I've been volunteering on a farm that aims for low-input (chems or
work) permaculture. The western Oregon climate is similar to that of
Japan and New Zealand, so lot's of rain and green plants all year
round! The butter is still yellow come winter!
Next week I'm watching/helping with a pig slaughter, so I was thinking
I might try spinning some blood and collecting the plasma and trying
to explant a chunk of heart. I don't have trypsin, though I do have
bromelain, and amazon.com has some digestive tablets that contain
trypsin. Though I don't have micron filters to filter the tablet
solution.
Anyway, I've done this with chicken embryo heart, can't remember what
media I used, but it was probably Eagle or DMEM.
Any ideas for making this work, am I missing something? Will plasma
sustain the cells for a day or so? I think my chicken heart cells
didn't start beating until after a day or two in culture previously.
--
-Nathan
--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CA%2B82U9KqWzVhhBCZzPz7JiLP7a_YgjNxuMC3Mu5HZaKQ6Gxv7g%40mail.gmail.com.
For more options, visit https://groups.google.com/groups/opt_out.
[DIYbio] Animal slaughter -- keeping it alive in a petri
7:35 AM |
Subscribe to:
Post Comments (Atom)






0 comments:
Post a Comment