Hi Cathal - the Sciencehackday wiki has events listed around the
world, including one being planned fro Dublin:
http://sciencehackday.pbworks.com/w/page/43932719/Dublin
I was involved in the "Hack your genome" and "DNAquiri" hacks. We
still need to do a bit more complete writeup at some point though. The
DNAquiri hack actually won Best In Show, although bribing the judges
with liquor may have had something to do with that :-D
For the DNAquiri, freeze-thaw and a brief heat cycle was plenty to
lyse the cells - no detergent needed. You don't need too much salt,
and sweet fruit will easily mask the salt. Strawberries gave by far
the most DNA, possibly because the commercial varieties are octoploid.
We had a big pack of frozen strawberries, which is just perfect. We
also used a fresh pineapple and a can of frozen pineapple juice for
protease, but I'm not sure that made much difference. We wound up
layering different fruit pulps, with the strawberry on top because
that's the layer that generates most DNA.
You do need to use a high-proof alcohol to get good DNA extraction. We
used a bottle of Bacardi 151 and one of Everclear (both 151 proof, or
75%). Using this combination, we got a LOT of DNA in the top of the
glass. You also get a layer of really strong alcohol at the top, so
you may want to stir in the alcohol before drinking. If you let the
glass stand a bit, you'll keep extracting more DNA, but after half an
hour or so it does start to degrade noticeably. We poured the alcohol
during the judging, so by the end the little shot glasses we had set
out for the audience weren't quite as snotty anymore as we'd hoped.
To be honest, the texture really wasn't a problem at all. If anything,
the fact that we hadn't strained the fruit pulp was more of an issue -
we had just smashed up the fruit by hand in a plastic bag instead of
blending it, to avoid shearing the DNA. But that did mean some of our
cocktails were a little lumpy. Taste was pretty good though. It's
essentially just fruit pulp, high-proof alcohol, and a small pinch of
salt.
I forgot to bring my camera, but here's some pics I found online from
other people, with the idea of maybe putting together an instructable:
http://t.co/sl0NgY6A
http://t.co/0T8aU6cL
http://t.co/s2lHyqDr
http://t.co/cjMv3ToW
http://www.flickr.com/photos/mbiddulph/6338424461/
http://www.flickr.com/photos/sfbrightworks/6345720168/
http://www.flickr.com/photos/sfbrightworks/6345722136/
http://www.flickr.com/photos/sfbrightworks/6345719194/
http://www.flickr.com/photos/junnibug/6341329023/
On Nov 17, 8:58 am, Cathal Garvey <cathalgar...@gmail.com> wrote:
> Hey all,
> If you (like me) weren't attending the SF-SciHackday, then you might
> like to peruse the list of hacks/projects to see what people got up to.
> Looks like a lot of fun was had, and there are a bunch of familiar names
> there!http://sciencehackday.pbworks.com/w/page/47743279/sfhacks2011
>
> A few particularly DIYbio projects to look out for:
>
> "Save the Cardinal!", Software to assist an ornithology citizen science
> project:http://sciencehackday.pbworks.com/w/page/47743279/sfhacks2011#hack_3
>
> "Homebrew Microscope", a sub-micron DIY microscope using plumbing parts
> and a camera:http://sciencehackday.pbworks.com/w/page/47743279/sfhacks2011#hack_9
>
> "HIV/AIDS: Meaningful Indicators?", using machine-learning to seek out
> cryptic cues in UNAIDS data that could inform better epidemiology:http://sciencehackday.pbworks.com/w/page/47743279/sfhacks2011#hack_10
>
> "EpiCell", mapping cell phone usage versus healthcare availability to
> determine areas where cell-based epidemiology would be most effectively
> applied:http://sciencehackday.pbworks.com/w/page/47743279/sfhacks2011#hack_13
>
> "Hack Your Genome", a code project to visualise and explore 23andMe data:http://sciencehackday.pbworks.com/w/page/47743279/sfhacks2011#hack_22
>
> A project to search for semantic cues in research papers indicating that
> the author has a lot of confidence or excitement about his work, to mine
> for early publications worth reading:http://sciencehackday.pbworks.com/w/page/47743279/sfhacks2011#hack_23
>
> "DNAquiri", a Surfacant-free DNA extraction for DNA-cocktails:http://sciencehackday.pbworks.com/w/page/47743279/sfhacks2011#hack_15
>
> Can't wait to get me to a hackday sometime soon. Any Euro-Hackdays planned?
>
> Cathal
>
> --www.indiebiotech.com
> twitter.com/onetruecathal
> joindiaspora.com/u/cathalgarvey
> PGP Public Key:http://bit.ly/CathalGKey
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