I didn't know about this half life thing either. But I did add a few AA-s to ends of various proteins for cloning purposes, and all of them worked so far. So I would say adding just a few extra amino acids to the N term should still work most of the time, as long as it starts with Met. :)
On Monday, 7 October 2013 16:47:03 UTC+2, Mega [Andreas Stuermer] wrote:
-- On Monday, 7 October 2013 16:47:03 UTC+2, Mega [Andreas Stuermer] wrote:
Hi everyone!
For expression of a protein I would need to make it fit to the RBS of the vector.
Is it ok, if I attach ATG-GGC (Methionine and Glycine) to the N-Terminus?
So the fusion protein would be Met-Gly-Met-rest of Protein
Is there a guarantee that the protein still works then?
Best,
Andreas
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