Re: [DIYbio] Re: Screw cap petri dishes?

Plastic plates are polystyrene - cannot autoclave

On Sat, Jan 27, 2018 at 3:21 PM, Jo Donoghue <jomdonoghue@gmail.com> wrote:
Can you not autoclave the plastic dishes? (I thought we did in college after we poured them) If so could you not scoop out all the agar and just autoclave them and reuse them?


On Thursday, January 17, 2013 at 6:15:15 PM UTC-5, Sebastian wrote:
Anyone come across petri dishes that are autoclavable and have a screw cap or other air tight seal? I hate using plastic dishes. Land fills hate it too. Jars tend to be too space consuming. Most other vessels have space-hungry designs.

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

Sent via Mobile E-Mail

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[DIYbio] Re: Screw cap petri dishes?

Can you not autoclave the plastic dishes? (I thought we did in college after we poured them) If so could you not scoop out all the agar and just autoclave them and reuse them?


On Thursday, January 17, 2013 at 6:15:15 PM UTC-5, Sebastian wrote:
Anyone come across petri dishes that are autoclavable and have a screw cap or other air tight seal? I hate using plastic dishes. Land fills hate it too. Jars tend to be too space consuming. Most other vessels have space-hungry designs.

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

Sent via Mobile E-Mail

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[DIYbio] Screw cap petri dishes?

Help the manufacturer visualize it by designing it. Onshape is a good tool for designing something you want.

I did a sketch once for a reusable bag that could stand on its own for TC plants. It was going to save space and be flexible enough to get squeezed if one os running out of space. There would be membranes for gas premiablity etc. Even with the sketch the company rep took a half a week to tell me what is or isn't possible and how much they'd need to rearrange the manufacturing facilities to take the concept into reality.

The downside of what you ask is that it's to no ones advantage to make the product you want other than for the purpose you need. Making it reusable makes it even less likely for a company to manufacture it on a enough of a mass scale for you to find out about it.

However why not ask a company that has a preexisting form? https://discountcarcareproducts.com/products/clay-bar-box-screw-cap-top
Now it doesn't mention the volume or material but chat with them or their manufacturer. See what is doable. Let us know how it goes.

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Re: [DIYbio] Bioluminescent plants revisited

Yeah it's happening but not up to DIY bio standard. It went well above and beyond with quantum dot impregnable plant tissues.

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Re: [DIYbio] Re: BioRad ICycler Password or Service Manual

No we moved on and have a Roche 480 qpcr machine. It would be nice if Biorad would release the password since they no longer will service the icycler. Instead Biorad was committing crimes.

On Jan 26, 2018, at 7:16 AM, Gonzalo rok <ltcdata@gmail.com> wrote:

Hi!
Did you were able to get the service password?
Thanks
best regards

El miércoles, 26 de octubre de 2016, 3:41:18 (UTC-3), Roninlaw escribió:
Sadly no luck.  I haven't tried resetting the bios or using a brute force attack both are good suggestions from really good hackers. One day when I have more time I'll give it a shot. For now we have two working models in our lab in LA. 



On Oct 6, 2016, at 5:05 PM, Daniel Westcott <daniel.jam...@gmail.com> wrote:

Reviving this stale post. Did you have any luck? I just grabbed one of these from the surplus cart in our building's basement. It says Edge Fault or something. 


On Sunday, April 10, 2016 at 1:27:52 AM UTC-7, Roninlaw wrote:
Does anyone know the service option password for the BioRad ICycler or have a service manual?  I have two. One with a lid that won't heat and another with a heat block fault. I am hoping to have one good one by switching the lid. Or two if anyone has any experience or any ideas for replacing the peltiers.

Thanks,

Dan

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Re: [DIYbio] Re: BioRad ICycler Password or Service Manual

Hi!
Did you were able to get the service password?
Thanks
best regards

El miércoles, 26 de octubre de 2016, 3:41:18 (UTC-3), Roninlaw escribió:
Sadly no luck.  I haven't tried resetting the bios or using a brute force attack both are good suggestions from really good hackers. One day when I have more time I'll give it a shot. For now we have two working models in our lab in LA. 



On Oct 6, 2016, at 5:05 PM, Daniel Westcott <daniel.jam...@gmail.com> wrote:

Reviving this stale post. Did you have any luck? I just grabbed one of these from the surplus cart in our building's basement. It says Edge Fault or something. 


