Did you ever make a glowing bonsai tree? I would love to see pictures if you got them!
Unfortunately, not. Quite difficult, wanted to get a chloroplast integration plasmid once, but no lab wanted to provide me with it. That would have been quite easy, PCR -> lux genes, kanamycin resistance, -> into chloroplasts and they glow.
You can get one synthesized though. Costs ~0,35$ per base pair, makes for the chloroplast integration backbone ~ 1000 $....
You could also do 6 PCRs from ususal chloroplast DNA to make the vector, and ligate them together. That would be cheaper, but more difficult though.
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