Re: [DIYbio] Synechococcus elongatus UTEX 2973

Very cool!

Looks like there is a site that would sell it, but they're out of stock:
http://utex.org/products/algae-express-utex-2973-synechococcus-elongatus

interestingly that page says it was mutated from this strain which was
isolated in 1952:
http://utex.org/products/utex-0625

That page has a seemingly informative blurb, and mentions this is produced:
https://en.wikipedia.org/wiki/Metallothionein
which seems like a sort of chelator... so I'd guess at least that
these proteins would need cooked or denatured somehow, so they didn't
interfere with digestion if this was used as food.

On Thu, Dec 10, 2015 at 10:50 AM, Jonathan Cline <jcline@ieee.org> wrote:
> These cyanobacteria in general are freshwater photoautotrophs.
> Might this new little guy be edible after washing?
>
>
>
> Quote:
>
> Efforts focusing on understanding cyanobacterial
> systems are underway; however, one drawback compared to E. coli or yeast is
> that the growth rates of commonly
> used cyanobacterial model strains are significantly slower, requiring
> extended timeframes (weeks to months) to
> accomplish synthetic biology experiments that can be performed in E. coli or
> yeast in days.
> [...]
> Growth of Synechococcus UTEX 2973 was assessed under different
> conditions and the shortest doubling time was 1.9 hrs in BG11
> medium19 at 41uC under continuous 500 mmoles photons?m22?s21
> white light with 3%CO2. This is a remarkably high growth rate under
> autotrophic condition, the highest rate reported to date for a
> cyanobacterial strain, and comparable to heterotrophic growth
> rates of the yeast S. cerevisiae. The doubling time of Synechococcus
> UTEX 2973 increased to 2.3 hrs at 38uC (Table 1).
> [...]
> Culture conditions that are optimal for the rapid growth of
> Synechococcus UTEX 2973 (38–41uC, 3% CO2 and 500 mmole
> photons?m22?s21 using BG11 media) are utilized in many laboratories,
> and no special nutrients, such as vitamins, are required for the
> growth of this cyanobacterial strain. This strain grows so rapidly, in
> culture volumes ranging from 50 ml to 100 L, such as in a photobioreactor,
> that contamination was not apparent even when growth
> media were not sterilized and the systems were semi-open to the
> outside. On solid BG11 plates, at 38uC and under 200 mmoles
> photons?m22?s21 light and in ambient air, single colonies of
> Synechococcus UTEX 2973 were visible within 2 days after plating
> from a very dilute liquid culture, and these colonies were large
> enough for passage by the third day.
> [...]
>
>
> End Quote
>
> Ref:
>
> Yu, Jingjie; Liberton, Michelle; Cliften, Paul F.; Head, Richard D.; Jacobs,
> Jon M.; Smith, Richard D.; Koppenaal, David W.; Brand,
> Jerry J.; and Pakrasi, Himadri B., ,"Synechococcus elongatus UTEX 2973, a
> fast growing cyanobacterial chassis for biosynthesis using
> light and CO2." Scientific Reports.5,. 8132. (2015).
> http://digitalcommons.wustl.edu/open_access_pubs/4285
>
> --
>
> ## Jonathan Cline
> ## jcline@ieee.org
> ## Mobile: +1-805-617-0223
> ########################
>
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--
-Nathan

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