On Sunday, April 10, 2016 at 1:27:52 AM UTC-7, Roninlaw wrote:
Does anyone know the service option password for the BioRad ICycler or have a service manual?  I have two. One with a lid that won't heat and another with a heat block fault. I am hoping to have one good one by switching the lid. Or two if anyone has any experience or any ideas for replacing the peltiers.

Thanks,

Dan

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Re: [DIYbio] Bioluminescent plants revisited

What up doooooog!

On Wed, Jan 24, 2018 at 8:23 PM, CoreyHowe <coreyhowe99@gmail.com> wrote:
Is anyone on here interested in doing research to make glowing plants? I've been developing some proof of concept experiments but have been slow in getting my own lab space up and running. I could definitely benefit from additional people and resources. Send me a message and I can give you more details about the project.

Thanks!

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[DIYbio] Bioluminescent plants revisited

Is anyone on here interested in doing research to make glowing plants? I've been developing some proof of concept experiments but have been slow in getting my own lab space up and running. I could definitely benefit from additional people and resources. Send me a message and I can give you more details about the project.

Thanks!

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[DIYbio] Hacking an Ion Proton

Has anyone repurposed an Ion Proton DNA  sequencer for a different application?

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Re: [DIYbio] Re: Screw cap petri dishes?

On 01/21/2018 10:49 AM, JG Timourian wrote:
> Anyone come across petri dishes that are autoclavable and have a screw cap or other air tight seal? I hate using plastic dishes.

If they are air tight, would they let air out during autoclave soak, then go to more
of a vacuum inside as they cool? Seems like it would collapse a stainless sheetmetal container...

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[DIYbio] Re: Where to get project ideas for beginners

Hi Henry - if you're up for learning genetic engineering - try a starter kit : https://amino.bio/products/dna-playground-starter-set

If you're looking to learn how to grow cells, engineer them, and extract the stuff you engineered them to make, this is a good starting point - once you master this, then you can move onto a more independent project :) 



On Monday, October 9, 2017 at 7:48:35 AM UTC-6, Henry Burnett wrote:
Hi all

I am new to DIYbio and would love to get started as soon as possible. I don't know a lot about it though, so I was wondering if any of you knew where to get good, simple ideas for a first project to do? And where could I get information as to exactly how to do it (a sort of step-by-step guide as to what the actual process is when doing a project)?

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[DIYbio] Re: Screw cap petri dishes?

I don't know if you got a satisfactory answer to your question. But I thought it would be relevant to say something about my experience.

I am using a magnetic heater/stirrer and sometimes there are large lumps in the media I am stirring.  I found a product from Bel-Art that is a PS capsule to snap around the stirbar, so the bar can spin away and not be disurpted by large lumps.  I did not want to use that plastic, so I got a 60 mm diam glass petri dish, put it at bottom with its top. Drilled some holes and I get the circulation and the protection of the stir bar I want. It would be more convenient though if the lid of the petri dish could be fastened to the lower - I would not be looking for a sterile seal, but just something to keep them together.

By the way, you can get stainless steel petri dishes (rosescientific.com) so presumably they could tolerate some minor fine and brief threading to accommodate a screw type lid.

On Thursday, January 17, 2013 at 6:15:15 PM UTC-5, Sebastian wrote:
Anyone come across petri dishes that are autoclavable and have a screw cap or other air tight seal? I hate using plastic dishes. Land fills hate it too. Jars tend to be too space consuming. Most other vessels have space-hungry designs.

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

Sent via Mobile E-Mail

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[DIYbio] grow meat at home

Hey ! 

I look after ressources explaining how to grow meat at home. but it's hard to find something relevant.

shojinmeat  give some secondary school workshop but their wiki is in japonese. I guess it's easier to grown meat than learn japonese.
do you have some like to share on this topic ?

Any help would be appreciate.Thanx ! 
Best regards ! 

Loïc

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New Position-SAP Treasury Operations

Dear Partner,

Hope you are doing great!!

Please go through the requirement and let me know if you are having any consultant for this position.

Please share me the profile asap as this is a very hot requirement.

