[DIYbio] Craig Venter synthesis, possible with pcr?

I was checkin out this map of how Craig Venter created a synthetic bacteria cell


http://www.jcvi.org/cms/fileadmin/site/research/projects/first-self-replicating-bact-cell/handouts/image5-lettersize.pdf

Could've this been done with just a hell lot of PCR reactions, PCRing clusters of genes and leaving out the ones that they thought were not needed? Considering all the costs, would it have been cheaper? Also, at the cheapest how much would a PCR reaction be (with and without polymerase)? Any thoughts?

Koeng

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/Uc-e8ygL9jkJ.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment