RE: [DIYbio] Re: DIY DNA synthesis?

Hi Cathy;

Very nice response...Even as a DIY'r, when it is cheaper to simply purchase
than do yourself, then purchase. Let your energy be spent doing things that
have not been done before, or if they have that you can do cheaper, faster
or better than existing technologies.

DNA synthesis is sold for almost the cost of the raw materials. You can
never build a system yourself that will compete in a cost effective manner
with IDT, Operon, Sigma and others. These guys have had years of experience
optimizing their processes and they buy raw materials in such bulk
quantities that their costs per unit are almost zero!

So, it's best to spend your time fixing problems that you can...(but, who
knows, maybe there is a way that I've or nobody else has every thought
about, just with this technology, since it's been around 30 or more years
with a lot of very bright people working in the field for a long time) it's
highly improbable!

My 2 cents in support of Cathy's response.






J Adams

-----Original Message-----
From: diybio@googlegroups.com [mailto:diybio@googlegroups.com] On Behalf Of
Cathal Garvey
Sent: Tuesday, April 02, 2013 5:21 AM
To: diybio@googlegroups.com
Subject: Re: [DIYbio] Re: DIY DNA synthesis?

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This issue crops up again and again; is it DIY if you use convenient
higher-level-abstraction-X?

My opinion is that we should aspire to being *capable* of doing everything
from the ground up, firing our own bricks from sludge, as it were, but that
we shouldn't feel *compelled* to when we don't need to.

So, while I avail happily of DNA synthesis services, because doing it myself
would be very difficult/expensive using presently available methods and
equipment, I aspire to being *able* to do DIY DNA synthesis one way or
another, someday. Even so, when I have a DIY DNA synthesiser on my desk,
I'll still probably order from a company unless my synthesiser is improbably
efficient and easy to use, for the same reason I buy bricks from
brick-sellers*.

*Actually I'd avoid buying bricks because their manufacture is so polluting;
were I to build a house today I'd probably use straw-bale walls for their
low cost, high insulation value, ease of construction and low environmental
impact! DIY FTW.

On 04/01/2013 10:12 AM, Mega wrote:
>> A recent thread on the OpenPCR forum
> <http://openpcr.org/forums/index.php?p=/discussion/21/obtaining-chemis
> try-in-the-uk> was from a guy in the UK who found that the local
> companies who made PCR supplies, ranging from primers to /agarose/,
> would not
> sell him anything. As long as we're dependent on > biotech
> corporations for materials can we truly be "Do-It-Yourself"?
>
>
> When you build a house DIY and you don't burn the bricks from sludge
> - is it still DIY ?
>
>
> :D
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