven by a core leadership of (semi-)professional people who struggle with finding lab space and equipment. Regulations on genetic modification limit what groups can do. Differences between Europe and the US are found in the distinct regulatory environments and the European emphasis on bio-art. We conclude that DIYBio Europe has so far been a responsible and transparent citizen science movement with a solid user base that will continue to grow irrespective of media attention."

For the next episode of the #OPENBIOTECH youtube series I met with co-author Günter Seyfried to discuss the most remarkable differences between now and the situation some years ago.
https://www.youtube.com/watch?v=dCVbF0ZxMC0

The conclusion that DIYBio is here to stay still holds, and it is interesting to analyse what has changed. At the time Cathal was the only with a GMO permit for his lab, now there are more but not many. The groups have developed into several different directions, resembling hacker spaces, co-working spaces or are part of larger institutions. The movement has functioned as a test-bed for startups. Collaborations with companies have also occurred (f.e. BiologiGaragen - Novozymes, La Paillasse - Roche).

Günter and I also discussed the influence of governmental support in the growth of DIYBio activities. To what extend has that made a difference?

In the same vlog you can also see a meetup with two Belgium biohackers in Brussels, with their own opinions and expectations.

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Learn more at www.diybio.org
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