I can second IDT gBlocks for being best for small genes
-- In my experience, genescript is very very good at complex genes. If you want to synthesize like a terminator or a CRISPR repeat, they can do it. I once got a 30bp inverted repeat separated by about 20bp, which was notoriously hard to sequence, but they still got it out of production faster than I could actually succeed in sequencing it after I got it. Their service is quick, and I've used them a couple times without any hassle. Recommended!
I believe gen9 has cheaper synthesis rate... but I've had a lot of problems with them in the past
IDT has the best rates for over 60bp IF you buy in bulk... For smaller orders invitrogen does much cheaper ~100bp primers. But beware! They are cheaper but not as pure. (In my experience, havent tried many other companies)
For text messages, I think gBlocks are best for price
-Koeng
On Friday, January 9, 2015 at 4:46:16 PM UTC-8, Patrik D'haeseleer wrote:
On Friday, January 9, 2015 at 4:46:16 PM UTC-8, Patrik D'haeseleer wrote:
I'll post some of my own favorites to start:
- uBiome (of sequence-your-poop fame) offers microbiome sequencing for $89/sample, no preprocessing required. Of course, they're aimed primarily at human microbiome, but you can send them pretty much any mixed bacterial sample, and they'll do 16S sequencing and send you the results. Not sure if they can do ITS for mixed fungal samples as well; worth talking to them about that.
- IDT's gBlocks is currently one of the best deals to get smallish genes synthesized: $89 for 500bp, up to $329 for 2000bp!
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