Re: [DIYbio] Re: Best/Cheapest Services for DNA Sequencing & Synthesis

On Sat, Jan 10, 2015 at 1:28 AM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
> Any recommendations for bacterial genomes?
>
> You should be able to multiplex 96 genomes on a single MiSeq lane, so the
> cost will be mostly determined by library generation. rather than the
> sequencing itself.
>
> It'd be great if there was some service where you can order just a single
> bacterial genome sequenced, library generation and all. Most NGS sequencing
> facilities aren't going to want to put up with the overhead of selling
> 1/96th of a lane though...
>

Can you explain what you mean by a single MiSeq lane? Our lab has Life
Tech Ion Protons so I'm a little familiar with that. It's not my area
so I only know a little bit about the NGS setup. I know my colleagues
do exome seq on the chips, and I think they can run 16 (or was it 8 or
4, can't remember) different samples on a chip using barcode adapters,
but I think that limits how many reads they can do per sample. As long
as the customer is willing to work with us, I think we try to tackle
most projects, being a startup company and all.

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