Would a clear soda like sprite have enough salt ions to work? Would be cool if it worked. Im guessing the KCL used in your buffer was a multimolar solution?
Sprite would probably work.Our solution was only very slightly salty to the taste, and I know most sodas include salts as taste enhancers. No idea what the carbonation will do though.
Also, we didn't really care about buffering capacity of our "buffer", since we weren't woking with proteins or DNA where pH control really matters.
Joseph wrote down all our recipes/protocols, so I'll wait for him to post those somewhere.
Patrik
--Either way, awesome hack. The stack of silverware as a comb was brilliant! :)Sebastian S CociobaCEO & FounderNew York Botanics, LLCSent via Mobile E-MailWe wound up wasting a lot of time on the first day trying to get electrophoresis to work on gelatin gels. We actually did get a tiny bit of migration of the food dyes with a very solid gel (think "ballistics gel" rather than jello!). But at lower gelatin concentrations that should have given better migration into the gel, and would have been far more palatable, the gels kept melting as soon as we applied current.
Joseph Elsbernd (@CodonAUG) wound up bring much of his home lab to Science Hack Day, so we did much of the initial experimentation on that, while we worked to build a gel electrophoresis apparatus from scratch:
<image.jpeg>
And how's this for a gel comb hack. Yes, I know there are far easier ways to make a gel comb, but this was more fun, and created lovely slated wells:
<image.jpeg>
Patrik
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