Monday, November 5, 2012

Re: [DIYbio] Re: Any ideas on edible electrophoresis buffers?

Would a clear soda like sprite have enough salt ions to work? Would be cool if it worked. Im guessing the KCL used in your buffer was a multimolar solution? Either way, awesome hack. The stack of silverware as a comb was brilliant! :)

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

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On Nov 5, 2012, at 7:26 PM, "Patrik D'haeseleer" <patrikd@gmail.com> wrote:

We wound up wasting a lot of time on the first day trying to get electrophoresis to work on gelatin gels. We actually did get a tiny bit of migration of the food dyes with a very solid gel (think "ballistics gel" rather than jello!). But at lower gelatin concentrations that should have given better migration into the gel, and would have been far more palatable, the gels kept melting as soon as we applied current.

Joseph Elsbernd (@CodonAUG) wound up bring much of his home lab to Science Hack Day, so we did much of the initial experimentation on that, while we worked to build a gel electrophoresis apparatus from scratch:

<image.jpeg>

And how's this for a gel comb hack. Yes, I know there are far easier ways to make a gel comb, but this was more fun, and created lovely slated wells:

<image.jpeg>

Patrik

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