Very cost effective/sensitive. You can use 6x primers, and reaction setup is same as regular PCR except you use Bst polymerase. You can also check for product in the tube by adding a dsDNA dye such as SYBR green or even SYBR safe - visually (!). Most importantly, you only need a isothermal incubation - so no cycling.
2 problems:
1) everything remotely connected to LAMP is heavily patented. Specific assays, reporting mechanisms, devices etc.
2) cannot replace PCR as you are amplifying a "chain" DNA structure, not your target. So its great for diagnostics but no application to building plasmids etc.
Ravi
On Monday, July 2, 2012 5:59:13 AM UTC-4, Marc Dusseiller wrote:
hei garage biologists,just saw some interesting low-cost stuff at a conference in kenya. you know about LAMP? seems like a great and cheap alternative to pcr, especially on the instrumentation side.http://www.plosntds.org/article/info%3Adoi%2F10.1371% 2Fjournal.pntd.0000147 more stuff from low-cost diagnostics soon,marc
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