Re: [DIYbio] What are some good, hydrophobic solvents I can LEGALLY buy?

That's interesting but like I said, how would you be able to manipulate the fluid inside of there to do extractions? Wouldn't the CO2 boil/explode the second I open it? Assuming that thing doesn't explode, the CO2 would just quickly boil away and the extracts I captured with it would fall back into the aqueous phase. Then I'm back where I started. I don't think it would be practical.

I'm swinging back and forth between DCM and butane.

On Saturday, January 5, 2013 8:33:59 PM UTC-5, Simon Field wrote:

Carbon dioxide becomes a supercritical fluid above 27 Celsius and above 1080 psi.

You can get tanks good for that pressure for less than $80.

But there is a much simpler way. If you have a machine shop, you can go more upscale.

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On Sat, Jan 5, 2013 at 5:05 PM, Nathan McCorkle <nmz...@gmail.com> wrote:

Maybe Simon or someone could weigh in on if this is easily DIYable,
but supercritical CO2 is a great Non-Polar (NP) solvent.

Depending on how edible/toxic you want the stuff to be (i.e. you're
adding it to some cells down the line vs just doing spectroscopy), I
know of quite a few easily obtainable solvents found at camping or
home repair (hardware) or automotive stores.

Gasoline, diesel, kerosene, ethanol, methanol, limonene (sold as 100%
citrus extract air freshener), Methy Ethyl Ketone, Acetone, Naptha,
Toluene, Xylene, Mineral Spirits, White Gas (camping), Brake Parts
Cleaner (acetone, toluene, methanol, CO2)

On Sat, Jan 5, 2013 at 2:16 PM, Ulysses1994XF04 <ben...@comcast.net> wrote:

> I've been wanting to build a small, home biochemistry lab for a while but
> the biggest thing holding me back from pouring the time and money into it is
> that I just can't find any place that will sell the reagents I want to use
> to individuals (most companies that make laboratory-grade reagents will only
> sell to certified labs at universities and companies and such).
>
> I really want to do extractions of cellular products (enzymes, pigments,
> etc) of plants (grass clippings, dead flowers from my garden, etc) and fungi
> and bacteria I can just grow inside. For some of these, I need a good
> hydrophobic extraction solvent that's 1) as pure as possible and 2) has a
> low boiling point so I can easily evaporate/boil off (ideally, I would like
> to recapture it by distillation so I don't have to constantly buy more of
> it).
>
> I can't find a single manufacturer that will sell chloroform,
> dichloromethane or small hydrocarbons like pentane and cyclopentane to
> individuals. What other hydrophobic solvents can I use?
>
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