we did the point mutation on eGFP optimized for bacterial expression with the induced vs. non-induced controls and coelenterazine as the positive control with purified Gaussia luciferase from a mammalian expression system. did not work as expected in our hands.
the ability to get this would revolutionize non invasive imaging since you wouldn't need to inject any luciferin/coelenterazine, so of it did work it would have been a big paper by the inventors. the most worrisome thing to me is they appear to have dropped the project.
jordan
But why did they patent it then?Jordan, it would be interesting how you did that... Did you take wild-type GFP and make a point mutation? Or enhaced GFP?Have you used the very strongest promoter (maybe the luminescence was too weak to be mesaured?) ?P.S. "Sciencenewstop" is not part of google, is it? I just found this... They didn't even care about deleting email adresses cited...--
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