Re: [DIYbio] Dynamic Sculpture Fungi

On Mon, Feb 23, 2015 at 9:33 AM, Dakota Hamill <dkotes@gmail.com> wrote:
> Yeah let me replate them on PDA this week and make sure they're contaminant
> free, message me in 3 days if I don't get back to you

These are /quite/ easy to isolate at home, using a few cents worth of
mushroom tissue from your local grocery store's bulk section.

Grab a scalpel/straight-razor, some alcohol, few jars of sterile
water, and a flame source. dunk the mushroom in alcohol/bleach... then
rinse several times with sterile water (pour water over mushroom chunk
so it falls into a waster jar), then like you're a lumberjack milling
a nice board from a tree... cut faces on the tissue (CLEANING the
blade with alcohol and a flame ON EACH CUT). Remember that any surface
contaminants will drag with the blade during each cut, after you've
squared up the faces, take another layer or two off (again with a
clean blade each time) then just plop a layer/chunk down onto PDA and
you should be good-to-go.

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