Re: [DIYbio] check LAMP! pcr is soooooo 90ies..

> isothermal temp controlled device

= Cup of Coffee? :)

On 03/07/12 17:40, Nathan McCorkle wrote:
> Cost is comparable to PCR, 3x amount of primers, Bst polymerase from NEB,
> isothermal temp controlled device
> On Jul 3, 2012 4:09 AM, "Pieter" <pietervanboheemen@gmail.com> wrote:
>
>> The papers I've seen on LAMP look very promissing. Especially this one on
>> malaria:
>> http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0013733
>>
>> I'd like to read some more about the inhibitory effects that have been
>> described when samples are not completely purified. I can imagine that 6
>> primers make the reaction more sensitive to polutants. Also the economic
>> performance of the method is unclear to me (e.g. how much does it cost?).
>>
>> Does anyone have a good reference to an article where these parameters are
>> studied? I'd love to learn more about it!
>>
>> On Monday, 2 July 2012 15:22:14 UTC+2, Cathal wrote:
>>>
>>> LAMP is an awesome method for on-site PCR, but (speaking as someone who
>>> *hasn't* tried it) seems to be harder to optimise and design for
>>> general-purpose DNA amplification.
>>>
>>> That is, standard PCR is better for prototyping a reaction, but for
>>> creating a protocol for general or regular use, LAMP is probably a good
>>> next step; as you point out, it requires little in the way of
>>> instrumentation, and because there's no cycling of temperature, that's a
>>> huge source of experimental error removed! Awesome for walk-by workshops
>>> for DNA testing etc..
>>>
>>> On 02/07/12 10:59, Marc Dusseiller wrote:
>>>> hei garage biologists,
>>>>
>>>> just saw some interesting low-cost stuff at a conference in kenya. you
>>> know
>>>> about LAMP? seems like a great and cheap alternative to pcr, especially
>>> on
>>>> the instrumentation side.
>>>>
>>>> http://www.plosntds.org/**article/info%3Adoi%2F10.1371%**
>>> 2Fjournal.pntd.0000147<http://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0000147>
>>>>
>>>> more stuff from low-cost diagnostics soon,
>>>> marc
>>>>
>>>
>>>
>>> --
>>> www.indiebiotech.com
>>> twitter.com/onetruecathal
>>> joindiaspora.com/u/**cathalgarvey<http://joindiaspora.com/u/cathalgarvey>
>>> PGP Public Key: http://bit.ly/CathalGKey
>>>
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>


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