Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions

On Fri, Jan 04, 2013 at 02:13:25PM -0800, Josiah Zayner wrote:
> If something is highly viscous try and use a centrifugation step before you
> filter or even Wattman paper. I have used those filter you found and they
> will be clogged just as fast as a syringe filter.

The solution itself is viscous. There's another complication:
the components (ethanediol/Me2SO) might well destroy the
filtering membrane.

I just checked http://www.spectrumlabs.com/dialysis/Compatibility.html
and it seems limited exposure will be doable.

However, the solutions themselves have turned out to be
not autoclavlable, so we'll have to settle to add a few
ml injection-grade water to bottles and autoclave these,
then sterile-filter and store under vacuum in the fridge.

> On Tuesday, January 1, 2013 5:09:32 AM UTC-6, eleitl wrote:
> >
> > On Mon, Dec 31, 2012 at 01:08:27PM -0500, Avery louie wrote:
> > > They also make filters to go on bottles:
> > >
> > >
> > http://fishersci.com/ecomm/servlet/fsproductdetail_10652_12704331_29104_-1_0?LBCID=26030796
> >
> > Thanks, these look just the ticket. Water jet vacuum with a sterile
> > syringe membrane
> > in between to prevent contamination and sucking back of liquid, and we're
> > in business.
> >
> >
>
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