SAP Treasury Operations

Sunnyvale, CA

6+ Months

 

Description/Comment: SAP Banking; Treasury; SAP OperationsExecute and manage bank account openings/services required for In-House Bank (IHB) region, primary owner for specific IHB region and provide secondary support for other IHB regionsSupport the integration of bank service components required for SAP (i.e. bank file exchanges, cash management services required from each bank account)Analyze and monitor new bank fees associated with IHB for all bank accountDevelop and maintain bank account mappings by country.Ensure deployment of funding mechanisms are aligned with intercompany agreementsAssist with managing weekly discussions with IHB banks, cross functional /internal Treasury teamsAdditional Job Details:

 

 

Thanks,

                                                                                                                                                                         

Arpit Arora

Pyramid Consulting, Inc.

Executive-Resourcing

 


Desk Phone: 415.943.9386 E: Arpit.arora@pyramidci.com  Web: www.pyramidci.com

 

cid:image009.jpg@01D02C05.4FFC5560

 

WE FIND HIDDEN TALENT

 

How am I doing ?   My goal is to provide you with excellent service. If you have questions, suggestions, feedback about your experience, or need to escalate an issue, feel free to reach out to my manager via email at Mohammed.Tausheef@pyramidci.com or via phone at 415.943.9384.

 

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Re: [DIYbio] Re: Were the DIY CRISPR kits contaminated?

On Tuesday, December 26, 2017 at 9:01:47 AM UTC-5, Wolfgang Nellen wrote:

Did you get any response from the Germans yet?
Concerning communication: is there anything like a Facebook site where all communities could talk to each other and discuss issues like the kit? I guess the authorities do not have a clue where the DIY communities are!


Hi,

        There are some. Try https://www.facebook.com/groups/diybio/ and https://www.facebook.com/groups/769326966539324/ . This might be relevant too: https://www.facebook.com/groups/biohackacademy/ 

        Also, if you are into fungi these might be sort of relevant: https://www.facebook.com/groups/FungalSequencing/ & https://www.facebook.com/groups/fungalmaterials/

        There are many other possible matches.

August


 
On 22 Dec 2017 20:00, "Vanessa lorenzo toquero" <vloren...@gmail.com> wrote:
Hi,

I introduce myself. Vanessa Lorenzo from Hackuarium biohackerspace in Renens, Switzerland, co-leading it together with Rachel Aronoff and the board,

Just to inform that we had a kit from the Odin at the lab. We decided to get in contact with the German authorities who suggested that our kit might also be contaminated. We sent them the kit.

We also got better informed on how our community could contribute
better to the DIY Bio movement. A more dynamic dialogue between the authorities and the affected peers of the DIY Bio movement would had been more desirable but we are now navigating between different landscapes with different time scales and communication protocols. We should push for a better more transparent ones, and of course our door is literally transparent and always open on Wednesdays or on appointment (basically cause we are volunteers and have side jobs or even lives!). Having a constructive and responsible attitude towards the issues we face could help shaping the science we dream of.

Best regards

Vanessa

El viernes, 22 de diciembre de 2017, 9:52:09 (UTC+1), Jonathan Ferooz escribió:
Nathan I think you are wrong, in Belgium we are very interested to collaborate with other groups (and I'm sure in other EU countries too). But in contrast with some other groups we are a small group with no funding.
What do you think we can do?
We are pushing, at our level, to show what is the DIYbio and hope that we'll can adapt our laws to facilitate the DIYbio.
In europe just have the right to put GFP in a genome it's a fight with the authorities.
If other groups want to collaborate with us in Belgium, we are open.
We are not making the show on TV, Nature or other, but yes, we are there.

Le jeudi 21 décembre 2017 23:20:55 UTC+1, Nathan McCorkle a écrit :




I am surprised this has got any more attention, given the lack of response the EU biohackers have provided here on this mailing list. It gives the impression that EU biohackers are not interested in collaboration, and are making noise for some self-serving goals. This behavior only makes me wary of EU biohackers. Very sad.


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Re: [DIYbio] CRISPR to make just males

They did it without FBS but no patents that I could find so probably not very successful. University of Amsterdam, Dr. Klaas Hellingwerf was in charge of growth media and he moved to other things. 
I did find cellastim recombinant albumin and sericin from silkworm cocoon https://www.nature.com/articles/srep31516 that look promising.  

On Friday, January 12, 2018 at 2:27:46 AM UTC-6, Patrik D'haeseleer wrote:
On Thursday, January 11, 2018 at 4:36:25 PM UTC-8, Gordana Ostojic wrote:
Again, question of top - down or bottom - up. This is an old chemistry/mat sci question.

