Re: [DIYbio] SpectralWorkbench.org & diy spectrometers

For that to be useful, the pigments would have to have a sharp change in
absorbance/emission upon binding to DNA, or you'd have to carefully wash
away unbound pigment without altering the quantities of DNA you're
trying to measure.

Ethidium bromide, as usual, exhibits the desired behaviour, becoming
much brighter upon binding DNA; that's the basis behind the old agarose
spot-test where you just dot some DNA on an agarose gel with some EtBr
in it and observe how bright it gets. I think. I never did it. I've even
heard of people just mixing known amounts of EtBr solution directly with
DNA and measuring fluorescence.

Do other dyes work the same way? Would Sybr-Safe work this way? If so
it'd be a nice solution like Mac suggests.

On 05/09/12 18:57, Mackenzie Cowell wrote:
> Dakota, what about using DNA/protein binding pigments to downshift the
> spectrum? An interim solution until someone develops a low-cost spec.
>
> Mac
>
> On Tue, Sep 4, 2012 at 7:22 PM, Dakota <dkotes@gmail.com> wrote:
>
>> It is a very well put together looking project but we still need a UV
>> 260nm and 280nm for DNA and protein quantification
>>
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