On Wednesday, May 11, 2011 3:05:20 PM UTC-4, Nathan McCorkle wrote:
Does anyone know of ways other than electrophoresis of DNA to
separate? The problems with long run times and exhaustion of buffer
seem like they could be alleviated... but how? Maybe something like
flow cytometry, but in a much smaller capillary? Maybe the DNA could
be ligated to something like sialyl-lewis-X, and the capillary coated
in CD62P (protein that binds SLX) to slow down the DNA during its
flow... then detection could proceed with UV spectroscopy (the
capillary would need to be very thin to allow differentiation of
single/few molecules)What other ideas do people have?
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
Please have a look at www.ehsystems.com may help your idea. Some instruments are not put on the website as for now because either they are being tested before launch or in prototyping stage, we would still be happy to provide information through email.
nancy@ehsystems.com
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