Hi Everyone,
-- Here are some updates on some of the preliminary research I have done.
1. Identified preliminary targets for better crops (biofortification, resistance to fungus/viruses, resistance to sucking pests, drought tolerance, temperature tolerance) http://www.sciencemag.org/content/327/5967/812.full#T1 and https://royalsociety.org/~/media/Royal_Society_Content/policy/publications/2009/4294967719.pdf are some interesting reviews of what sort of technological properties are needed and possible to feed our growing population.
2. Chose biofortification over temperature tolerance. My guess is it is going to be easier to create agricultural cultivars that express a vitamin than it is to express an antifreeze protein(s).
3. Chose what to be expressed. I am going to start with a GFP and herbicide tolerance and go from there; I will decide on the vitamin later. If anyone has any recommendations please let me know.
4. Chose model organism (maize aka corn) Although rice and wheat are more widely consumed; the maize genome has been fully sequenced and there is more support for it.
5. Found protocols for raising maize http://cshprotocols.cshlp.org/content/2009/10/pdb.emo132.full
My next steps are:
1. Get some maize cultivars
2. Find a place to grow them
3. Grow maize cultivars
4. Chose protocol for transformation
On Sunday, August 9, 2015 at 2:43:51 AM UTC+9, John D wrote:
On Sunday, August 9, 2015 at 2:43:51 AM UTC+9, John D wrote:
Hello Fellow Biohackers,I want to work on creating cold resistant forms of wheat. I don't have a lot of experience working with plants; mostly I have done ecology research. I have done some work in phylogenetics as well. If anyone has any resources, learning materials, etc, or know of any labs in the Boston area that I could work with that would be wonderful!Best,John
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