Hi,
I may get the chance to get to work with a transposon,
however I have a design question:
Do I need to include a terminator? Because the DNA is inserted anywhere in the E.Coli genome, and somewhere afterwards there is a terminator for sure.
For the basical design I'd ligate three PCR products together, and the terminator would be the fourth fragment to attach -> can I save the working time? :D
Would you just go on without?
Best,
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