The modularity issues I mentioned aren't to do with the payload (nuclease vs. Integrase vs. Tr-factor), they relate to the actual binding efficacy of multi-finger ZFs.
ZFs are unfortunately context sensitive in uncomfortable ways. Sangamo *claimed* to have algorithms to resolve this, but everyone else ended up using massive a degeneracy strategies like phage display to artificially evolve functional multi-finger modules. It worked, but was labour dependent and required a kit that required onerous MTAs to acquire.
Source: a PhD I started years ago when ZFs were still cool that ultimately abandoned ZFs before falling off a cliff.
On 5 November 2015 11:57:58 GMT-08:00, Yuriy <yuriyology@gmail.com> wrote:
Who mentioned ZFNs? I only mentioned ZFRs. Why get twisted over patents? There is no mention of needing to commercialize this intergration. Is there?
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Sent from my Android device with K-9 Mail. Please excuse my brevity.
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