Re: [DIYbio] Re: Translating bacterial DNA into plant DNA

No, they're *transcriptional* terminators. They're essentially what makes the difference between having an operon with two co-transcribed genes, versus two independently expressed genes in series.

If you made a little genetic circuit with 4-5 genes that all have their own promoter and regulators, last thing you want is for gene B to accidentally get turned on by read-through transcription from gene A because you forgot to put a terminator in between. They essentially insulate one transcriptional unit from another.

It's kinda like when you're deigning a printed circuit board, you have to make sure the copper traces are separated far enough from each other. Because otherwise you might accidentally get some crosstalk between different signals, and the circuit may start to behave in ways you totally hadn't intended.


On Friday, September 28, 2012 3:49:30 AM UTC-7, Mega wrote:

Ok,
Thank you!!  If the termination doesn't work you'll get fusion proteins thus. If you place the other protein in frame, of course. Great.

On Fri, Sep 28, 2012 at 12:45 PM, Patrik D'haeseleer <pat...@gmail.com> wrote:

I think it mainly gives you a more reliable termination. Terminators are always "leaky" to some extent. Putting two in a row guarantees you won't get read-through into the next gene downstream, which could cause all sorts of crosstalk in whatever genetic circuit you're designing.

On Friday, September 28, 2012 2:25:30 AM UTC-7, Mega wrote:

Hi, 

I saw that IGEM uses a double terminator sometimes. Does that give you better protein expression?

 

http://partsregistry.org/Part:BBa_B0014

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