I thought isothermal PCR doesn't require a thermal cycler.
LAMP is an isothermal nucleic acid amplification. In contrast to the polymerase chain reaction (PCR) technology in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature, and does not require athermal cycler.
Smart Dart looks a bit like bullshit. They never even say what the
device actually does, they also do not explain how isothermal dna
amplification works, and that it's different (and more expensive) from
usual PCR. Since it does not require cycling, does it mean that the
device is just a vastly overpriced thermal block with Android control?
In this case, it sounds more like borderline fraud.
Open Trons looks really cool though, considering it's got reasonable
precision and they actually make the hardware open - I'd love to use
it for tedious stuff like serial dilutions.
On Wed, Nov 12, 2014 at 2:34 AM, Kevin Chen <bkevinchen@gmail.com> wrote:4/5 funded! Go Opentrons!Opentrons: ~50k/100khttps://www.kickstarter.com/projects/932664050/opentrons-open-source-rapid-prototyping-for-biologminiPCR: Funded,~60k/20khttps://www.kickstarter.com/projects/minipcr/minipcr-a-dna-discovery-system-for-everyoneOpenQPCR: Funded ~122k/50khttps://www.kickstarter.com/projects/chaibio/open-qpcr-dna-diagnostics-for-everyoneBiodidact DNA barcoding project: Funded 1577/1500https://www.mainstreetcrowd.com/projects/DNA-barcoding-project/Smart-Dart: Funded ~22k/20khttps://www.indiegogo.com/projects/smart-dart-platform-dna-amplification-for-allOn Sat, Nov 8, 2014 at 2:27 PM, Will Canine <willcanine@gmail.com> wrote:Thanks Dakota! :)Emphatically YES to your the tube/plate manipulation and cooling/heating,in multiple ways.First, we are working hard to make it easy to incorporate other openhardware automation modules into OpenTrons jobs. Basically, if you make atool (anything, microscope, plate hotel, whatever you want) and incorporateUSB or BLE communication + create a tool profile on Mix.Bio, people caneasily add it to their automated workflow. We are really excited to workwith all our Kickstarter backers/early adopters to build this out in a waythat works for their development on the platform.Second, we already have prototypes of a tube gripper, plate arm (we areworking with the uArm team in Shenzhen), temperature controlled racks, and acentrifuge. We will be releasing these first four modules in Summer 2015.Excited to work with you all! :)WillOn Friday, November 7, 2014 1:22:05 PM UTC-8, Dakota wrote:But in all honesty I'm very happy for the people who are getting fundedto make these cool new machines, it's the start of something awesome.OpenTrons looks pretty sweet to me now and has grown on me after I keepre-watching the video.In the future do you think it'll be able to pick up tubes / plates andmove them around? Or have cooled/heated racks?On Fri, Nov 7, 2014 at 3:58 PM, Koeng <koen...@gmail.com> wrote:350 for a single well pcr machine...On Friday, November 7, 2014 12:32:11 PM UTC-8, Dakota wrote:good reads, I like the single well DNA Dart because #$%@ controlsOn Fri, Nov 7, 2014 at 3:26 PM, Will Sutton <wsut...@gmail.com> wrote:and this one:https://www.indiegogo.com/projects/smart-dart-platform-dna-amplification-for-allOn Wednesday, November 5, 2014 1:20:38 AM UTC-5, Kevin Chen wrote:'Tis the season?https://www.kickstarter.com/projects/932664050/opentrons-open-source-rapid-prototyping-for-biologhttps://www.kickstarter.com/projects/minipcr/minipcr-a-dna-discovery-system-for-everyonehttps://www.kickstarter.com/projects/chaibio/open-qpcr-dna-diagnostics-for-everyoneAny others?---- You received this message because you are subscribed to the GoogleGroups DIYbio group. To post to this group, send email todiy...@googlegroups.com. 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For more options, visit this group athttps://groups.google.com/d/forum/diybio?hl=enLearn more at www.diybio.org---You received this message because you are subscribed to a topic in theGoogle Groups "DIYbio" group.To unsubscribe from this topic, visithttps://groups.google.com/d/topic/diybio/iExbhTf959E/unsubscribe.To unsubscribe from this group and all its topics, send an email todiybio+unsubscribe@googlegroups.com.To post to this group, send email to diybio@googlegroups.com.Visit this group at http://groups.google.com/group/diybio.To view this discussion on the web visithttps://groups.google.com/d/msgid/diybio/034a9a9a-29f2-4e39-859c-20ee3444c43e%40googlegroups.com.For more options, visit https://groups.google.com/d/optout.--Kevin ChenHyasynth Bio: COO, Co-founderBricobio: Co-founder@bkevinchen---- You received this message because you are subscribed to the Google GroupsDIYbio group. 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