[DIYbio] Re: Designing a DIY Gene Electroporator

Adding TQ to this discussion.

Cheers,
--mlp


On Sun, Nov 3, 2013 at 6:43 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
So with a few capacitors like this (microwave capacitor, 2kV 1uF) in
parallel (capacitance adds in parallel):
http://www.ebay.com/itm/BiCai-1-05uF-HV-Microwave-Capacitor-CH85-21105-2100V-AC-New-/171157909970?pt=LH_DefaultDomain_0&hash=item27d9ce71d2

or $1.75 each in packs of 20:
http://www.aliexpress.com/item/Free-Shipping-Imported-microwave-high-voltage-capacitor/1077977860.html

would this $7 7kV power supply be useful, could you drop the voltage
with a divider to get 1.8kV?
http://www.aliexpress.com/item/New-DC-3V-to-7KV-7000V-High-voltage-Generator-Boost-Step-up-Power-Module-free-shipping/1326494805.html

Then seems like all you'd need is an arduino, a gate driver IC, and a
MOSFET or 3.

Are these big clunky caps really the 2013 best-fit part?

On Sun, Nov 3, 2013 at 3:30 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
> Ahh, I found some cheap caps ($10) that are close to brand new and not
> going away any time soon as far as I can tell. Keywords: microwave
> capacitor.
>
> On Ebay I was seeing ~2kv ~1uF caps for $10, they're much larger than
> that MOSFET electroporator's 'energy storage capacitor' though...
> which he only lists as 3.5kV 5uF, which doesn't give me any decent
> results on google.
>
> http://www.ebay.com/itm/BiCai-1-05uF-HV-Microwave-Capacitor-CH85-21105-2100V-AC-New-/171157909970?pt=LH_DefaultDomain_0&hash=item27d9ce71d2
>
> On Fri, Oct 18, 2013 at 2:01 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
>> What about the issue of finding uF sized caps for 1.8-2.5kV? Is there
>> a way to simulate a cap's discharge? Use inductors or something?
>>
>> On Tue, Nov 8, 2011 at 2:03 PM, John Griessen <john@industromatic.com> wrote:
>>> On 11/08/2011 03:16 PM, Nathan McCorkle wrote:
>>>>
>>>> When I took apart a BioRad Gene Pulser II, it was loaded with
>>>> SCRs...
>>>
>>>
>>> Sure.  They're a natural for the below 500Volt parts of the switched
>>> capacitor pulser.
>>> And for the 2500Volt part, a transformer and some HV diodes, and maybe a HV
>>> relay
>>> to protect a pre conditioner (optional equipment)
>>>
>>>
>>> John
>>>
>>> --
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>>
>>
>>
>> --
>> -Nathan
>
>
>
> --
> -Nathan



--
-Nathan

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