*mega unfortunately the tRNA method uses pol III promoters as is applicable for my application. My interrest in the ribozyme construct is expression of unmodified gRNA transcript utilizing Pol II promoters.
Den fredag den 20. november 2015 kl. 23.49.39 UTC-8 skrev Mega [Andreas Stuermer]:
-- Basically all these methods are from around the year 2000 when there was i huge interest in producing RNA transcript for investigating HIV and later similar methods were used in RNAi. Som interesting reads are -
General plasmids for producing RNA in vitro transcripts with homogeneous ends, Expression of siRNA from a single transcript that includes multiple ribozymes in mammalian cells.General Acid-Base Catalysis in the Mechanism of a Hepatitis Delta Virus Ribozyme, etc.
Den fredag den 20. november 2015 kl. 23.49.39 UTC-8 skrev Mega [Andreas Stuermer]:
Hi, personally haven't used it yet. Too much other stuff to do at the moment. Btw there's a new method published which uses a tRNA which is shorter than two ribozymes and spacer
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