Agree with Cathal - the most interesting biology will be at the anaerobic anode side of the MFC.
I was working with MFCs a couple years back, and have an idea for isolating electrogenic bacteria that I've never had a opportunity to try out yet. Essentially, you'd build an MFC layer cake inside a Petri dish with a transparent electrode on the bottom (e.g. ITO coating inside a glass Petri dish). Streak bacteria under anaerobic atmosphere onto a slab of agar, and put that slab *bacteria down* on the bottom electrode. Cover the slab with a proton exchange membrane and air cathode. Seal the sides of your layer cake with vaseline to avoid air leaking down to the bacteria.
The idea is that you would be selecting for colonies of bacteria at the bottom of the Petri dish that can oxidize the carbon source in the agar, using the bottom electrode as electron acceptor. You can either short circuit the anode and cathode, or even slightly forward-bias them to pull electrons out of the bacteria (but not so much that you get electrolysis, obviously).
Patrik
PS: have a look at Genspace's anaerobic glove box instructable! http://www.instructables.com/id/DIY-Anaerobic-Chamber-aka-glove-box/
On Sunday, June 23, 2013 2:16:38 PM UTC-7, Cathal Garvey (Android) wrote:
--Be sure to use candle jars for some plates, many of the interesting bugs will be anaerobes. In fact, a DIY glovebox made fairly anoxic would be a good idea for actually dismantling it in the first place, some anaerobes are highly sensitive, die in minutes on exposure.Nathan McCorkle <nmz...@gmail.com> wrote:Portland-folk are taking-apart the mud batteries we made a few months
ago, and we're thinking we'll take a culture. First idea for media is
PDA (potato dextrose agar).
I'm searching for papers now...
--
-Nathan
--
Sent from my Android phone with K-9 Mail. Please excuse my brevity.
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