Re: [DIYbio] DNA synthesis at 10 cents per letter

gBlocks from IDT are ~$0.15 /bp. They go up to 2kb, and I've had good luck with them.

On Sun, Nov 22, 2015 at 1:31 PM Francis Lee <franci...@gmail.com> wrote:

maybe for oligos, but certainly not for gene synthesis.

IDT ultramers which maxes out @ 200 bp go for ~ 0.16/bp if you order in bulk.

Genscript is @ 0.35/bp for gene synthesis

On Sat, Nov 21, 2015 at 8:50 PM, Matt Lawes <ma...@insysx.com> wrote:

What am I missing? I thought prices have been 10c a base for a while .....

Sent from my T-Mobile Android device

On Nov 21, 2015 8:30 PM, Brian Degger <brian....@gmail.com> wrote:

Why diy at 10cpb?cheap enough for 30c per aa. A 100aa peptide is $300 l (plus promoters etc)

On 21 Nov 2015 23:27, "technologiclee" <technol...@gmail.com> wrote:

http://www.wired.com/2015/11/making-dna/
"When Twist launches its beta program in 2016, it will offer gene synthesis at 10 cents per letter with a guaranteed turnaround time of 10 days."

"Moreover, the silicon wafer is cleverly optimized for the second step of gene synthesis—the stitching of oligos together—because Twist's engineers figured out how to cut down on moving tiny volumes of liquid. Twist's proprietary machine, a small-car sized system that WIRED wasn't allowed to photograph, deposits one oligo into each of the 100 or so holes inside of a nanowell."

Is there any DIY system capable of this? What would it take to do that? What is the basic mechanism of moving the fluid in this system?

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