Anyway, does anyone know about bioengineering of fetal bovine serum equivalent? 

Mark Post, who developed the first "Cultured Beef" burger, told me that they did develop a good animal-free growth medium for the bovine muscle cells they're growing. Don't know the details, but I wouldn't be surprised if there was a patent out there somewhere...

Patrik

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Re: [DIYbio] CRISPR to make just males

I remember seeing ads for recombinant FBS years ago while working in a regular lab, I'm sure it exists already? The draw for overfunded labs is consistency and reproducibility, of course, but in principle it should be cheaper if done at scale, too.

On 12 January 2018 08:27:46 GMT+00:00, Patrik D'haeseleer <patrikd@gmail.com> wrote:
On Thursday, January 11, 2018 at 4:36:25 PM UTC-8, Gordana Ostojic wrote:
Again, question of top - down or bottom - up. This is an old chemistry/mat sci question.

Anyway, does anyone know about bioengineering of fetal bovine serum equivalent? 

Mark Post, who developed the first "Cultured Beef" burger, told me that they did develop a good animal-free growth medium for the bovine muscle cells they're growing. Don't know the details, but I wouldn't be surprised if there was a patent out there somewhere...

Patrik


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Re: [DIYbio] CRISPR to make just males

On Thursday, January 11, 2018 at 4:36:25 PM UTC-8, Gordana Ostojic wrote:
Again, question of top - down or bottom - up. This is an old chemistry/mat sci question.

Anyway, does anyone know about bioengineering of fetal bovine serum equivalent? 

Mark Post, who developed the first "Cultured Beef" burger, told me that they did develop a good animal-free growth medium for the bovine muscle cells they're growing. Don't know the details, but I wouldn't be surprised if there was a patent out there somewhere...

Patrik

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Re: [DIYbio] CRISPR to make just males

Does not answer your question directly but Eric Espinosa introduced us to FetalGro at BioCurious, it is bovine serum based but substantially cheaper than fetal bovine serum.

On Thu, Jan 11, 2018 at 4:36 PM, Gordana Ostojic <gordana.n.ostojic@gmail.com> wrote:
Again, question of top - down or bottom - up. This is an old chemistry/mat sci question.

Anyway, does anyone know about bioengineering of fetal bovine serum equivalent? 

On Thursday, January 11, 2018 at 6:20:04 PM UTC-6, Nathan McCorkle wrote:


On Jan 11, 2018 4:01 PM, "Gordana Ostojic" <gordana....@gmail.com> wrote:
And of course the best would be to finally get meat-growing from tissue culture (maybe diybio can work on bacterial production of growth factors?).

Or cattle without brains.

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Re: [DIYbio] CRISPR to make just males

Again, question of top - down or bottom - up. This is an old chemistry/mat sci question.

Anyway, does anyone know about bioengineering of fetal bovine serum equivalent? 

On Thursday, January 11, 2018 at 6:20:04 PM UTC-6, Nathan McCorkle wrote:


On Jan 11, 2018 4:01 PM, "Gordana Ostojic" <gordana....@gmail.com> wrote:
And of course the best would be to finally get meat-growing from tissue culture (maybe diybio can work on bacterial production of growth factors?).

Or cattle without brains.

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Re: [DIYbio] CRISPR to make just males



On Jan 11, 2018 4:01 PM, "Gordana Ostojic" <gordana.n.ostojic@gmail.com> wrote:
And of course the best would be to finally get meat-growing from tissue culture (maybe diybio can work on bacterial production of growth factors?).

Or cattle without brains.

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Re: [DIYbio] CRISPR to make just males

And of course the best would be to finally get meat-growing from tissue culture (maybe diybio can work on bacterial production of growth factors?). Not to mention that doing work there would help growing organs for people.

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[DIYbio] connectors and cables on lab gear.

I'm working on the culture shock electroporator that uses power and needs serial data connection
when you want to download shot data, or upgrade it's firmware. When developing code in micropython for it,
it can be helpful to control it from a USB cable 3 feet long.

So, I'd like to know what you think is best, micro USB 2.0 type B plugs as are used in many cellphones to charge them,
or newer USB 3.1 type C plugs that don't have an orientation -- they can be flipped over and work the same.

Next question is about having one used for power only -- I'm thinking maybe one could be type C and one type B so there
would never be confusion if plugged in the wrong port. The electroporator will have a power port and a serial port.

