Re: [DIYbio] Re: Stem Cells out of wisdom teeth

Well "hyper-speed" I think maybe you should slow down because the OP never mentioned modifying cells in anyway. Just culturing them. If my reaction is an over-reaction then other people's reaction is an over and way beyond reaction.

The chances of acquiring some virus such a Hepatitis(which is what Cathal said he was vaccinated from) is probably millions of times(obviously a fictious number) more likely outside of cell culture then inside cell culture. I mean first the cells would need to be infected with the virus in an isolated and semi-sterile? environment. If the virus is randomly infecting the cells that you must eat or inject (most Heps are oral or blood transmission correct?) lets be honest here... If someone should be told not to eat or inject their cell culture I think they are beyond the help of a DIYBio Google group.

Many many people on this group are "Professional" and "Well Educated". Many more so than Cathal or you or I. I try and not prejudge people based on what they think of themselves or what others think of them. Instead I let their actions speak for themselves. I have had significant experience in cell culture and I am sure some on this list have had significantly more than me. All I am saying is that people's reactions seem unreasonable for such a trivial thing. Sure, someone injecting themselves with HeLa cells might really end up in a bad position but the same would happen if someone injected themselves or "pricked" themselves with certain strains of bacteria.

Science to me is about _not_ excluding what one does not trust or does not understand. Convincing people that bacteria and mammalian cell culture is safe starts with the people who are knowledgeable, many of us, so it can propagated to those not knowledgeable, many of the general population. If people on this list are not in support of mammalian cell culture I think that is a big problem because it is an easy place DIYBio can eventually progress to. Whether people want to debate about it or not is fine but I don't think it should be deemed unsafe so fast.


On Monday, June 24, 2013 1:52:52 PM UTC-5, Dan wrote:

On Mon, Jun 24, 2013 at 2:37 PM, Josiah Zayner <josiah...@gmail.com> wrote:
> The people discouraging you to work with cells because you might catch a
> disease are just paranoid and/or have never worked with human cell lines
> before.

Woah, slow down there high-speed.  I'm advocating caution and safety
in the face of a real (if remote) possibility.  The chance that our OP
will actually give himself cancer is small, but the consequences if it
does happen are unacceptably large and the probable reward from his
work is small to non-existent.  Under any risk analysis plan that puts
his plans solidly in the "no-go" category.

> 3. You swallow your own cells and other people's cells all the time.

Your own un-modified cells, sure.  Cells you have removed from your
body and messed with are another issue entirely.

> Cathal that is ridiculous. I have worked with cell culture a ton(HEK, HeLa,
> breast cancer cell lines) and have never had to be vaccinated against
> anything for that purpose. No one I know has been. EU laws maybe at fault?

Cathal is both a professional and a well-educated geneticist; I
wouldn't discard his perspective on this issue so lightly.

-Dan

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