Heh - I think we'd need something like a set of all 4-nucleotide TAL effector nucleases: pick a random 3-mer TALEN, cut up the target sequence in fragments around 64 bp long, and compare the restriction fragment length pattern against the expected pattern.
In the end, considering that DNA sequencing is orders of magnitude cheaper than synthesis, I assume that sequencing will be our validation of choice. With all the super-high-throughput NGS sequencers these days, I think there's still a real market opportunity for a small and cheap device to do single amplicon sequencing. I'd *love* to have a little desktop device that does a single Sanger sequence at 1/10th the cost.
On Monday, October 1, 2012 3:05:33 PM UTC-7, Cathal wrote:
Key signing and hash verification etc. are all well and good, but they--
don't mean anything until you can verify that the hashed-and-signed
digital representation actually matches the DNA.
To meaningfully confirm the content of a DNA strand calls for a DNA
hashing method; DNA barcoding is a step in this direction for whole
species, but a method for smaller molecules that generates useful and
visibly distinct output would be needed to "hash" plasmids.
On 01/10/12 22:57, Bryan Bishop wrote:
> On Mon, Oct 1, 2012 at 4:51 PM, Nathan McCorkle <nmz...@gmail.com> wrote:
>
>> I would actually worry more about physical DNA being faked or 'malwared',
>> so I'm a propenent of keeping the repo digital. We should start by
>> mirroring the existing registry(s).
>
>
> I am surprised Marc hasn't setup public key signing of the DNA samples from
> Biofab yet. Hmm. This is a probably a thing that we should do.
>
> - Bryan
> http://heybryan.org/
> 1 512 203 0507
>
--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey
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