Much more better maps are shipped with free samples - with restriction sites, with genes location. And yes as it says Mega, Hepatitis B genes can be cutted off with restriction enzymes(If I had them) and plasmide can be used in other way - Aldevron writes it in papers, shipped with plasmids.
понедельник, 2 июля 2012 г., 18:22:37 UTC+3 пользователь Nathan McCorkle написал:
Those plasmid maps really, really suck... it's great to see the--
restriction sites, but for anyone here that may ever label a plasmid
map, please add the intentional features as well (ori, selection
markers, MCS, promoter)
Shamrock is right that this is meant for mammalian cell line use, so
while it may replicate in E.coli, it probably isn't much fun. That
said, I don't think there's any problem with a protein, or fragment
thereof, being derived from Hepatitis B... it's not the whole virus,
so it can't do too much other than maybe stimulate an immune response
(which may or may not be severe)
On Sat, Jun 30, 2012 at 7:03 AM, Александр Дориф <dorif11@gmail.com> wrote:
> Hello! I have found the way to get some free plasmids with map and work
> protocol. The Aldevron company distributes free samples of pRc/CMV-Hbs(S)
> and pCMVHB-S2.S plasmids. Both are based on pBlueScript plasmid and contain
> Ampicilline resistance gene. Each sample contains 2-4 ug of plasmid DNA.
> Samples are delivered via postage service. Link to order
> samples: http://www.aldevron.com/products/dnas/plasmids/
>
> I hope it'll be useful for somebody.
>
> P.S.: sorry for my bad English.)
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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