If you allow both to be the newer type C, you get benefits and potential problems such as serial cable plugged to the power port
and no serial data, seems dead. The benefits of type C connectors are longer life until wear out, easier to use
since you just push it in, they are going to be a standard soon, and they can be splash resistant, even waterproof.

Any thoughts on trends appreciated.

The near future could allow wireless data and near field magnetic charging, but no just yet...
For the first culture shock iteration, cables are going to be used part time even if zapping on battery power.

--
John Griessen
blog.kitmatic.com Austin TX building lab gear for biologists

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Re: [DIYbio] CRISPR to make just males

More interesting would be to do the same to Birds to get female-only laying hens, and perhaps spare 50% of chicks from immediate murder for being born the wrong sex.

On 11 January 2018 20:36:47 GMT+00:00, Abizar Lakdawalla <abizarl@gmail.com> wrote:

Just Boy Cattle

The University of California, Davis' Alison Van Eenennaam is using CRISPR to develop all-male cattle, Technology Review reports.

Her goal, it adds, is to develop bulls that will only sire male offspring in a project she's dubbed "Boys Only." That, Van Eenennaam says, would help beef ranchers out as males are bigger and grow faster and would make the industry more efficient.

She and her lab are going about it by inserting the SRY gene, which is usually found on the Y chromosome, on the X chromosome, Tech Review says. In mammals, that gene, also called testis-determining factor, can make an animal physically male, even without a Y chromosome, it adds.

So far, Tech Review reports that Van Eenennaam and her team have been able to add SRY to an X chromosome in male skin cells, and that they next have to tackle doing that in a cattle embryo or cloning that altered skin cell. Any males made in this way are expected to be sterile, it notes.

Van Eenennaam has previously worked on developing hornless dairy cows by splicing a gene from Angus cattle, which naturally lack horns, into dairy-producing Holsteins.


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[DIYbio] CRISPR to make just males

Just Boy Cattle

The University of California, Davis' Alison Van Eenennaam is using CRISPR to develop all-male cattle, Technology Review reports.

Her goal, it adds, is to develop bulls that will only sire male offspring in a project she's dubbed "Boys Only." That, Van Eenennaam says, would help beef ranchers out as males are bigger and grow faster and would make the industry more efficient.

She and her lab are going about it by inserting the SRY gene, which is usually found on the Y chromosome, on the X chromosome, Tech Review says. In mammals, that gene, also called testis-determining factor, can make an animal physically male, even without a Y chromosome, it adds.

So far, Tech Review reports that Van Eenennaam and her team have been able to add SRY to an X chromosome in male skin cells, and that they next have to tackle doing that in a cattle embryo or cloning that altered skin cell. Any males made in this way are expected to be sterile, it notes.

Van Eenennaam has previously worked on developing hornless dairy cows by splicing a gene from Angus cattle, which naturally lack horns, into dairy-producing Holsteins.

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Re: [DIYbio] Re: Experience with tree genetics?

        Even if I write something stupid it might just become the impetus for someone else to jump in do better (if only to provide a correction), right?


On Wed, Jan 10, 2018 at 4:59 PM, Jo Donoghue <jomdonoghue@gmail.com> wrote:
Thanks for the starting help!!


On Monday, January 8, 2018 at 10:53:18 PM UTC-5, August Pamplona wrote:
On Monday, January 8, 2018 at 11:41:22 AM UTC-5, Jo Donoghue wrote:
I recently came across a problem we are having in my city where all our Maple trees are made from tissue cultures and do not have much genetic diversity. They plan on using seeds from wild maples to redo the area around the city. They have sited that the Asian longhorned beetle would still be a problem to most maples. My goal would be to take whatever the natural deterrent is in cedar and put it in the genome of these wild bred maples. Does anyone have a suggestion as to the deterrent or the gene I'm looking for? My guess is it is the scent of the cedar which may have multiple loci.


        I would say that "whatever the natural deterrent is in cedar" is pretty vague (but you know that). My guess is that you would be dealing with many genes in isoprenoid biosynthetic pathways (many both because it may require more than one substance being produced and because it would take many different enzymes to produce a given substance of interest).

        Have you done a literature search? Maybe start with papers with titles that look like the following and then look at papers cited in the references and at papers citing whatever you find, etc..

 

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[DIYbio] Re: Experience with tree genetics?

Thanks for the starting help!!


On Monday, January 8, 2018 at 10:53:18 PM UTC-5, August Pamplona wrote:
On Monday, January 8, 2018 at 11:41:22 AM UTC-5, Jo Donoghue wrote:
I recently came across a problem we are having in my city where all our Maple trees are made from tissue cultures and do not have much genetic diversity. They plan on using seeds from wild maples to redo the area around the city. They have sited that the Asian longhorned beetle would still be a problem to most maples. My goal would be to take whatever the natural deterrent is in cedar and put it in the genome of these wild bred maples. Does anyone have a suggestion as to the deterrent or the gene I'm looking for? My guess is it is the scent of the cedar which may have multiple loci.


        I would say that "whatever the natural deterrent is in cedar" is pretty vague (but you know that). My guess is that you would be dealing with many genes in isoprenoid biosynthetic pathways (many both because it may require more than one substance being produced and because it would take many different enzymes to produce a given substance of interest).

        Have you done a literature search? Maybe start with papers with titles that look like the following and then look at papers cited in the references and at papers citing whatever you find, etc..

 

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[DIYbio] Re: Experience with tree genetics?

On Monday, January 8, 2018 at 11:41:22 AM UTC-5, Jo Donoghue wrote:
I recently came across a problem we are having in my city where all our Maple trees are made from tissue cultures and do not have much genetic diversity. They plan on using seeds from wild maples to redo the area around the city. They have sited that the Asian longhorned beetle would still be a problem to most maples. My goal would be to take whatever the natural deterrent is in cedar and put it in the genome of these wild bred maples. Does anyone have a suggestion as to the deterrent or the gene I'm looking for? My guess is it is the scent of the cedar which may have multiple loci.


        I would say that "whatever the natural deterrent is in cedar" is pretty vague (but you know that). My guess is that you would be dealing with many genes in isoprenoid biosynthetic pathways (many both because it may require more than one substance being produced and because it would take many different enzymes to produce a given substance of interest).

        Have you done a literature search? Maybe start with papers with titles that look like the following and then look at papers cited in the references and at papers citing whatever you find, etc..

http://sci-hub.tw/10.1007/s11101-006-0001-6
http://sci-hub.tw/10.1111/j.1744-7909.2010.00910.x
 

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Re: [DIYbio] Re: 4-tube incubator and optical density meter arduino shield

One thing I don't quite understand from this. Do they get the liquid handling root to remove the lid of the turbidometer to add more media?

If you are looking for one that dosen't need a robot there is the flexostat.
https://depts.washington.edu/soslab/turbidostat/pmwiki/pmwiki.php?n=Main.News

They also use tsl230, but 2 per chamber, and use a 650nm laser rather than LED.

Cheers,
Brian

On Thu, Jun 29, 2017 at 2:37 AM, Simon Quellen Field <sfield@scitoys.com> wrote:

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On Wed, Jun 28, 2017 at 5:34 PM, Evgeny Vlasov <vlasov.ca@gmail.com> wrote:
Hi!
That is one very interesting link, exactly what I was looking about. Thank you so much.
Link itself is broken but .pdf is searchable. Tomorrow I am going to nag lab's bacteria guy with this project. 

Cheers

Evgeny


On Thursday, October 7, 2010 at 4:34:43 PM UTC-6, ByoWired wrote:


On Oct 7, 5:46 pm, Mackenzie Cowell <m...@diybio.org> wrote:
> I'd like to build a simple incubator+OD meter for up to 4 microcentrifuge
> tubes....

My first quick comment is that you might have a look at the following:

http://soslab.ee.washington.edu/mw/images/e/ef/EE449_SP10_Group6_MS5_Report.pdf

In that, they used LEDs and a Light-to-Frequency Converter called a
TSL230 which I think they interfaced with the Arduino.  They seem
confident in their results.
I'm in the process now of testing an OD system using a similar set-up,
but it's taking OD readings and some other optical properties through
a syringe, not microcentrifuge tubes.  Also, I'm not familiar with the
Arduino and have built my system around the Propeller microcontroller
- sorry.  I have an incubator built around the syringes using non-
inductive resistors, but I haven't yet tested the incubator portion
yet.  I have digital thermometers using DS18B20's.  Although I've
heard of people using PCB traces for heating, it seems to me it might
be easier to use resistors because they can be positioned around the
microtubes without dramas.

Anyway that's it for now.
hope that helps get you started,
Mark

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